Results 81 to 90 of about 102,530 (235)

Mining statistically-solid k-mers for accurate NGS error correction

BMC Genomics, 2018
Background NGS data contains many machine-induced errors. The most advanced methods for the error correction heavily depend on the selection of solid k-mers. A solid k-mer is a k-mer frequently occurring in NGS reads.
Liang Zhao, Jin Xie, Lin Bai, Wen Chen, Mingju Wang, Zhonglei Zhang, Yiqi Wang, Zhe Zhao, Jinyan Li   +8 more
doaj   +1 more source

Emerging experimental and bioinformatic approaches in RNA interference‐based pest control research

Insect Molecular Biology, EarlyView.
Double‐stranded RNA (dsRNA) visualization, RNA‐induced silencing complex (RISC)‐bound sRNA‐seq and RNA degradomics allow investigating dsRNA uptake and processing, guide small interfering RNA selection and RISC‐mediated target mRNA cleavages. Transcriptomic and proteomic profiles of genome‐wide responses to dsRNA, including off‐target and compensatory ...
Doga Cedden
wiley   +1 more source

Cross-sectional study of MERS-CoV-specific RNA and antibodies in animals that have had contact with MERS patients in Saudi Arabia

Journal of Infection and Public Health, 2018
Background: Middle East respiratory syndrome coronavirus (MERS-CoV) is a newly emerged coronavirus that is associated with a severe respiratory disease in humans in the Middle East. The epidemiological profiles of the MERS-CoV infections suggest zoonotic
Samy Kasem, Ibraheem Qasim, Ali Al-Hufofi, Osman Hashim, Ali Alkarar, Ali Abu-Obeida, Albagir Gaafer, Abdelhamid Elfadil, Ahmed Zaki, Ahmed Al-Romaihi, Nasereldeen Babekr, Nadr El-Harby, Raed Hussien, Ali AL-Sahaf, Ali Al-Doweriej, Faisal Bayoumi, Leo L.M. Poon, Daniel K.W. Chu, Malik Peiris, Ranawaka A.P.M. Perera   +19 more
doaj   +1 more source

Microbiome and Pathobiome Characterization in Farmed Barramundi (Lates calcarifer) During and Post Scale Drop Disease Outbreaks

Journal of Fish Diseases, EarlyView.
ABSTRACT Large‐scale double‐digest RAD sequencing (ddRADseq) datasets generated for genotyping are increasingly available in aquaculture, yet their unmapped reads remain largely unexplored for pathogen surveillance. Here, we evaluated the utility and limitations of repurposing unmapped ddRADseq reads to examine pathogen‐associated and disease ...
Nguyen Thanh Vu, Xueyan Shen, Susan Gibson‐Kueh, Maura Carrai, Celestine Terence, Zhi Weng Josiah Poon, Sarah Priyanka Nelson, Saengchan Senapin, Ha Thanh Dong, Jiun‐Yan Loh, Dean R. Jerry, Jose A. Domingos   +11 more
wiley   +1 more source

Do Science Kardashians Get Citation Premium? Self‐Fulfilling Effects of Social Media on Scientific Impact

Kyklos, EarlyView.
ABSTRACT We analyze whether the visibility of scientists on social media affects the number of academic citations. We use the global COVID‐19 pandemic as a quasinatural experiment that exogenously increased public attention and the demand for expertise.
Christian Lessmann, Ali Sina Önder, Maximilian Rose   +2 more
wiley   +1 more source

Bactrian camels shed large quantities of Middle East respiratory syndrome coronavirus (MERS-CoV) after experimental infection

Emerging Microbes and Infections, 2019
In 2012, Middle East respiratory syndrome coronavirus (MERS-CoV) emerged. To date, more than 2300 cases have been reported, with an approximate case fatality rate of 35%.
Danielle R. Adney, Michael Letko, Izabela K. Ragan, Dana Scott, Neeltje van Doremalen, Richard A. Bowen, Vincent J. Munster   +6 more
doaj   +1 more source

Accessory regions and horizontal gene transfer shape the evolution of clonal Colletotrichum nymphaeae infecting strawberry

New Phytologist, EarlyView.
Genetic diversity of strawberry‐infecting Colletotrichum isolates. Summary Rapid adaptation in fungal plant pathogens is often attributed to sexual recombination, yet many important pathogens are largely clonal. We investigated how genetic and phenotypic diversity arises in the predominantly asexual fungus Colletotrichum nymphaeae, the main cause of ...
Joris A. Alkemade, Alan G. Buddie, Anthony Kermode, Timothy G. Barraclough   +3 more
wiley   +1 more source

Structural Definition of a Unique Neutralization Epitope on the Receptor-Binding Domain of MERS-CoV Spike Glycoprotein

Cell Reports, 2018
Summary: The major mechanism of antibody-mediated neutralization of the Middle East respiratory syndrome coronavirus (MERS-CoV) involves competition with the cellular receptor dipeptidyl peptidase 4 (DPP4) for binding to the receptor-binding domain (RBD)
Senyan Zhang, Panpan Zhou, Pengfei Wang, Yangyang Li, Liwei Jiang, Wenxu Jia, Han Wang, Angela Fan, Dongli Wang, Xuanling Shi, Xianyang Fang, Michal Hammel, Shuying Wang, Xinquan Wang, Linqi Zhang   +14 more
doaj   +1 more source

OsPIP2;1 impacts root hydraulic conductance and is a candidate gene for a drought avoidance QTL on rice chromosome 7

Plant Biology, EarlyView.
In this study, we identified a rice plasma membrane intrinsic aquaporin (OsPIP2;1) as the candidate gene for a chromosome 7 locus driving drought avoidance, supported by established and novel physiological experiments measuring root hydraulic conductance in near isogenic and RNAi knockdown lines.
Z. Abubakar, F. S. Khowaja, T. Dasgupta, D. Mieulet, E. Guiderdoni, G. J. Norton, A. H. Price   +6 more
wiley   +1 more source

SARS‐CoV‐2 targets mitochondria, exacerbating COVID‐19 pneumonia

The Journal of Physiology, EarlyView.
Abstract figure legend Following entry into airway epithelial cells (AECs), SARS‐CoV‐2 releases its single‐stranded RNA into the cytoplasm, where it is translated into viral proteins. Several of these viral proteins localize to mitochondria and interact with key mitochondrial components.
Danchen Wu, Asish Dasgupta, Jaskaran Singh Hora, Kuang‐Hueih Chen, Arinjay Banerjee, Stephen L. Archer   +5 more
wiley   +1 more source