Results 101 to 110 of about 228,358 (272)

A Sensor for Quantification of Macromolecular Crowding in Living Cells [PDF]

Biophysical Journal, 2015
Macromolecular crowding in cells influences processes such as folding, association and diffusion of proteins and polynucleic acids. Direct spatiotemporal readout of crowding would be a powerful approach for unraveling the structure of the cytoplasm and determining the impact of excluded volume on protein function in living cells.
Boersma, Arnold J., Zuhorn, Inge S., Poolman, Bert   +2 more
openaire   +3 more sources

Multifactorial bioengineering in the tendon context to maintain tenocyte phenotype and to direct dermal fibroblasts towards tenogenic lineage

Bioactive Materials
Considering that cells in vivo are simultaneously exposed to multiple signals, multifactorial bioengineering slowly but surely becomes the state of play in controlling cell function in vitro. In this context, herein we assessed the simultaneous effect of
Adrian Djalali-Cuevas, Mandy Rettel, Frank Stein, Mikhail Savitski, Ioannis Gkiatas, Anastasios Korompilias, Ioannis Skoufos, Athina Tzora, Nikitas Prassinos, Nikolaos Diakakis, Dimitrios I. Zeugolis   +10 more
doaj   +1 more source

Harnessing Phase Separation for the Development of High‐Performance Hydrogels

Advanced Science, EarlyView.
ABSTRACT Hydrogels are indispensable for the development of next‐generation bioelectronics, soft robotics, and biomedical devices, where their mechanical properties determine performance and reliability. Among strategies to enhance hydrogel mechanics, phase separation enables controlled heterogeneity resulting in gel networks that are reinforced by ...
Yue Shao, Yiming Ma, Baihao Shao, Molly M. Stevens   +3 more
wiley   +1 more source

Click'n ROPISA: One‐Pot Synthesis of Ultrabright Fluorescent Nanoparticles Made of Polypeptides

Angewandte Chemie, EarlyView.
Self‐assembly and fluorophore conjugation proceed rapidly in a one‐pot “Click'n ROPISA” process, yielding intensely fluorescent polypeptide nanoparticles with tunable visible emission. High dye loading enables efficient energy transfer and single‐particle visualization, while the straightforward synthesis provides versatile access to bright ...
Amin El Jarroudi Hammou, Juliette Lavainne, Rohit Mangalwedhekar, Jyothis Devasia, Laurent Cognet, Sébastien Lecommandoux, Colin Bonduelle, Chloé Grazon   +7 more
wiley   +2 more sources

The unfolding of iRFP713 in a crowded milieu [PDF]

PeerJ, 2019
The exploring of biological processes in vitro under conditions of macromolecular crowding is a way to achieve an understanding of how these processes occur in vivo.
Olesya V. Stepanenko, Olga V. Stepanenko, Irina M. Kuznetsova, Konstantin K. Turoverov   +3 more
doaj   +2 more sources

Development of Biomimetic Substrates for Limbal Epithelial Stem Cells Using Collagen-Based Films, Hyaluronic Acid, Immortalized Cells, and Macromolecular Crowding. [PDF]

Life (Basel)
Gurdal M, Ercan G, Barut Selver O, Aberdam D, Zeugolis DI.   +4 more
europepmc   +2 more sources

Multi‐Stimuli Responsive Magneto‐Coacervate Droplets for Selective Molecular Enrichment and Programmable Manipulation

Advanced Science, EarlyView.
ABSTRACT Coacervate droplets formed via liquid–liquid phase separation offer unique opportunities as microreactors and delivery vehicles due to their ability to selectively concentrate biomolecules and support biochemical reactions. However, their passive nature severely restricts precise spatial and temporal control, posing barriers to practical ...
Kailang Liu, Haocheng Ran, Haohui Ou, Peiying Chen, Cheng Qi, Tiantian Kong, Zhou Liu   +6 more
wiley   +1 more source

Impact of Threading State on the Mechanics of γ‐Cyclodextrin–Pyrene Gels

Angewandte Chemie, EarlyView.
Tuning the threading state of discrete γ‐cyclodextrin (γ‐CD)–pyrene pseudorotaxane crosslinkers controls gel topology and ring mobility. The resulting doubly threaded slide‐ring networks exhibit solvent‐switchable dissipative dynamics, high extensibility, rate‐dependent mechanics, and low hysteresis, revealing how crosslink topology governs macroscopic
Jongwon Oh, Heyi Liang, Natsumi Nitta, Jongmin Kim, Yuan Tian, Guancen Liu, Jerald E. Hertzog, Juan J. de Pablo, Stuart J. Rowan   +8 more
wiley   +2 more sources

Crowding-facilitated macromolecular transport in attractive micropost arrays

Scientific Reports, 2017
Our study of DNA dynamics in weakly attractive nanofabricated post arrays revealed crowding enhances polymer transport, contrary to hindered transport in repulsive medium.
Fan-Tso Chien, Po-Keng Lin, Wei Chien, Cheng-Hsiang Hung, Ming-Hung Yu, Chia-Fu Chou, Yeng-Long Chen   +6 more
doaj   +1 more source

Brownian Dynamics Computational Model of Protein Diffusion in Crowded Media with Dextran Macromolecules as Obstacles

Entropy, 2017
The high concentration of macromolecules (i.e., macromolecular crowding) in cellular environments leads to large quantitative effects on the dynamic and equilibrium biological properties.
Pablo M. Blanco, Mireia Via, Josep Lluís Garcés, Sergio Madurga, Francesc Mas   +4 more
doaj   +1 more source