The universal suppressor mutation restores membrane budding defects in the HSV-1 nuclear egress complex by stabilizing the oligomeric lattice. [PDF]
PLoS PathogensNuclear egress is an essential process in herpesvirus replication whereby nascent capsids translocate from the nucleus to the cytoplasm. This initial step of nuclear egress-budding at the inner nuclear membrane-is coordinated by the nuclear egress ...
Elizabeth B Draganova +8 more
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Highly Basic Clusters in the Herpes Simplex Virus 1 Nuclear Egress Complex Drive Membrane Budding by Inducing Lipid Ordering. [PDF]
MBio, 2021 During replication of herpesviruses, capsids escape from the nucleus into the cytoplasm by budding at the inner nuclear membrane. This unusual process is mediated by the viral nuclear egress complex (NEC) that deforms the membrane around the capsid by ...
Gerard C L Wong +2 more
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Recruitment dynamics of ESCRT-III and Vps4 to endosomes and implications for reverse membrane budding [PDF]
eLife, 2017 The ESCRT machinery mediates reverse membrane scission. By quantitative fluorescence lattice light-sheet microscopy, we have shown that ESCRT-III subunits polymerize rapidly on yeast endosomes, together with the recruitment of at least two Vps4 hexamers.
Manuel Alonso Y Adell +15 more
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Value of Models for Membrane Budding [PDF]
Current Opinion in Cell Biology, 2021 The budding of membranes and curvature generation is common to many forms of trafficking in cells. Clathrin-mediated endocytosis, as a prototypical example of trafficking, has been studied in great detail using a variety of experimental systems and methods.
Padmini Rangamani +2 more
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Neuronal Nuclear Membrane Budding Occurs during a Developmental Window Modulated by Torsin Paralogs [PDF]
Cell Reports, 2016 DYT1 dystonia is a neurodevelopmental disease that manifests during a discrete period of childhood. The disease is caused by impaired function of torsinA, a protein linked to nuclear membrane budding.
Lauren M. Tanabe +2 more
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Molecular mechanics underlying flat-to-round membrane budding in live secretory cells [PDF]
Nature Communications, 2022 Shin et al. report the molecular mechanics of membrane budding: actin and dynamin pull membrane inward to form a Λ-shape profile; dynamin helices convert Λ- to Ω-shape by constricting Λ’s base, and then constrict Ω-profile’s pore to form a vesicle.
Wonchul Shin +11 more
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Advanced Materials Interfaces, 2020 Membrane budding is an essential process during various biological activities, such as intercellular communication and transportation of life‐sustaining molecules and signals, which primarily occur with the aid of particular proteins.
Dong June Ahn +2 more
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Programmable DNA shell scaffolds for directional membrane budding [PDF]
Nature CommunicationsIn the pursuit of replicating biological processes at the nanoscale, controlling cellular membrane dynamics has emerged as a key area of interest. Here, we report a system mimicking virus assembly to control directional membrane budding.
Michael T. Pinner, Hendrik Dietz
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Budding and Fission of Membrane Vesicles: A Mini Review
Frontiers in Physics, 2020 In this mini-review, a brief historical survey of the mechanisms which determine the shapes of liposomes and cells and the budding and fission of their membrane is presented.
Samo Penič +2 more
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Structural Basis of Membrane Budding by the Nuclear Egress Complex of Herpesviruses [PDF]
EMBO Journal, 2015 Ekaterina Heldwein
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