Results 81 to 90 of about 103,280 (234)

Compression of random coils due to macromolecular crowding: Scaling effects [PDF]

, 2010
The addition of a macromolecular crowding agent to a dilute solution of polymer exerts a compressive force that tends to reduce the size of the chain. We study here the effect of changing the size ratio between the random coil and the crowding agent. The
P. Busch, J. Teixeira, Le Coeur, C., Longeville, S., Teixeira, J., C. Le Coeur, S. Longeville, Busch, P.   +7 more
core   +1 more source

Multifactorial bioengineering in the tendon context to maintain tenocyte phenotype and to direct dermal fibroblasts towards tenogenic lineage

Bioactive Materials
Considering that cells in vivo are simultaneously exposed to multiple signals, multifactorial bioengineering slowly but surely becomes the state of play in controlling cell function in vitro. In this context, herein we assessed the simultaneous effect of
Adrian Djalali-Cuevas, Mandy Rettel, Frank Stein, Mikhail Savitski, Ioannis Gkiatas, Anastasios Korompilias, Ioannis Skoufos, Athina Tzora, Nikitas Prassinos, Nikolaos Diakakis, Dimitrios I. Zeugolis   +10 more
doaj   +1 more source

Sticker Valence Governs Chain Collapse and Liquid‐Liquid Phase Separation in Peptide‐Inspired Associative Polymers

Advanced Science, EarlyView.
Peptide‐inspired associative polymers with arginine‐like guanidinium stickers and lysine‐like ammonium spacers undergo chain collapse and liquid‐liquid phase separation induced by reversible sticker associations. Sticker valence and tunable association strength jointly control the phase behavior, chain conformation, and interfacial cohesion of these ...
Seung‐Hwan Oh, Jinhoon Lee, Seunghyun Lee, Raehyun Jung, Sangyeop Lee, Su‐Mi Hur, Dong Woog Lee, Soo‐Hyung Choi   +7 more
wiley   +1 more source

Hydration‐Gated Photoinduced Electron Transfer in BODIPY Dyes

Angewandte Chemie, EarlyView.
Hydration switches photoinduced electron transfer (PET) ON in alkoxy‐coordinated BODIPY dyes via specific hydrogen bonding. This gating yields a fluorescence lifetime span of ∼6 ns, enabling fluorescence lifetime imaging microscopy (FLIM) contrast in live cells and organoids. ABSTRACT Fluorescent probes based on photoinduced electron transfer (PET) are
Venkata N. K. Mamillapalli, Irina A. Okkelman, Fatima Akhssas, Greta Sambucari, Angela C. Debruyne, Dinesh Beniwal, Christophe Bucher, Mariangela Di Donato, Ruslan I. Dmitriev, Mikhail A. Filatov   +9 more
wiley   +2 more sources

The unfolding of iRFP713 in a crowded milieu [PDF]

PeerJ, 2019
The exploring of biological processes in vitro under conditions of macromolecular crowding is a way to achieve an understanding of how these processes occur in vivo.
Olesya V. Stepanenko, Olga V. Stepanenko, Irina M. Kuznetsova, Konstantin K. Turoverov   +3 more
doaj   +2 more sources

RNA Promotes Synapsin Condensates That Organize Synaptic Vesicles in Live Neurons and Enable Localized Translation in Reconstituted System

Advanced Science, EarlyView.
Condensates at synapses organize synaptic vesicles (SVs) and are crucial for efficient neurotransmitter release, yet how RNA contributes to this mesoscale architecture remains poorly understood. Here we uncover that RNA modulates synapsin–driven condensates.
Branislava Rankovic, Zachary M. Geisterfer, Akshita Chhabra, Vladimir M. Jovanovic, Ian Seim, Christian Hoffmann, Antonela Condric, Gerard Aguilar Pérez, Rinse de Boer, Lars T. Hofstede, Jingao Sun, Kiara Freitag, Katja Nowick, Marina Jendrach, Frank L. Heppner, Vladimir Despic, Michaela Müller‐McNicoll, Stephan Sigrist, Geert van den Bogaart, Sofiia Reshetniak, Amy S. Gladfelter, Dragomir Milovanovic   +21 more
wiley   +1 more source

Macromolecular crowding effects in crowded biomolecular environments

, 2018
In dieser Arbeit werden Computer-Simulationen eingesetzt um den Einfluss biologisch relevanter, konzentrierter Umgebungen auf das strukturelle Gleichgewicht von (Bio-)Polymeren zu untersuchen. Für diese Simulationen wird ein zuvor beschriebener Monte Carlo Algorithmus verwendet, welcher die Flexibilität der Moleküle anhand von strukturellen Ensembles ...
openaire   +3 more sources

Crowding-facilitated macromolecular transport in attractive micropost arrays

Scientific Reports, 2017
Our study of DNA dynamics in weakly attractive nanofabricated post arrays revealed crowding enhances polymer transport, contrary to hindered transport in repulsive medium.
Fan-Tso Chien, Po-Keng Lin, Wei Chien, Cheng-Hsiang Hung, Ming-Hung Yu, Chia-Fu Chou, Yeng-Long Chen   +6 more
doaj   +1 more source

Brownian Dynamics Computational Model of Protein Diffusion in Crowded Media with Dextran Macromolecules as Obstacles

Entropy, 2017
The high concentration of macromolecules (i.e., macromolecular crowding) in cellular environments leads to large quantitative effects on the dynamic and equilibrium biological properties.
Pablo M. Blanco, Mireia Via, Josep Lluís Garcés, Sergio Madurga, Francesc Mas   +4 more
doaj   +1 more source

Antibody binding reports spatial heterogeneities in cell membrane organization

Nature Communications, 2023
The spatial organization of cell membrane glycoproteins and glycolipids is critical for mediating the binding of ligands, receptors, and macromolecules on the plasma membrane.
Daniel P. Arnold, Yaxin Xu, Sho C. Takatori   +2 more
doaj   +1 more source