Results 61 to 70 of about 57,512 (227)

An alphaherpesvirus exploits antimicrobial beta-defensins to initiate respiratory tract infection [PDF]

, 2020
beta-Defensins protect the respiratory tract against the myriad of microbial pathogens entering the airways with each breath. However, this potentially hostile environment is known to serve as a portal of entry for herpesviruses.
Boyen, Filip, Daled, Simon, Dhaenens, Maarten, Laval, Kathlyn, Nauwynck, Hans, Pasmans, Frank, Poelaert, Katrien, Van Cleemput, Jolien, Vanderheijden, Nathalie   +8 more
core   +2 more sources

A New 3D Colon on a Chip to Decipher the Influence of Mechanical Forces on the Physiological Cellular Ecosystem

Advanced Healthcare Materials, EarlyView.
To dissect how mechanical forces influence intestinal physiology, we developed a stretchable 3D colon‐on‐chip that integrates tunable topography, stiffness and peristalsis‐like motion within a physiologically relevant microenvironment. We showed that stretching is a dominant factor governing epithelial behavior, markedly enhancing proliferation and ...
Moencopi Bernheim‐Dennery, Lauriane Gérémie, Julie Brun, Lucas Chassatte, Giacomo Gropplero, Réda Bouras, Jieun Choo, Bertrand Cinquin, Alba Marcellan, Danijela Matic Vignjevic, Stéphanie Descroix   +10 more
wiley   +1 more source

Expression Profile of Drug and Nutrient Absorption Related Genes in Madin-Darby Canine Kidney (MDCK) Cells Grown under Differentiation Conditions

Pharmaceutics, 2012
The expression levels of genes involved in drug and nutrient absorption were evaluated in the Madin-Darby Canine Kidney (MDCK) <em>in vitro</em> drug absorption model.
Balvinder S. Vig, Ronald L. Smith, Teresa N. Faria, Doris A. Wall, Aiqing He, Charles A. Tilford, Yisheng Jin, Yong Quan   +7 more
doaj   +1 more source

Continuous growth of proximal tubular kidney epithelial cells in hormone-supplemented serum-free medium. [PDF]

, 1982
An epithelial cell line from pig kidney (LLC-PK1) with properties of proximal tubular cells can be maintained indefinitely in hormone-supplemented serum-free medium. Continuous growth requires the presence of seven factors: transferrin, insulin, selenium,
Chuman, L, Cohen, AH, Fine, LG, Saier, MH   +3 more
core  

International Laboratory Comparison of Influenza Microneutralization Assays for A(H1N1) pdm09, A(H3N2), and A(H5N1) Influenza Viruses by CONSISE [PDF]

, 2015
The microneutralization assay is commonly used to detect antibodies to influenza virus, and multiple protocols are used worldwide. These protocols differ in the incubation time of the assay as well as in the order of specific steps, and even within ...
Engelhardt, OG, Heath, A, Hoschler, K, Hungnes, O, Katz, JM, Laurie, KL, Peiris, M, Van Kerkhove, MD, Wood, J, Zhang, W   +9 more
core   +1 more source

Attenuation of Influenza a Virus into Live Vaccines Through C‐End Degrons

Advanced Science, EarlyView.
Naturally occurring C‐end degrons are exploited to design proteolysis‐targeting live attenuated influenza vaccines. C‐end degron tagging of viral M1 protein promotes proteasome‐dependent degradation, resulting in robust attenuation in host cells while permitting scalable production in engineered cells.
Ping Wang, Le Li, Yunfang Chen, Le Tong, Zhen Li, Rong Yu, Quan Shen, Qikai Wang, Jihuan Hou, Qisi Zhang, Xu Si, Ning Wang, Demin Zhou, Wen‐xia Tian, Longlong Si   +14 more
wiley   +1 more source

Cloning of the canine RNA polymerase I promoter and establishment of reverse genetics for influenza A and B in MDCK cells

Virology Journal, 2007
Background Recent incidents where highly pathogenic influenza A H5N1 viruses have spread from avian species into humans have prompted the development of cell-based production of influenza vaccines as an alternative to or replacement of current egg-based ...
Duke Gregory M, Wang Zhaoti
doaj   +1 more source

The SNARE machinery is involved in apical plasma membrane trafficking in MDCK cells. [PDF]

, 1998
We have investigated the controversial involvement of components of the SNARE (soluble N-ethyl maleimide-sensitive factor [NSF] attachment protein [SNAP] receptor) machinery in membrane traffic to the apical plasma membrane of polarized epithelial (MDCK)
Chapin, SJ, Kömüves, LG, Low, SH, Mostov, KE, Weimbs, T, Whiteheart, SW, Wimmer, C   +6 more
core  

TAURINE BEHAVES AS AN OSMOLYTE IN MADIN-DARBY CANINE KIDNEY-CELLS - PROTECTION BY POLARIZED, REGULATED TRANSPORT OF TAURINE [PDF]

, 2014
Using a clonal growth assay, we demonstrated that taurine, a nonperturbing osmolyte accumulated in kidney medulla, brain, and some other tissues of hypertonic experimental animals can function as a nonperturbing osmolyte in Madin-Darby canine kidney ...
UCHIDA, S, NAKANISHI, T, Kwon, H. Moo, PRESTON, AS, HANDLER, JS   +4 more
core   +1 more source

Calcium Shock Enables Efficient and Programmable Particle Delivery for Genome Editing Applications

Advanced Science, EarlyView.
Classical transfection and transduction are inefficient, particularly with confluent cells and organoids, and lack cell type‐specific programmability. This study presents calcium shock (CaSh), a method that dramatically improves particle delivery into single cells, colonies, and organoids.
Nicole Vo, Lorena de Oñate, Maximillian Frank, Xian Hu, Sophie M. Blackburn, Dana V. Foss, Jasmine Villegas, Kunica Asija, Dirk Hockemeyer, Hongxia Fu, Raymond J. Monnat, Ross C. Wilson, Benjamin S. Freedman   +12 more
wiley   +1 more source