Results 51 to 60 of about 16,667 (258)
Impact of macromolecular crowding on DNA replication [PDF]
, 2014 Enzymatic activities in vivo occur in a crowded environment composed of many macromolecules. This environment influences DNA replication by increasing the concentration of the constituents, desolvation, decreasing the degrees of freedom for diffusion and
Akabayov, Barak +4 more
core +1 more source
Molecular Basis for Actin Polymerization Kinetics Modulated by Solution Crowding
Biomolecules, 2023 Actin polymerization drives cell movement and provides cells with structural integrity. Intracellular environments contain high concentrations of solutes, including organic compounds, macromolecules, and proteins.
Bryan Demosthene +4 more
doaj +1 more source
Optimization of ClpXP activity and protein synthesis in an E. coli extract-based cell-free expression system. [PDF]
, 2018 Protein degradation is a fundamental process in all living cells and is essential to remove both damaged proteins and intact proteins that are no longer needed by the cell.
Joseph, Simpson +4 more
core +2 more sources
, 2016 {\alpha}-synuclein ({\alpha}-syn) is an intrinsically disordered protein which is considered to be one of the causes of Parkinson's disease. This protein forms amyloid fibrils when in a highly concentrated solution.
Kikuchi, Macoto, Shirai, Nobu C.
core +1 more source
Synthetic Biology: A Bridge between Artificial and Natural Cells. [PDF]
, 2014 Artificial cells are simple cell-like entities that possess certain properties of natural cells. In general, artificial cells are constructed using three parts: (1) biological membranes that serve as protective barriers, while allowing communication ...
Ding, Yunfeng, Tan, Cheemeng, Wu, Fan
core +5 more sources
Nature Communications, 2021 Cells experience various osmotic perturbation, but cellular osmosensing mechanisms remain obscure. Here, the authors report that cells recognize osmotic stress from the inside through macromolecular crowding-driven and poly(ADP-ribose)-conditioned liquid–
Kengo Watanabe +7 more
doaj +1 more source
Protein Diffusion and Macromolecular Crowding [PDF]
Biophysical Journal, 2010 Having recently quantified how crowding can affect equilibrium protein stability (1), we have turned our attention to diffusion. Our test molecule is the small globular protein, chymotrypsin inhibitor 2. Our crowding molecules are both the synthetic polymer polyvinylpyrrolidone (PVP) and several globular proteins.
Pielak, Gary +2 more
openaire +1 more source
Protein Stability and Macromolecular Crowding [PDF]
Biophysical Journal, 2012 The environment inside cells is vastly different from the dilute, idealized conditions used in nearly all biophysical studies. Cells are exceptionally complex and contain macromolecules at concentrations exceeding 300 g/L. There are at least two profound consequences of the cellular environment that impact globular proteins.
Sarkar, Mohona +3 more
openaire +1 more source
Macromolecular crowding induces holo α-lactalbumin aggregation by converting to its apo form.
PLoS ONE, 2014 Macromolecular crowding has been shown to have an exacerbating effect on the aggregation propensity of amyloidogenic proteins; while having an inhibitory effect on the non-amyloidogenic proteins.
Shruti Mittal +1 more
doaj +1 more source
Effects of macromolecular crowding on protein conformational changes. [PDF]
PLoS Computational Biology, 2010 Many protein functions can be directly linked to conformational changes. Inside cells, the equilibria and transition rates between different conformations may be affected by macromolecular crowding.
Hao Dong, Sanbo Qin, Huan-Xiang Zhou
doaj +1 more source