Results 121 to 130 of about 29,208 (221)

Lipid Rafts in Signalling, Diseases, and Infections: What Can Be Learned from Fluorescence Techniques?

Membranes
Lipid rafts are dynamic microdomains in the membrane, rich in cholesterol and sphingolipids, that are critical for biological processes like cell signalling, membrane trafficking, and protein organization.
Sara Anselmo, Elisa Bonaccorso, Chiara Gangemi, Giuseppe Sancataldo, Valeria Conti Nibali, Giovanna D’Angelo   +5 more
doaj   +1 more source

Thermosensitive Nanostructured Coacervates Developed by Mixing ABA‐ and AB‐Block Copolymers and Their Unique Sustained Release of Nano‐Micelles in a Regulated Manner

Small, EarlyView.
Polyion complex micelles composed of blends of ABA and AB block copolymers can form coacervates with a hexagonally packed cylinder nanostructure after heating. The resulting coacervates can release the micelles under physiological conditions in a zero‐order kinetics, possessing cargo proteins.
Takumi Egashira, Hiroshi Kamizawa, Yujiro Hamada, Wataru Fushimoto, Hiroshi Y. Yoshikawa, Yuji Higaki, Atsushi Takahara, Takeshi Mori, Yoshiki Katayama, Akihiro Kishimura   +9 more
wiley   +1 more source

Multifunctional Superwetting Sea‐Urchin‐Mimetic Nanosheet‐Based Interface for Remote Oil‐Water Separation

Small, EarlyView.
A fluorine‐free, FBC–rGO coating‐based robust superhydrophobic/superoleophilic sorbent (WCA >154°), enabling rapid, selective oil uptake and efficient oil/water separation. Integrated into a dolphin‐inspired Wi‐Fi mini‐bot with onboard pumping and storage, the platform enables remote, contactless, on‐site oil recovery from hazardous spill zones ...
Surya Kanta Ghadei, Madhu Bhaskaran, Sharath Sriram, Ramasamy Sakthivel, Md. Ataur Rahman   +4 more
wiley   +1 more source

Mechanism elucidation of cell‐selective fluorescent probes

Smart Molecules, EarlyView.
This perspective outlines five mechanistic strategies—Protein‐oriented, carbohydrate‐oriented, lipid‐oriented, gating‐oriented, and metabolism‐oriented live‐cell distinctions—for achieving cell‐selective fluorescence in live‐cell imaging. By classifying probes based on their interactions with proteins, carbohydrates, lipids, transporters, or metabolic ...
Sourav Sarkar, Young‐Tae Chang
wiley   +1 more source

Compartmentalisation in cAMP signalling: A phase separation perspective

British Journal of Pharmacology, EarlyView.
Cells rely on precise spatiotemporal control of signalling pathways to ensure functional specificity. The compartmentalisation of cyclic AMP (cAMP) and protein kinase A (PKA) signalling enables distinct cellular responses within a crowded cytoplasmic space.
Milda Folkmanaite, Manuela Zaccolo
wiley   +1 more source

Distinct calcium regulation of TRPM7 mechanosensitive channels at plasma membrane microdomains visualized by FRET-based single cell imaging. [PDF]

Sci Rep, 2021
Starostina I, Jang YK, Kim HS, Suh JS, Ahn SH, Choi GH, Suk M, Kim TJ.   +7 more
europepmc   +1 more source

New biosensors and transgenic mice for multiplex cGMP imaging

British Journal of Pharmacology, EarlyView.
Background and Purpose Cyclic guanosine monophosphate (cGMP) is a versatile second messenger that is important for human (patho‐)physiology and pharmacotherapy. Live‐cell imaging of cGMP with biosensors allows to elucidate its spatiotemporal dynamics in real time under close‐to‐native conditions. However, to monitor two separate cGMP pools or cGMP/cAMP
Markus Wolters, Martin Thunemann, Barbara Birk, Susanne Feil, Viacheslav O. Nikolaev, Moritz Lehners, Robert Feil   +6 more
wiley   +1 more source

PDE4D and PDE3B orchestrate distinct cAMP microdomains in 3T3‐L1 adipocytes

British Journal of Pharmacology, EarlyView.
Basal conditions: •Ins/PDE3B lowers cytoplasmic cAMP (cyt‐cAMP) without affecting plasma membrane cAMP (pm‐cAMP). •Insulin decreases lipid droplet cAMP (LD‐cAMP) independent of PDE3B. •FGF1/PDE4D modestly reduces both cyt‐ and pm‐cAMP, while PDE4D alone can modulate LD‐cAMP. ISO stimulation: •Ins/PDE3B has minimal impact on cyt‐cAMP.
Johannes Krier, David Spähn, David Arturo Juarez Lopez, Judith L. Nono, Judith Seigner, Lisa Jacob, Siegfried Ussar, Robert Lukowski, Andreas L. Birkenfeld, Gencer Sancar   +9 more
wiley   +1 more source

Dynamics of the CD9 interactome during bacterial infection of epithelial cells by proximity labelling proteomics

The FEBS Journal, EarlyView.
We describe the CD9 interactome in epithelial cells for the first time. The interactome is diverse and dynamic and changes dependent on infection with specific bacteria. We validate these data by showing functional interaction between CD9 with CD44 or CD147. We therefore demonstrate that CD9 is a universal organiser of bacterial adhesion platforms able
Paige A. Wolverson, Isabel Fernandes Parreira, Ruth H. Thompson, Mark O. Collins, Jonathan G. Shaw, Luke R. Green   +5 more
wiley   +1 more source

Primary Human Renal Proximal Tubular Epithelial Cells (pHRPTEpiCs): Shiga Toxin (Stx) Glycosphingolipid Receptors, Stx Susceptibility, and Interaction with Membrane Microdomains. [PDF]

Toxins (Basel), 2021
Detzner J, Klein AL, Pohlentz G, Krojnewski E, Humpf HU, Mellmann A, Karch H, Müthing J.   +7 more
europepmc   +1 more source