Claudin-2 knockout by TALEN-mediated gene targeting in MDCK cells: claudin-2 independently determines the leaky property of tight junctions in MDCK cells. [PDF]
PLoS ONE, 2015 Tight junctions (TJs) regulate the movements of substances through the paracellular pathway, and claudins are major determinants of TJ permeability. Claudin-2 forms high conductive cation pores in TJs.
Shinsaku Tokuda, Mikio Furuse
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Functional evaluation for adequacy of MDCK-lineage cells in influenza research
BMC Research Notes, 2019 Objective Influenza is an acute respiratory disease caused by the influenza virus which circulates annually in populations of different species. Madin-Darby Canine Kidney (MDCK) is the most widely utilized cell-line for conducting influenza research ...
Hsin-Chung Tsai +4 more
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High yield production of influenza virus in Madin Darby canine kidney (MDCK) cells with stable knockdown of IRF7. [PDF]
PLoS ONE, 2013 Influenza is a serious public health problem that causes a contagious respiratory disease. Vaccination is the most effective strategy to reduce transmission and prevent influenza.
Itsuki Hamamoto +3 more
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Shear-induced Volume Decrease in MDCK Cells [PDF]
Cellular Physiology and Biochemistry, 2012 Using a microfluidic cell volume sensor we measured the change in the cell volume of Madin-Darby Canine Kidney (MDCK) cells induced by shear stress. An increase in shear stress from 0.2 to 2.0 dyn/cm(2) resulted in a volume decrease to a steady state volume ∼ 20 - 30 % smaller than the initial resting cell volume.
Jinseok, Heo +3 more
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Scientific Reports, 2018 Trimethylamine-N-oxide (TMAO) gained considerable attention because of its role as a cardiovascular risk biomarker. Organic cation transporter 2 (OCT2) mediates TMAO uptake into renal proximal tubular cells.
A. Gessner, J. König, M. F. Fromm
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Steviol reduces MDCK Cyst formation and growth by inhibiting CFTR channel activity and promoting proteasome-mediated CFTR degradation. [PDF]
PLoS ONE, 2013 Cyst enlargement in polycystic kidney disease (PKD) involves cAMP-activated proliferation of cyst-lining epithelial cells and transepithelial fluid secretion into the cyst lumen via cystic fibrosis transmembrane conductance regulator (CFTR) chloride ...
Chaowalit Yuajit +5 more
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Comparison of Human and Canine P-Glycoprotein Substrates at R, P, and H Binding Sites. [PDF]
J Vet Pharmacol TherABSTRACT P‐glycoprotein (P‐gp) greatly impacts substrate drug disposition, so much so that regulatory agencies recommend ascertaining the P‐gp status of active pharmaceutical ingredients (APIs) intended for human use. Arguably, the P‐gp status of drugs intended for canine patients is equally, if not more, important.
Burke NS, Mealey KL.
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Comparison of mutation patterns in full-genome A/H3N2 influenza sequences obtained directly from clinical samples and the same samples after a single MDCK passage. [PDF]
PLoS ONE, 2013 Human influenza viruses can be isolated efficiently from clinical samples using Madin-Darby canine kidney (MDCK) cells. However, this process is known to induce mutations in the virus as it adapts to this non-human cell-line.
Hong Kai Lee +6 more
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PrPC Undergoes Basal to Apical Transcytosis in Polarized Epithelial MDCK Cells. [PDF]
PLoS ONE, 2016 The Prion Protein (PrP) is an ubiquitously expressed glycosylated membrane protein attached to the external leaflet of the plasma membrane via a glycosylphosphatidylinositol anchor (GPI).
Alexander Arkhipenko +4 more
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Influenza entry pathways in polarized MDCK cells
Biochemical and Biophysical Research Communications, 2014 In non-polarized cell culture models, influenza virus has been shown to enter host cells via multiple endocytic pathways, including classical clathrin-mediated endocytic routes (CME), clathrin- and caveolae-independent routes and macropinocytosis. However, little is known about the entry route of influenza virus in differentiated epithelia, in vivo ...
Zhang, Yueting, Whittaker, Gary R.
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