Results 61 to 70 of about 228,358 (272)

In vivo macromolecular crowding is differentially modulated by aquaporin 0 in zebrafish lens: Insights from a nanoenvironment sensor and spectral imaging

Science Advances, 2022
Macromolecular crowding is crucial for cellular homeostasis. In vivo studies of macromolecular crowding and water dynamics are needed to understand their roles in cellular physiology and fate determination.
I. Vorontsova, Alexander Vallmitjana, Belén Torrado, T. Schilling, James E. Hall, Enrico Gratton, Leonel Malacrida   +6 more
semanticscholar   +1 more source

Molecular Basis for Actin Polymerization Kinetics Modulated by Solution Crowding

Biomolecules, 2023
Actin polymerization drives cell movement and provides cells with structural integrity. Intracellular environments contain high concentrations of solutes, including organic compounds, macromolecules, and proteins.
Bryan Demosthene, Myeongsang Lee, Ryan R. Marracino, James B. Heidings, Ellen Hyeran Kang   +4 more
doaj   +1 more source

Macromolecular Crowding Supports the Generation of Basal Membrane-Rich Pericyte-Based Cell Sheets Useful for Cell Therapy of Diabetic Wounds. [PDF]

Cell Transplant
Rampin A, Caravaggi CMF, Troisi L, Spinetti G.   +3 more
europepmc   +2 more sources

Macromolecular crowding transforms regenerative medicine by enabling the accelerated development of functional and truly three-dimensional cell assembled micro tissues.

Biomaterials, 2022
Scaffold-free in vitro organogenesis exploits the innate ability of cells to synthesise and deposit their own extracellular matrix to fabricate tissue-like assemblies.
A. De Pieri, S. Korntner, H. Capella-Monsonís, D. Tsiapalis, S. Kostjuk, S. Churbanov, P. Timashev, A. Gorelov, Y. Rochev, D. Zeugolis   +9 more
semanticscholar   +1 more source

Effects of Macromolecular Crowding on the Collapse of Biopolymers

Physical Review Letters, 2015
Experiments show that macromolecular crowding modestly reduces the size of intrinsically disordered proteins (IDPs) even at volume fraction ($ϕ$) similar to that in the cytosol whereas DNA undergoes a coil-to-globule transition at very small $ϕ$. We show using a combination of scaling arguments and simulations that the polymer size $\overline{R}_g(ϕ ...
Kang, Hongsuk, Pincus, Philip A., Hyeon, Changbong, Thirumalai, D.   +3 more
openaire   +4 more sources

Macromolecular crowding modulates α-synuclein amyloid fiber growth

Biophysical Journal, 2021
The crowdedness of living cells, hundreds of milligrams per milliliter of macromolecules, may affect protein folding, function, and misfolding. Still, such processes are most often studied in dilute solutions in vitro.
I. Horváth, Ranjeet Kumar, P. Wittung-Stafshede   +2 more
semanticscholar   +1 more source

Cells recognize osmotic stress through liquid–liquid phase separation lubricated with poly(ADP-ribose)

Nature Communications, 2021
Cells experience various osmotic perturbation, but cellular osmosensing mechanisms remain obscure. Here, the authors report that cells recognize osmotic stress from the inside through macromolecular crowding-driven and poly(ADP-ribose)-conditioned liquid–
Kengo Watanabe, Kazuhiro Morishita, Xiangyu Zhou, Shigeru Shiizaki, Yasuo Uchiyama, Masato Koike, Isao Naguro, Hidenori Ichijo   +7 more
doaj   +1 more source

Protein folding by the effects of macromolecular crowding [PDF]

Protein Science, 2004
Abstract Unfolded states of ribonuclease A were used to investigate the effects of macromolecular crowding on macromolecular compactness and protein folding. The extent of protein folding and compactness were measured by circular dichroism spectroscopy, fluorescence correlation spectroscopy, and NMR spectroscopy in the presence of ...
Nobuhiko, Tokuriki, Masataka, Kinjo, Shigeru, Negi, Masaru, Hoshino, Yuji, Goto, Itaru, Urabe, Tetsuya, Yomo   +6 more
openaire   +2 more sources

Macromolecular crowding and bicarbonate enhance the hydrogen peroxide-induced inactivation of glyceraldehyde-3-phosphate dehydrogenase. [PDF]

Biochem J
Bloemen RHJ, Radi R, Davies MJ, Fuentes-Lemus E.   +3 more
europepmc   +3 more sources

Transforming eukaryotic cell culture with macromolecular crowding.

TIBS -Trends in Biochemical Sciences. Regular ed, 2021
In multicellular organisms, the intracellular and extracellular spaces are considerably packed with a diverse range of macromolecular species. Yet, standard eukaryotic cell culture is performed in dilute, and deprived of macromolecules culture media ...
M. Raghunath, D. Zeugolis
semanticscholar   +1 more source