Insights on the effect of macromolecular crowding on transcription and its regulation. [PDF]
QRB DiscovXu W +4 more
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Macromolecular crowding limits growth under pressure
Nature Physics, 2021 Cells that grow in confined spaces eventually build up mechanical compressive stress. This growth-induced pressure decreases cell growth. Growth-induced pressure is important in a multitude of contexts, including cancer1–3, microbial infections4 and ...
Baptiste Alric +4 more
semanticscholar +1 more source
Macromolecular Crowding Modulates Actomyosin Kinetics [PDF]
Biophysical Journal, 2016 Actomyosin kinetics is usually studied in dilute solutions, which do not reflect conditions in the cytoplasm. In cells, myosin and actin work in a dense macromolecular environment. High concentrations of macromolecules dramatically reduce the amount of free space available for all solutes, which results in an effective increase of the solutes' chemical
Jinghua, Ge +4 more
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Conformation-changing enzymes and macromolecular crowding
Physical Chemistry Chemical Physics, 2021 We study how crowding affects the activity and catalysis-enhanced diffusion of enzymes and passive tracers by employing a fluctuating-dumbbell model of conformation-changing enzymes.
Tomasz Skóra +2 more
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A combined physicochemical approach towards human tenocyte phenotype maintenance
Materials Today Bio, 2021 During in vitro culture, bereft of their optimal tissue context, tenocytes lose their phenotype and function. Considering that tenocytes in their native tissue milieu are exposed simultaneously to manifold signals, combination approaches (e.g.
C.N.M. Ryan +9 more
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All atom insights into the impact of crowded environments on protein stability by NMR spectroscopy
Nature Communications, 2020 The precise effects of crowding on protein folding have been difficult to establish. Here the authors apply multidimensional high-resolution NMR spectroscopy to provide insight on the local impact of macromolecular crowding on the thermodynamic stability
Birgit Köhn, Michael Kovermann
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Macromolecular Crowding In Vitro , In Vivo , and In Between [PDF]
Trends in Biochemical Sciences, 2016 Biochemical processes take place in heterogeneous and highly volume-occupied or crowded environments that can considerably influence the reactivity and distribution of participating macromolecules. We summarize here the thermodynamic consequences of excluded-volume and long-range nonspecific intermolecular interactions for macromolecular reactions in ...
Germán, Rivas, Allen P, Minton
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Misfolding of transthyretin in vivo is controlled by the redox environment and macromolecular crowding. [PDF]
J Biol ChemJayaweera SW +9 more
europepmc +2 more sources
Macromolecular Crowding: a Hidden Link Between Cell Volume and Everything Else.
Cellular Physiology and Biochemistry, 2021 High density of intracellular macromolecules creates a special condition known as macromolecular crowding (MC). One well-established consequence of MC is that only a slight change in the concentration of macromolecules (e.g., proteins) results in a shift
M. Model +2 more
semanticscholar +1 more source
The effect of macromolecular crowding on the electrostatic component of barnase-barstar binding: a computational, implicit solvent-based study. [PDF]
PLoS ONE, 2014 Macromolecular crowding within the cell can impact both protein folding and binding. Earlier models of cellular crowding focused on the excluded volume, entropic effect of crowding agents, which generally favors compact protein states.
Helena W Qi +3 more
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