Results 11 to 20 of about 16,667 (258)

Making microenvironments: A look into incorporating macromolecular crowding into in vitro experiments, to generate biomimetic microenvironments which are capable of directing cell function for tissue engineering applications [PDF]

Journal of Tissue Engineering, 2017
Biomimetic microenvironments are key components to successful cell culture and tissue engineering in vitro. One of the most accurate biomimetic microenvironments is that made by the cells themselves. Cell-made microenvironments are most similar to the in
Paula Benny, Michael Raghunath
doaj   +2 more sources

An upper limit for macromolecular crowding effects [PDF]

BMC Biophysics, 2011
Abstract Background Solutions containing high macromolecule concentrations are predicted to affect a number of protein properties compared to those properties in dilute solution. In cells, these macromolecular crowders have a large range of sizes and can occupy 30% or more of the available volume.
Andrew C. Miklos, Conggang Li, Courtney D. Sorrell, L. Andrew Lyon, Gary J. Pielak   +4 more
openalex   +5 more sources

Brownian Dynamics Computational Model of Protein Diffusion in Crowded Media with Dextran Macromolecules as Obstacles [PDF]

Entropy, 2017
The high concentration of macromolecules (i.e., macromolecular crowding) in cellular environments leads to large quantitative effects on the dynamic and equilibrium biological properties.
Pablo M. Blanco, Mireia Via, Josep Lluís Garcés, Sergio Madurga, Francesc Mas   +4 more
doaj   +6 more sources

PEG-mCherry interactions beyond classical macromolecular crowding. [PDF]

Protein Sci
AbstractThe dense cellular environment influences bio-macromolecular structure, dynamics, interactions and function. Despite advancements in understanding protein-crowder interactions, predicting their precise effects on protein structure and function remains challenging. Here, we elucidate the effects of PEG-induced crowding on the fluorescent protein
Haas-Neill L, Joron K, Lerner E, Rauscher S.   +3 more
europepmc   +3 more sources

Stress-dependent macromolecular crowding in the mitochondrial matrix. [PDF]

EMBO J, 2023
AbstractMacromolecules of various sizes induce crowding of the cellular environment. This crowding impacts on biochemical reactions by increasing solvent viscosity, decreasing the water‐accessible volume and altering protein shape, function, and interactions.
Bulthuis EP, Dieteren CEJ, Bergmans J, Berkhout J, Wagenaars JA, van de Westerlo EMA, Podhumljak E, Hink MA, Hesp LFB, Rosa HS, Malik AN, Lindert MK, Willems PHGM, Gardeniers HJGE, den Otter WK, Adjobo-Hermans MJW, Koopman WJH.   +16 more
europepmc   +7 more sources

High Macromolecular Crowding in Liposomes from Microfluidics. [PDF]

Adv Sci (Weinh), 2022
AbstractThe intracellular environment is crowded with macromolecules that influence biochemical equilibria and biomacromolecule diffusion. The incorporation of such crowding in synthetic cells would be needed to mimic the biochemistry of living cells.
Guerzoni LPB, de Goes AVC, Kalacheva M, Haduła J, Mork M, De Laporte L, Boersma AJ.   +6 more
europepmc   +5 more sources

Macromolecular Crowding Modulates Cross-Bridge Performance [PDF]

Biophysical Journal, 2015
Actomyosin kinetics is usually studied in dilute solutions, which do not reflect conditions in the cytoplasm. In cells, myosin and actin work in a dense macromolecular environment. High concentrations of macromolecules dramatically reduce the amount of free space available for all solutes, which results in an effective increase of the solutes' chemical
Jinghua Ge, Sherry D. Bouriyaphone, Yuri E. Nesmelov   +2 more
openalex   +4 more sources

Amplifying the Macromolecular Crowding Effect Using Nanoparticles [PDF]

Journal of the American Chemical Society, 2012
This document is the Accepted Manuscript version of a Published Work that appeared in final form in Journal of the American Chemistry Society copyright © American Chemical Society after peer review and technical editing by publisher.
Dave, Neeshma, Liu, Juewen, Zaki, Ahmed
core   +5 more sources

In vitro and in vivo assessment of a non-animal sourced chitosan scaffold loaded with xeno-free umbilical cord mesenchymal stromal cells cultured under macromolecular crowding conditions [PDF]

Biomaterials and Biosystems
There is an increasing demand to not only accelerate the development of advanced therapy tissue engineered medicines, but to also eliminate xenogeneic materials from their development cycle.
Alessia Di Nubila, Meletios-Nikolaos Doulgkeroglou, Mehmet Gurdal, Stefanie H. Korntner, Dimitrios I. Zeugolis   +4 more
doaj   +2 more sources

Macromolecular crowding and supersaturation protect hemodialysis patients from the onset of dialysis-related amyloidosis [PDF]

Nature Communications, 2022
Amyloid fibrils of β2-microglobulin (β2m) can cause dialysis-related amyloidosis. Here, the authors show that a decrease in serum albumin levels in long-term dialysis deteriorates the inhibitory effects of serum milieux on supersaturation-limited amyloid
Kichitaro Nakajima, Keiichi Yamaguchi, Masahiro Noji, César Aguirre, Kensuke Ikenaka, Hideki Mochizuki, Lianjie Zhou, Hirotsugu Ogi, Toru Ito, Ichiei Narita, Fumitake Gejyo, Hironobu Naiki, Suguru Yamamoto, Yuji Goto   +13 more
doaj   +2 more sources