Results 91 to 100 of about 103,280 (234)
Advanced Science, EarlyView.Ex‐spRandom is a spatial transcriptomics platform that synergizes random‐primed chemistry with iterative hydrogel expansion. By physically decrowding the dense FFPE matrix, this scalable technology shatters the traditional resolution‐sensitivity barrier.
Shunji Zhang +7 more
wiley +1 more source
Macromolecular Crowding as a Cell VolumeSensor
Cellular Physiology and Biochemistry, 2000 The non-ideal properties of solutions containing high concentrations of macromolecules can result in enormous increases in the activity of the individual macromolecules. There is considerable evidence that macromolecular crowding and confinement not only occur in cells, but that these are major determinants of the activity of the proteins and other ...
openaire +2 more sources
Localization of protein aggregation in Escherichia coli is governed by diffusion and nucleoid macromolecular crowding effect. [PDF]
PLoS Computational Biology, 2013 Aggregates of misfolded proteins are a hallmark of many age-related diseases. Recently, they have been linked to aging of Escherichia coli (E. coli) where protein aggregates accumulate at the old pole region of the aging bacterium.
Anne-Sophie Coquel +8 more
doaj +1 more source
Advanced Science, EarlyView.The effect of ligand reactivity and carrier mechanics on thiol‐mediated uptake was investigated at the single‐molecule/particle level. The interaction kinetics of LA and AspA with membrane thiols were quantified; AspA is more efficient due to the higher binding affinity, greater binding stability, and more double‐disulfide binding, as well as greater ...
Zhuang Zhang +7 more
wiley +1 more source
Macromolecular Crowding of Lipocalins
The FASEB Journal, 2007 Lipocalins are a family of proteins with a tertiary structure of a beta barrel core with alpha‐helices external to the barrel core and normally two disulfide bonds. The lipocalins are an important small molecule binding protein. The lipocalins can be very diverse in overall amino acid sequence (<40%sequence homology) just maintaining core amino ...
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Advanced Science, EarlyView.CDegSR, a new tool for imaging RNA at single molecule level in living cells was developed by researchers. Background noise was eliminated by protein engineering and making it degrade when not bound to its target. This allowed individual RNA molecules to be clearly imaged and tracked.
Shipeng Shao, Hongchen Zhang
wiley +1 more source
Advanced Science, EarlyView.Phase separation of SF3B1 acts as a key regulatory mechanism for dynamic alternative splicing throughout early mouse embryogenesis. Its absence triggers extensive splicing errors, which induce persistent DNA damage, defective cell cycle progression, and failed cell lineage commitment, and ultimately hinder the normal growth and development of ...
Kang Zhao +15 more
wiley +1 more source
Journal of Tissue Engineering, 2017 Biomimetic microenvironments are key components to successful cell culture and tissue engineering in vitro. One of the most accurate biomimetic microenvironments is that made by the cells themselves. Cell-made microenvironments are most similar to the in
Paula Benny, Michael Raghunath
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Seeing the Chemistry of Biomolecular Condensates: In Situ Mapping of Composition and Water Content
Advanced Science, EarlyView.Raman hyperspectral imaging combined with spectral phasor analysis enables a label‐free quantification of proteins, polymers and water density within individual biomolecular condensates. By transforming complex vibrational fingerprints into intuitive compositional maps, the approach reveals the high water content and structural heterogeneity of ...
E. Sabri +3 more
wiley +1 more source
Advanced Science, EarlyView.This review systematically elaborates on the mechanisms underlying physiological regulation and pathological dysregulation of biomolecular phase separation. It also summarizes advances in phase‑separation‑targeted therapeutic strategies and the design of functional biomaterials inspired by this process.
Jiawei Zhu +5 more
wiley +1 more source