Results 41 to 50 of about 16,852 (174)

Axin-1 Regulates Meiotic Spindle Organization in Mouse Oocytes. [PDF]

PLoS ONE, 2016
Axin-1, a negative regulator of Wnt signaling, is a versatile scaffold protein involved in centrosome separation and spindle assembly in mitosis, but its function in mammalian oogenesis remains unknown.
Xiao-Qin He, Yue-Qiang Song, Rui Liu, Yu Liu, Fei Zhang, Zhen Zhang, Yu-Ting Shen, Lin Xu, Ming-Huang Chen, Ya-Long Wang, Bai-Hui Xu, Xiang-Jun Yang, Hai-Long Wang   +12 more
doaj   +1 more source

Revised and Improved Procedure for Immunolocalization of Male Meiotic Chromosomal Proteins and Spindle in Plants without the Use of Enzymes

Plants, 2018
Immunolocalization studies to visualize the distribution of proteins on meiotic chromosomes have become an integral part of studies on meiosis in the model organism Arabidopsis thaliana.
Kuntal De, Li Yuan, Christopher A. Makaroff   +2 more
doaj   +1 more source

Simulated microgravity compromises mouse oocyte maturation by disrupting meiotic spindle organization and inducing cytoplasmic blebbing. [PDF]

PLoS ONE, 2011
In the present study, we discovered that mouse oocyte maturation was inhibited by simulated microgravity via disturbing spindle organization. We cultured mouse oocytes under microgravity condition simulated by NASA's rotary cell culture system, examined ...
Changli Wu, Xinzheng Guo, Fang Wang, Xiaoshuang Li, X Cindy Tian, Li Li, Zhenfang Wu, Shouquan Zhang   +7 more
doaj   +1 more source

Melatonin Protects Against Mdivi-1-Induced Abnormal Spindle Assembly and Mitochondrial Superoxide Production During Porcine Oocyte Maturation

Frontiers in Cell and Developmental Biology, 2021
Mitochondrial division inhibitor 1 (Mdivi-1) reportedly provides a close connection between oocyte maturation and mitochondrial function in pigs. N-acetyl-5-methoxy-tryptamine (melatonin) is known to be a representative antioxidant with the ability to ...
Seul-Gi Yang, Seul-Gi Yang, Seung-Yeon Joe, Seung-Yeon Joe, Jin-Wook Bae, Jin-Wook Bae, Gyeong-Deok Heo, Gyeong-Deok Heo, Hyo-Jin Park, Hyo-Jin Park, Deog-Bon Koo, Deog-Bon Koo   +11 more
doaj   +1 more source

Oocyte Meiotic Spindle Assembly and Function

, 2016
Gametogenesis in animal oocytes reduces the diploid genome content of germline precursors to a haploid state in gametes by discarding ¾ of the duplicated chromosomes through a sequence of two meiotic cell divisions called meiosis I and II. The assembly of the microtubule-based spindle structure that mediates this reduction in genome content remains ...
Aaron F, Severson, George, von Dassow, Bruce, Bowerman   +2 more
openaire   +3 more sources

Meiotic spindle visualization in living human oocytes

Reproductive BioMedicine Online, 2005
A computer-assisted polarization microscopy system (polscope) has made it possible to analyse the meiotic spindle of oocytes subjected to intracytoplasmic sperm injection (ICSI) without affecting their viability. It has been shown that the presence of a detectable birefringent meiotic spindle inside the oocyte cytoplasm of human metaphase II (MII ...
Rienzi L, Ubaldi F, Iacobelli M, Minasi MG, Romano S, Greco E.   +5 more
openaire   +3 more sources

MIS12 Is Required for Kinetochore‐Microtubule Attachment in Oocyte Meiosis

Advanced Science, EarlyView.
A model depicting the role of MIS12 in K‐MT attachment during oocyte meiosis. The presence of MIS12 stabilizes bipolar K‐MT attachments by maintaining the function of NDC80 and its interaction with TUBB. This, in turn, promotes KNL1 assembly and subsequent SAC protein recruitment to kinetochores.
Jian Li, Chun‐Hui Zhang, Yong Wang, Cheng‐Yuan Li, Da‐Rong Wen, Xi Xia, Liang Zhou, Wei‐Ping Qian, Qing‐Yuan Sun, Chang‐Zhong Li   +9 more
wiley   +1 more source

SDF‐1 Attenuates Oocyte Quality Decline During Reproductive Aging Through Autophagy‐Enhanced Stress Granule Scavenging

Advanced Science, EarlyView.
SDF‐1 levels decline significantly with maternal aging. Exogenous supplementation restores meiotic spindle morphology, chromosomal alignment, and mitochondrial function while reducing oxidative stress in aged oocytes. Mechanistically, SDF‐1 enhances autophagic activity to clear accumulated stress granules, thereby rescuing fertilization competence and ...
Rui Long, Meng Wang, Ruolin Mao, Hong Chen, Juepu Zhou, Xiangfei Wang, Zheng Yuan, Ri‐Cheng Chian, Nan Xiao, Qingsong Xi, Yimin Shu, Lei Jin, Lixia Zhu   +12 more
wiley   +1 more source

Arabidopsis AUGMIN8 Contains Two Independent Microtubule Association Domains

Cytoskeleton, EarlyView.
ABSTRACT Plant cells create a plasma membrane‐associated network of microtubules that are nucleated by γ‐tubulin ring complexes primarily through microtubule‐dependent microtubule nucleation (MDMN). This dynamic array organizes into specific patterns in response to developmental and environmental cues to influence primary cell wall construction.
Naveen K. Chana, Timothy Cioffi, Sidney L. Shaw   +2 more
wiley   +1 more source

Spindle Positioning, Meiotic Nonreduction, and Polyploidy in Plants

PLoS Genetics, 2008
Polyploidy, the state of having more than two sets of chromosomes, is common in flowering plants (angiosperms), including the major crops [1]. Indeed, it is estimated that 30%–80% of the angiosperms are polyploids [2], and most diploid plant species, including Arabidopsis thaliana, show evidence of genome duplication in their ancestry [3].
Sebastien Andreuzza, Imran Siddiqi
openaire   +4 more sources