Results 71 to 80 of about 6,829 (247)

Crowding Induces Entropically-Driven Changes to DNA Dynamics That Depend on Crowder Structure and Ionic Conditions

Frontiers in Physics, 2018
Macromolecular crowding plays a principal role in a wide range of biological processes including gene expression, chromosomal compaction, and viral infection.
Warren M. Mardoum, Stephanie M. Gorczyca, Kathryn E. Regan, Tsai-Chin Wu, Rae M. Robertson-Anderson   +4 more
doaj   +1 more source

Oligopeptides/DNA Coacervate Droplets as Macromolecular Delivery Microcarriers

Advanced Science, EarlyView.
Oligopeptide–DNA coacervates formed via liquid–liquid phase separation serve as programmable carriers for macromolecular cargos, entering cells through lipid raft–associated pathways and subsequently undergoing enzyme triggered intracellular disassembly driven by DNA degradation processes, thereby enabling efficient, controlled, and spatially defined ...
Linyi Zhang, Chong Wang, Mengqi Han, Yi Yang, Teng Ma, Luoran Shang   +5 more
wiley   +1 more source

A Sensor for Quantification of Macromolecular Crowding in Living Cells [PDF]

Biophysical Journal, 2015
Macromolecular crowding in cells influences processes such as folding, association and diffusion of proteins and polynucleic acids. Direct spatiotemporal readout of crowding would be a powerful approach for unraveling the structure of the cytoplasm and determining the impact of excluded volume on protein function in living cells.
Boersma, Arnold J., Zuhorn, Inge S., Poolman, Bert   +2 more
openaire   +3 more sources

Multifactorial bioengineering in the tendon context to maintain tenocyte phenotype and to direct dermal fibroblasts towards tenogenic lineage

Bioactive Materials
Considering that cells in vivo are simultaneously exposed to multiple signals, multifactorial bioengineering slowly but surely becomes the state of play in controlling cell function in vitro. In this context, herein we assessed the simultaneous effect of
Adrian Djalali-Cuevas, Mandy Rettel, Frank Stein, Mikhail Savitski, Ioannis Gkiatas, Anastasios Korompilias, Ioannis Skoufos, Athina Tzora, Nikitas Prassinos, Nikolaos Diakakis, Dimitrios I. Zeugolis   +10 more
doaj   +1 more source

TDP‐43 Aggregation: The Healthy‐Toxic Balance of the Prion‐Like Domain

Advanced Science, EarlyView.
TDP‐43 function relies on a delicate balance between reversible phase‐separated states and irreversible aggregation. Under physiological conditions, TDP‐43 forms dynamic droplets and oligomers that support normal cellular functions. In pathological contexts, this balance shifts toward aberrant aggregation, leading to toxic species.
Luca Zangrando, Emanuele Buratti, Francesca Paron   +2 more
wiley   +1 more source

The unfolding of iRFP713 in a crowded milieu [PDF]

PeerJ, 2019
The exploring of biological processes in vitro under conditions of macromolecular crowding is a way to achieve an understanding of how these processes occur in vivo.
Olesya V. Stepanenko, Olga V. Stepanenko, Irina M. Kuznetsova, Konstantin K. Turoverov   +3 more
doaj   +2 more sources

Crowding-facilitated macromolecular transport in attractive micropost arrays

Scientific Reports, 2017
Our study of DNA dynamics in weakly attractive nanofabricated post arrays revealed crowding enhances polymer transport, contrary to hindered transport in repulsive medium.
Fan-Tso Chien, Po-Keng Lin, Wei Chien, Cheng-Hsiang Hung, Ming-Hung Yu, Chia-Fu Chou, Yeng-Long Chen   +6 more
doaj   +1 more source

Brownian Dynamics Computational Model of Protein Diffusion in Crowded Media with Dextran Macromolecules as Obstacles

Entropy, 2017
The high concentration of macromolecules (i.e., macromolecular crowding) in cellular environments leads to large quantitative effects on the dynamic and equilibrium biological properties.
Pablo M. Blanco, Mireia Via, Josep Lluís Garcés, Sergio Madurga, Francesc Mas   +4 more
doaj   +1 more source

Macromolecular Crowding of Lipocalins

The FASEB Journal, 2007
Lipocalins are a family of proteins with a tertiary structure of a beta barrel core with alpha‐helices external to the barrel core and normally two disulfide bonds. The lipocalins are an important small molecule binding protein. The lipocalins can be very diverse in overall amino acid sequence (<40%sequence homology) just maintaining core amino ...
openaire   +1 more source

Antibody binding reports spatial heterogeneities in cell membrane organization

Nature Communications, 2023
The spatial organization of cell membrane glycoproteins and glycolipids is critical for mediating the binding of ligands, receptors, and macromolecules on the plasma membrane.
Daniel P. Arnold, Yaxin Xu, Sho C. Takatori   +2 more
doaj   +1 more source