Results 1 to 10 of about 60,380 (203)

Comparative Susceptibility of Madin–Darby Canine Kidney (MDCK) Derived Cell Lines for Isolation of Swine Origin Influenza A Viruses from Different Clinical Specimens

Viruses, 2021
Madin–Darby canine kidney (MDCK) cells are commonly used for the isolation of mammalian influenza A viruses. The goal of this study was to compare the sensitivity and suitability of the original MDCK cell line in comparison with MDCK-derived cell lines ...
Yohannes Berhane
exaly   +3 more sources

Metabolomics profiling reveals differences in proliferation between tumorigenic and non-tumorigenic Madin-Darby canine kidney (MDCK) cells [PDF]

PeerJ, 2023
Background Madin-Darby canine kidney (MDCK) cells are a cellular matrix in the production of influenza vaccines. The proliferation rate of MDCK cells is one of the critical factors that determine the vaccine production cycle.
Na Sun, Yuchuan Zhang, Jian Dong, Geng Liu, Zhenbin Liu, Jiamin Wang, Zilin Qiao, Jiayou Zhang, Kai Duan, Xuanxuan Nian, Zhongren Ma, Xiaoming Yang   +11 more
doaj   +2 more sources

IRF7-deficient MDCK cell based on CRISPR/Cas9 technology for enhancing influenza virus replication and improving vaccine production [PDF]

PeerJ, 2022
The influenza virus is a cause of seasonal epidemic disease and enormous economic injury. The best way to control influenza outbreaks is through vaccination.
Oraphan Mayuramart, Witthaya Poomipak, Somruthai Rattanaburi, Kritsada Khongnomnan, Songtham Anuntakarun, Suthat Saengchoowong, Tanit Chavalit, Naphat Chantaravisoot, Sunchai Payungporn   +8 more
doaj   +2 more sources

Isolation of MDCK cells with low expression of mdr1 gene and their use in membrane permeability screening

Acta Pharmaceutica, 2022
The Madin-Darby canine kidney (MDCK) cell line is frequently used for permeability screening in drug discovery. It contains endogenous transporters, most prominently canine multidrug resistance P-glycoprotein (Mdr1), which can interfere with studies of P-
BOKULIĆ ANA, PADOVAN JASNA, STUPIN-POLANČEC DARIJA, MILIĆ ASTRID   +3 more
doaj   +1 more source

Comparison of Various Cell Lines and Three-Dimensional Mucociliary Tissue Model Systems to Estimate Drug Permeability Using an In Vitro Transport Study to Predict Nasal Drug Absorption in Rats

Pharmaceutics, 2020
Recently, various types of cultured cells have been used to research the mechanisms of transport and metabolism of drugs. Although many studies using cultured cell systems have been published, a comparison of different cultured cell systems has never ...
Tomoyuki Furubayashi, Daisuke Inoue, Noriko Nishiyama, Akiko Tanaka, Reiko Yutani, Shunsuke Kimura, Hidemasa Katsumi, Akira Yamamoto, Toshiyasu Sakane   +8 more
doaj   +1 more source

Establishment and Validation of a New Co-Culture for the Evaluation of the Permeability through the Blood–Brain Barrier in Patients with Glioblastoma

Pharmaceutics, 2023
Currently, the mechanisms involved in drug access to the central nervous system (CNS) are not completely elucidated, and research efforts to understand the behaviour of the therapeutic agents to access the blood–brain barrier continue with the utmost ...
Bárbara Sánchez-Dengra, Elena García-Montoya, Isabel González-Álvarez, Marival Bermejo, Marta González-Álvarez   +4 more
doaj   +1 more source

Baicalin Exhibits a Protective Effect against Cisplatin-Induced Cytotoxic Damage in Canine Renal Tubular Epithelial Cells

Metabolites, 2023
Renal failure is a common chronic disease in dogs that substantially affects both their quality of life and longevity. The objective of this study was to assess the protective mechanisms of baicalin in cisplatin-induced Madin–Darby canine kidney (MDCK ...
Yao Wang, Xiao Li, Chuanguo Yan, Liuwei Xie, Yang Yang   +4 more
doaj   +1 more source

TRAF3 deficiency in MDCK cells improved sensitivity to the influenza A virus

Heliyon, 2023
Tumor necrosis factor receptor-associated factor 3 (TRAF3), an adaptor protein, has significant and varying effects on immunity depending on cell types. The role of TRAF3 in Madin-Darby Canine Kidney Epithelial (MDCK) cell resistance to influenza A virus
Yang Le, Jiayou Zhang, Zheng Gong, Zhegang Zhang, Xuanxuan Nian, Xuedan Li, Daiguan Yu, Ning Ma, Rong Zhou, Guomei Zhang, Bo Liu, Lu Yang, Baiqi Fu, Xiuqin Xu, Xiaoming Yang   +14 more
doaj   +1 more source

Stable Ectopic Expression of ST6GALNAC5 Induces Autocrine MET Activation and Anchorage-Independence in MDCK Cells. [PDF]

PLoS ONE, 2016
The epithelial-mesenchymal transition (EMT) is a complex cancer progression that can boost the metastatic potential of transformed cells by inducing migration, loss of cell adhesion, and promoting proliferation under anchorage-independent conditions.
Chia Chu, Donald P Bottaro, Michael J Betenbaugh, Joseph Shiloach   +3 more
doaj   +1 more source

Alternative Experimental Models for Studying Influenza Proteins, Host–Virus Interactions and Anti-Influenza Drugs

Pharmaceuticals, 2019
Ninety years after the discovery of the virus causing the influenza disease, this malady remains one of the biggest public health threats to mankind. Currently available drugs and vaccines only partially reduce deaths and hospitalizations.
Sonja C. J. H. Chua, Hui Qing Tan, David Engelberg, Lina H. K. Lim   +3 more
doaj   +1 more source