Results 51 to 60 of about 103,280 (234)
Macromolecular Crowding Modulates Folding Mechanism of α/β Protein Apoflavodoxin [PDF]
, 2009 Protein dynamics in cells may be different from those in dilute solutions in vitro, because the environment in cells is highly concentrated with other macromolecules.
Cheung, Margaret S. +7 more
core +1 more source
, 2013 Macromolecular crowding is an ubiquitous phenomenon in living cells that significantly affects the function of enzymes invivo. However, this effect has not been paid much attention in the research of the immobilization of enzymes onto mesoporous silica ...
Jin, Qianru +7 more
core +1 more source
Macromolecular crowding: Sensing without a sensor
Current Opinion in Cell Biology, 2023 All living cells are crowded with macromolecules. Crowding can directly modulate biochemical reactions to various degrees depending on the sizes, shapes, and binding affinities of the reactants. Here, we explore the possibility that cells can sense and adapt to changes in crowding through the widespread modulation of biochemical reactions without the ...
Holt, Liam, Delarue, Morgan
openaire +4 more sources
Nature Communications, 2021 Cells experience various osmotic perturbation, but cellular osmosensing mechanisms remain obscure. Here, the authors report that cells recognize osmotic stress from the inside through macromolecular crowding-driven and poly(ADP-ribose)-conditioned liquid–
Kengo Watanabe +7 more
doaj +1 more source
Macromolecular crowding induces holo α-lactalbumin aggregation by converting to its apo form.
PLoS ONE, 2014 Macromolecular crowding has been shown to have an exacerbating effect on the aggregation propensity of amyloidogenic proteins; while having an inhibitory effect on the non-amyloidogenic proteins.
Shruti Mittal +1 more
doaj +1 more source
Protein folding by the effects of macromolecular crowding [PDF]
Protein Science, 2004 Abstract Unfolded states of ribonuclease A were used to investigate the effects of macromolecular crowding on macromolecular compactness and protein folding. The extent of protein folding and compactness were measured by circular dichroism spectroscopy, fluorescence correlation spectroscopy, and NMR spectroscopy in the presence of ...
Nobuhiko, Tokuriki +6 more
openaire +2 more sources
FEBS Open Bio, EarlyView.Hyperosmotic stress triggers the relocation of the CFIm complex from the nucleus to the cytoplasm. This shift creates a nuclear ‘stoichiometric bottleneck’, limiting CFIm availability for mRNA processing. Consequently, specific mRNAs like NUDT21 and DICER1 undergo targeted 3′UTR shortening, demonstrating how spatial protein dynamics drive rapid ...
Hitomi Soumiya +2 more
wiley +1 more source
Regulation of Actin Bundle Mechanics and Structure by Intracellular Environmental Factors
Frontiers in Physics, 2021 The mechanical and structural properties of actin cytoskeleton drive various cellular processes, including structural support of the plasma membrane and cellular motility.
Nicholas Castaneda +6 more
doaj +1 more source
CaMKII binding to GluN2B is differentially affected by macromolecular crowding reagents.
PLoS ONE, 2014 Binding of the Ca2+/calmodulin(CaM)-dependent protein kinase II (CaMKII) to the NMDA-type glutamate receptor (NMDAR) subunit GluN2B controls long-term potentiation (LTP), a form of synaptic plasticity thought to underlie learning and memory.
Dayton J Goodell +3 more
doaj +1 more source
Advanced Functional Materials, EarlyView.Versatile guanosine‐quadruplex (GQ) hydrogels are developed as dynamic biomatrices for cell culture. These scaffolds are fabricated under mild aqueous conditions, present improved stability, tunable mechanical properties within physiological ranges; fibrillar, porous microstructure, ion conductivity, and cytocompatibility.
Neha Thakur +10 more
wiley +1 more source