Results 71 to 80 of about 16,667 (258)

Enzyme activity in the crowded milieu.

PLoS ONE, 2012
The cytosol of a cell is a concentrated milieu of a variety of different molecules, including small molecules (salts and metabolites) and macromolecules such as nucleic acids, polysaccharides, proteins and large macromolecular complexes.
Tobias Vöpel, George I Makhatadze
doaj   +1 more source

Progress on Crowding Effect in Cell-like Structures

Membranes, 2022
Several biological macromolecules, such as proteins, nucleic acids, and polysaccharides, occupy about 30% of the space in cells, resulting in a crowded macromolecule environment.
Chao Li, Xiangxiang Zhang, Mingdong Dong, Xiaojun Han   +3 more
doaj   +1 more source

Microfluidic Platform for Multiparametric Profiling of Fibrin Permeability, Fibrinolysis, and Cell Invasion

Advanced Functional Materials, EarlyView.
This paper introduces a single‐channel H‐junction microfluidic assay that profiles fibrin's evolving function in repair and thrombosis by measuring, in one ∼3 µL gel, permeability, fibrinolysis kinetics, fibroblast invasion, and clot extension in real time.
Halston Deal, Elizabeth M. Byrnes, Sanika Pandit, Halle J. Cantor, Muskan Aslam, Stacy Ban, Megan Palmisano, Rosemary Bayless, Ashley C. Brown, Michael Daniele   +9 more
wiley   +1 more source

Large-scale analysis of macromolecular crowding effects on protein aggregation using a reconstituted cell-free translation system

Frontiers in Microbiology, 2015
Proteins must fold into their native structures in the crowded cellular environment, to perform their functions. Although such macromolecular crowding has been considered to affect the folding properties of proteins, large-scale experimental data have so
Tatsuya eNiwa, Ryota eSugimoto, Lisa eWatanabe, Shugo eNakamura, Takuya eUeda, Hideki eTaguchi   +5 more
doaj   +1 more source

Macromolecular crowding as a suppressor of human IAPP fibril formation and cytotoxicity. [PDF]

PLoS ONE, 2013
The biological cell is known to exhibit a highly crowded milieu, which significantly influences protein aggregation and association processes. As several cell degenerative diseases are related to the self-association and fibrillation of amyloidogenic ...
Janine Seeliger, Alexander Werkmüller, Roland Winter   +2 more
doaj   +1 more source

Role of dynamic capsomere supply for viral capsid self-assembly

, 2015
Many viruses rely on the self-assembly of their capsids to protect and transport their genomic material. For many viral systems, in particular for human viruses like hepatitis B, adeno or human immunodeficiency virus, that lead to persistent infections ...
Boettcher, Marvin A., Klein, Heinrich C. R., Schwarz, Ulrich S.   +2 more
core   +1 more source

Quantitative constraint-based computational model of tumor-to-stroma coupling via lactate shuttle [PDF]

, 2015
Cancer cells utilize large amounts of ATP to sustain growth, relying primarily on non-oxidative, fermentative pathways for its production.
CAPUANI, FABRIZIO, DE MARTINO, ANDREA, DE MARTINO, DANIELE, MARINARI, Vincenzo   +3 more
core   +1 more source

Impact of macromolecular crowding on DNA replication [PDF]

Nature Communications, 2013
Enzymatic activities in vivo occur in a crowded environment composed of many macromolecules. This environment influences DNA replication by increasing the concentration of the constituents, desolvation, decreasing the degrees of freedom for diffusion and hopping of proteins onto DNA, and enhancing binding equilibria and catalysis.
Akabayov, Barak, Akabayov, Sabine R., Wagner, Gerhard, Richardson, Charles, Lee, Seung–Joo   +4 more
openaire   +3 more sources

The Terminal Domains of Collagen‐Like Silk Direct Molecular Interactions and Liquid–Liquid Phase Separation‐Mediated Material Assembly

Advanced Functional Materials, EarlyView.
Terminal domains of recombinant collagen‐like silk regulate hierarchical self‐assembly across molecular and material scales. We show that terminal domains of collagen‐like silk enhance triple‐helix stability and drive liquid–liquid phase separation, enabling reversible formation of fibers and self‐healing films.
Mengjie Shen, Daniil Astapov, Nur Alia Oktaviani, Mami Goto, Keiji Numata, A. Sesilja Aranko   +5 more
wiley   +1 more source

The 2D Structure of the T. brucei Preedited RPS12 mRNA Is Not Affected by Macromolecular Crowding

Journal of Nucleic Acids, 2017
Mitochondrial transcript maturation in African trypanosomes requires RNA editing to convert sequence-deficient pre-mRNAs into translatable mRNAs. The different pre-mRNAs have been shown to adopt highly stable 2D folds; however, it is not known whether ...
W.-Matthias Leeder, Stephan Voskuhl, H. Ulrich Göringer   +2 more
doaj   +1 more source