In vitro differentiation of Trypanosoma cruzi epimastigotes into metacyclic trypomastigotes using a biphasic medium [PDF]
STAR Protocols, 2021 Summary: The pathogen Trypanosoma cruzi differentiates from epimastigotes (E) into infective metacyclic trypomastigotes (MTs) to invade the mammalian cell.
Jessica Rodríguez Durán +3 more
doaj +5 more sources
Slight temperature changes cause rapid transcriptomic responses in Trypanosoma cruzi metacyclic trypomastigotes [PDF]
Parasites & Vectors, 2020 Background Severe changes in temperature can affect the behavior and ecology of some infectious agents. Trypanosoma cruzi is a protozoan that causes Chagas disease.
Lissa Cruz-Saavedra +5 more
doaj +6 more sources
Morphological comparison of axenic amastigogenesis of trypomastigotes and metacyclic forms of Trypanosoma cruzi [PDF]
Memorias Do Instituto Oswaldo Cruz, 2003 Amastigogenesis occurs first when metacyclic trypomastigotes from triatomine urine differentiate into amastigotes inside mammalian host cells and a secondary process when tissue-derived trypomastigotes invade new cells and differentiate newly to ...
VÍCTOR T Contreras
exaly +6 more sources
Engineering a single-chain antibody against Trypanosoma cruzi metacyclic trypomastigotes to block cell invasion. [PDF]
PLoS ONE, 2019 Trypanosoma cruzi is a flagellate protozoan pathogen that causes Chagas disease. Currently there is no preventive treatment and the efficiency of the two drugs available is limited to the acute phase.
Lara Maria Kalempa Demeu +10 more
doaj +7 more sources
Genetic Structure and Expression of the Surface Glycoprotein GP82, the Main Adhesin of Trypanosoma cruzi Metacyclic Trypomastigotes [PDF]
The Scientific World Journal, 2013 T. cruzi improves the likelihood of invading or adapting to the host through its capacity to present a large repertoire of surface molecules. The metacyclic stage-specific surface glycoprotein GP82 has been implicated in host cell invasion.
Paulo Roberto Ceridorio Correa +4 more
doaj +7 more sources
Comparison of the infectivity of Trypanosoma cruzi insect-derived metacyclic trypomastigotes after mucosal and cutaneous contaminative challenges [PDF]
Memorias do Instituto Oswaldo Cruz, 2013 Trypanosoma cruzi infects humans when infected triatomine vector excreta contaminate breaks in skin or mucosal surfaces. T. cruzi insect-derived metacyclic trypomastigotes (IMT) invade through gastric mucosa after oral challenges without any visible ...
Christopher Steven Eickhoff +3 more
doaj +5 more sources
Production of amastigotes from metacyclic trypomastigotes of Trypanosoma cruzi [PDF]
Memorias do Instituto Oswaldo Cruz, 2002 Attempts to recreate all the developmental stages of Trypanosoma cruzi in vitro have thus far been met with partial success. It is possible, for instance, to produce trypomastigotes in tissue culture and to obtain metacyclic trypomastigotes in axenic ...
Víctor T Contreras +6 more
doaj +4 more sources
Antigen characterization of vector-borne and cultured metacyclic trypomastigotes of Trypanosoma cruzi [PDF]
Revista do Instituto de Medicina Tropical de São Paulo, 1986 Metacyclic trypomastigotes ol the CL strain of Trypanosoma cruzi obtained from triatomid vectors and from axenic cultures were comparatively analysed as to their antigen make-up and immunogenic characteristics.
N. Yoshida +2 more
doaj +4 more sources
Glycosomal Phosphoenolpyruvate Carboxykinase CRISPR/Cas9-Deletion and Its Role in Trypanosoma cruzi Metacyclogenesis and Infectivity in Mammalian Host. [PDF]
FASEB JEffects of PEPCK deficiency in Trypanosoma cruzi bioenergetics and life stages. ABSTRACT Trypanosoma cruzi, the causative agent of Chagas disease, possesses glycosomes—unique organelles that house key metabolic enzymes, several of which are promising therapeutic targets.
Vieira CSD +8 more
europepmc +2 more sources
Trypanosoma cruzi P21 Is a Pleiotropic Protein That Is Involved in Parasite Host Cell Invasion and Intracellular Parasitism. [PDF]
MicrobiologyopenThis study reveals the pleiotropic role of Trypanosoma cruzi P21 protein in the low‐virulence G strain. P21 knockout significantly impairs parasite invasion, intracellular multiplication, and egress in vitro. In vivo, P21 deficiency leads to reduced cardiac tissue parasitism, suggesting its role in establishing and maintaining silent infections.
Uombe NPI +11 more
europepmc +2 more sources