Results 151 to 160 of about 11,749,169 (183)

The Eukaryotic Mcm2-7 Replicative Helicase

2012
In eukaryotes, the Mcm2-7 complex forms the core of the replicative helicase - the molecular motor that uses ATP binding and hydrolysis to fuel the unwinding of double-stranded DNA at the replication fork. Although it is a toroidal hexameric helicase superficially resembling better-studied homohexameric helicases from prokaryotes and viruses, Mcm2-7 is
Sriram, Vijayraghavan, Anthony, Schwacha
openaire   +2 more sources

Dynamic loading and redistribution of the Mcm2‐7 helicase complex through the cell cycle [PDF]

EMBO Journal, 2015
Eukaryotic replication origins are defined by the ORC-dependent loading of the Mcm2-7 helicase complex onto chromatin in G1. Paradoxically, there is a vast excess of Mcm2-7 relative to ORC assembled onto chromatin in G1. These excess Mcm2-7 complexes exhibit little co-localization with ORC or replication foci and can function as dormant origins.
Heather K Macalpine, Yulong Li, Jason A Belsky   +2 more
exaly   +3 more sources

Interaction of human MCM2-7 proteins with TIM, TIPIN and Rb

The Journal of Biochemistry, 2010
Interactions of human MCM2-7 proteins with the proteins of TIM, TIPIN, an amino-terminal fragment of Rb, and p27 were examined by co-immuno-precipitation experiment using cell lysates of co-expressed insect cells. TIM and TIPIN, both of which are involved in regulation of DNA replication fork progression, mainly interacted with MCM3-7 proteins.
Yuki, Numata, Shouta, Ishihara, Naoko, Hasegawa, Naohito, Nozaki, Yukio, Ishimi   +4 more
openaire   +2 more sources

Pumps, paradoxes and ploughshares: mechanism of the MCM2–7 DNA helicase

Trends in Biochemical Sciences, 2005
In eukaryotes, numerous lines of evidence have coalesced into a convincing case that the MCM2-7 complex - a heterohexameric ATPase - is the replicative DNA helicase. However, almost nothing is known about how this enzyme functions in a cellular context.
Tatsuro S, Takahashi, Dale B, Wigley, Johannes C, Walter   +2 more
openaire   +2 more sources

Uninterrupted MCM2-7 Function Required for DNA Replication Fork Progression

Science, 2000
Little is known about the DNA helicases required for the elongation phase of eukaryotic chromosome replication. Minichromosome maintenance (MCM) protein complexes have DNA helicase activity but have only been functionally implicated in initiating DNA replication. Using an improved method for constructing conditional degron mutants, we show
Labib, K, Tercero, JA, Diffley, JFX
openaire   +3 more sources

The role of the MCM2-7 helicase complex during Arabidopsis seed development

Plant Molecular Biology, 2014
The MINICHROMOSOME MAINTENANCE 2-7 (MCM2-7) complex, a ring-shaped heterohexamer, unwinds the DNA double helix ahead of the other replication machinery. Although there is evidence that individual components might have other roles, the essential nature of the MCM2-7 complex in DNA replication has made it difficult to uncover these.
Rowan P, Herridge, Robert C, Day, Richard C, Macknight   +2 more
openaire   +2 more sources

The Role of Mcm2–7 in Replication Initiation

2016
The hetero-hexameric Mcm2–7 complex is a multifunctional ATPase that plays essential roles during the initiation of DNA replication in eukaryotic cells. Initially, the Mcm2–7 complex is bound as a catalytically inactive double hexamer around double-stranded DNA, marking potential replication origin sites along the chromosome.
openaire   +1 more source

A Meier-Gorlin syndrome mutation impairs the loading of the MCM2-7 complex during DNA replication initiation.

Proceedings of the National Academy of Sciences of the United States of America
Chromatin loading of the hexameric replicative helicase MCM2-7 complex requires coordinated interactions with the origin recognition complex (ORC), CDC6, and CDT1.
Yusong Liu, Mengquan Yang, Ping Lu, Haishan Gao, Maozhou He, Yitao Wang, Ao Qi, Ting Cao, Qiuqin Zhang, Shutao Qi, Yigong Shi, Hongtao Yu   +11 more
semanticscholar   +1 more source

MCM2-7 proteins promote NF-κB transcriptional activity through cooperative promoter recruitment.

Bioscience Reports
The minichromosome maintenance 2-7 (MCM2-7) proteins form a hexameric protein complex that is the structural core of replicative helicase, which is essential for accurate DNA replication.
Xian Hong, Ming Sui, Qiang Li, Yan Lu, Y. Nie, Yongpei Li, Wen-Long Li, Xin-Xin Yue, Jing Xu, D. Billadeau, Tao Wang, Zhi-Hui Deng   +11 more
semanticscholar   +1 more source

Inhibition of DNA binding of MCM2-7 complex by phosphorylation with cyclin-dependent kinases

The Journal of Biochemistry, 2013
Cyclin-dependent kinase (CDK) that plays a central role in preventing re-replication of DNA phosphorylates several replication proteins to inactivate them. MCM4 in MCM2-7 and RPA2 in RPA are phosphorylated with CDK in vivo. There are inversed correlations between the phosphorylation of these proteins and their chromatin binding.
Mariko, Moritani, Yukio, Ishimi
openaire   +2 more sources