Results 71 to 80 of about 103,280 (234)

Large-scale analysis of macromolecular crowding effects on protein aggregation using a reconstituted cell-free translation system

Frontiers in Microbiology, 2015
Proteins must fold into their native structures in the crowded cellular environment, to perform their functions. Although such macromolecular crowding has been considered to affect the folding properties of proteins, large-scale experimental data have so
Tatsuya eNiwa, Ryota eSugimoto, Lisa eWatanabe, Shugo eNakamura, Takuya eUeda, Hideki eTaguchi   +5 more
doaj   +1 more source

Harnessing Phase Separation for the Development of High‐Performance Hydrogels

Advanced Science, EarlyView.
ABSTRACT Hydrogels are indispensable for the development of next‐generation bioelectronics, soft robotics, and biomedical devices, where their mechanical properties determine performance and reliability. Among strategies to enhance hydrogel mechanics, phase separation enables controlled heterogeneity resulting in gel networks that are reinforced by ...
Yue Shao, Yiming Ma, Baihao Shao, Molly M. Stevens   +3 more
wiley   +1 more source

The Effect of Attractive Interactions and Macromolecular Crowding on Crystallins Association

, 2016
In living systems proteins are typically found in crowded environments where their effective interactions strongly depend on the surrounding medium. Yet, their association and dissociation needs to be robustly controlled in order to enable biological ...
Song, F (reprint author), Chinese Acad Sci, Inst Mech, State Key Lab Nonlinear Mech LNM, Beijing 100190, Peoples R China., Curk T, Wei JC(韦佳辰), Dobnikar J, Song F(宋凡)   +4 more
core   +1 more source

Macromolecular crowding as a suppressor of human IAPP fibril formation and cytotoxicity. [PDF]

PLoS ONE, 2013
The biological cell is known to exhibit a highly crowded milieu, which significantly influences protein aggregation and association processes. As several cell degenerative diseases are related to the self-association and fibrillation of amyloidogenic ...
Janine Seeliger, Alexander Werkmüller, Roland Winter   +2 more
doaj   +1 more source

Aberrant GRP78 Phase Transition Sustains Endothelial IRE1α Signaling and Drives Blood–Brain Barrier Failure in Cerebral Amyloid Angiopathy

Advanced Science, EarlyView.
Vascular Aβ40 corrupts GRP78 phase behavior in brain endothelial cells, sustaining IRE1α–TRAF2–JNK signaling and driving apoptosis, tight junction loss, and blood–brain barrier failure in cerebral amyloid angiopathy. Pharmacological IRE1α inhibition restores vascular integrity, reduces leakage, and improves functional outcomes, revealing a targetable ...
Honglin Zheng, Qiang Li, Haiyang Luo, Yapei Yuan, Na Zhang, Yongting Lu, Suying Duan, Jieshi Zhong, Hang Zhang, Chenyang Liu, Yaochong Zhang, Wenzhuo Zhao, Yaxuan Song, Tuo Wang, Si Yan, Yapeng Li, Han Liu, Yuan Gao, Zongping Xia, Yuming Xu   +19 more
wiley   +1 more source

Impact of macromolecular crowding on DNA replication [PDF]

, 2014
Enzymatic activities in vivo occur in a crowded environment composed of many macromolecules. This environment influences DNA replication by increasing the concentration of the constituents, desolvation, decreasing the degrees of freedom for diffusion and
Richardson, Charles, Lee, Seung–Joo, Wagner, Gerhard, Akabayov, Sabine R., Akabayov, Barak   +4 more
core   +1 more source

Chromatin Packing Domain Engineering Through the Manipulation of Nuclear Cationic States

Advanced Science, EarlyView.
Manipulation of intranuclear divalent cation concentrations rapidly and reversibly remodels chromatin packing domains in living cells. Magnesium depletion disrupts domain compaction, heterochromatin organization, and transcriptional regulation, whereas magnesium loading promotes more compact and stable domains.
Cody L. Dunton, Carrillo Paola Gonzalez, Luay Almassalha, Wing Shun Li, Ruyi Gong, Nicolas Acosta, I Chae Ye, Jane Frederick, Christopher Hogg, Saira John, J. A. Gareth Williams, Rikkert J. Nap, Igal Szleifer, Vadim Backman   +13 more
wiley   +1 more source

Crowding Induces Entropically-Driven Changes to DNA Dynamics That Depend on Crowder Structure and Ionic Conditions

Frontiers in Physics, 2018
Macromolecular crowding plays a principal role in a wide range of biological processes including gene expression, chromosomal compaction, and viral infection.
Warren M. Mardoum, Stephanie M. Gorczyca, Kathryn E. Regan, Tsai-Chin Wu, Rae M. Robertson-Anderson   +4 more
doaj   +1 more source

How does domain replacement affect fibril formation of the rabbit/human prion proteins. [PDF]

PLoS ONE, 2014
It is known that in vivo human prion protein (PrP) have the tendency to form fibril deposits and are associated with infectious fatal prion diseases, while the rabbit PrP does not readily form fibrils and is unlikely to cause prion diseases.
Xu Yan, Jun-Jie Huang, Zheng Zhou, Jie Chen, Yi Liang   +4 more
doaj   +1 more source

Soft Interactions, Rather Than Crowder Size, Determine Deviations From Arrhenius‐Like Kinetics of Phosphofructokinase‐1

Angewandte Chemie, EarlyView.
Our study investigates macromolecular crowding effects in the Arrhenius‐like and non‐Arrhenius temperature regimes of the human platelet isoform of phosphofructokinase‐1 (PFKP). The results reveal how PFKP maintains temperature robustness of catalytic activity in differently crowded environments.
Harshita Rastogi, Madhav Samanta, Truong‐An Nguyen, Mehrnoosh Khodam Hazrati, Bradley A. Webb, Eva Pluhařová, Stepan Timr, Simon Ebbinghaus   +7 more
wiley   +2 more sources