Results 21 to 30 of about 20,289 (228)

Endocytosis in filter-grown Madin-Darby canine kidney cells. [PDF]

open access: yesThe Journal of cell biology, 1989
In this paper, we have characterized the apical and basolateral endocytic pathways of epithelial MDCK cells grown on filters. The three-dimensional organization of the endocytic compartments was analyzed by confocal microscopy after internalization of a fluorescent fluid-phase marker from either side of the cell layer.
Bomsel, M.   +4 more
openaire   +3 more sources

Sphingomyelin metabolism is involved in the differentiation of MDCK cells induced by environmental hypertonicity

open access: yesJournal of Lipid Research, 2015
Sphingolipids (SLs) are relevant lipid components of eukaryotic cells. Besides regulating various cellular processes, SLs provide the structural framework for plasma membrane organization.
Nicolás Octavio Favale   +4 more
doaj   +1 more source

Substrate Stiffness Determines the Establishment of Apical-Basal Polarization in Renal Epithelial Cells but Not in Tubuloid-Derived Cells

open access: yesFrontiers in Bioengineering and Biotechnology, 2022
Mechanical guidance of tissue morphogenesis is an emerging method of regenerative medicine that can be employed to steer functional kidney architecture for the purpose of bioartificial kidney design or renal tissue engineering strategies.
Maria J. Hagelaars   +9 more
doaj   +1 more source

Lixazinone Stimulates Mitogenesis of Madin-Darby Canine Kidney Cells

open access: yesExperimental Biology and Medicine, 2006
Polycystic kidney diseases (PKD) are characterized by excessive proliferation of renal tubular epithelial cells, development of fluid-filled cysts, and progressive renal insufficiency. cAMP inhibits proliferation of normal renal tubular epithelial cells but stimulates proliferation of renal tubular epithelial cells derived from patients with PKD. Madin-
Jingfei, Cheng   +6 more
openaire   +2 more sources

Involvement of Intracellular Ca2+ in the Regulatory Volume Decrease After Hyposmotic Swelling in MDCK Cells

open access: yesJournal of Pharmacological Sciences, 2007
We examined the source of Ca2+ involved in the volume regulation of Madin-Darby canine kidney (MDCK) cells with confocal microscopy and fluoroprobes. Hyposmosis induced a transient increase in cell volume, as well as cytoplasmic Ca2+, which peaked at 3 ...
Naoko Iida-Tanaka   +7 more
doaj   +1 more source

A conserved LDL-receptor motif regulates corin and CD320 membrane targeting in polarized renal epithelial cells

open access: yeseLife, 2020
Selective protein distribution on distinct plasma membranes is important for epithelial cell function. To date, how proteins are directed to specific epithelial cell surface is not fully understood. Here we report a conserved DSSDE motif in LDL-receptor (
Ce Zhang   +9 more
doaj   +1 more source

NaCl transport by Madin Darby canine kidney cyst epithelial cells

open access: yesKidney International, 1992
The mechanism of NaCl transport across the epithelium of intact MDCK cysts grown in a collagen gel matrix was investigated. Double-barreled microelectrodes were used to measure basolateral membrane PD (Vbl), transepithelial PD (Vt), and intracellular (Cli) and intralumenal (Clcy) Cl- activities in cysts under different conditions. In a control Ringer's
Macias, William L.   +4 more
openaire   +2 more sources

Regulation of epithelial migration by epithelial cell adhesion molecule requires its Claudin-7 interaction domain. [PDF]

open access: yesPLoS ONE, 2018
Epithelial cell adhesion molecule (EpCAM) is a glycoprotein on the surface of epithelial cells that is essential for intestinal epithelial integrity and expressed at high levels in many epithelial derived cancers and circulating tumor cells. Here we show
Angela I M Barth   +2 more
doaj   +1 more source

Sonic hedgehog is basolaterally sorted from the TGN and transcytosed to the apical domain involving Dispatched-1 at Rab11-ARE

open access: yesFrontiers in Cell and Developmental Biology, 2022
Hedgehog proteins (Hhs) secretion from apical and/or basolateral domains occurs in different epithelial cells impacting development and tissue homeostasis.
Lisette Sandoval   +9 more
doaj   +1 more source

cfa-miR-143 Promotes Apoptosis via the p53 Pathway in Canine Influenza Virus H3N2-Infected Cells

open access: yesViruses, 2017
MicroRNAs regulate multiple aspects of the host response to viral infection. This study verified that the expression of cfa-miR-143 was upregulated in vivo and in vitro by canine influenza virus (CIV) H3N2 infection. To understand the role of cfa-miR-143
Pei Zhou   +9 more
doaj   +1 more source

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