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Proteomic analysis of metacyclic trypomastigotes undergoing Trypanosoma cruzi metacyclogenesis

Journal of Mass Spectrometry, 2007
AbstractTrypanosoma cruzi, the causative agent of the Chagas disease, has a complex life cycle alternating between replicative and noninfective forms with nonreplicative and infective forms of the parasite. Metacyclogenesis is a process that takes place in the invertebrate host, comprising morphogenetic transformation from a noninfective form to an ...
A, Parodi-Talice   +10 more
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Trypanosoma cruzi: Differentiation to metacyclic trypomastigotes in the presence of ADP-ribosyltransferase inhibitors

Experimental Parasitology, 1987
The participation of ADP-ribosyltransferase in Trypanosoma cruzi differentiation to the metacyclic stage was evaluated by analyzing morphogenesis blockage by specific enzyme inhibitors: benzamide, 3-aminobenzamide, theophylline, and nicotinamide. In vitro assays showed a statistically significant reduction in the number of metacyclic forms only when ...
E L, Isola   +2 more
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In vitro Production of Metacyclic Trypomastigotes of Trypanosoma cruzi

The Journal of Parasitology, 1980
Bloodstream-forms of Trypanosoma cruzi ingested by reduviid bugs multiply and differentiate through a series of developmental stages into infective, metacyclic, trypomastigote forms. The intestinal tracts of these bugs provide a suitable physical and chemical environment for the complex developmental cycle of this stercorarian parasite.
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Trypanosoma cruzi:Participation of Intracellular Ca2+during Metacyclic Trypomastigote–Macrophage Interaction

Biochemical and Biophysical Research Communications, 1996
The interaction between Trypanosoma cruzi, the protozoan causative of Chagas's disease, and its host cell is a complex process in which multiple signals including those of Ca2+ are involved. Macrophage cytosolic Ca2+ levels were studied during the interaction of these cells with metacyclic trypomastigotes of T.
S E, Wilkowsky   +2 more
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Immunization and challenge of mice with insect‐derived metacyclic trypomastigotes of Trypanosoma cruzi

Parasite Immunology, 1990
Summary Infection with Trypanosoma cruzi was established in the reduviid vector, Dipetalogaster maximus, by repeated feedings on mice with high parasitaemias, and metacyclic trypomastigotes (IMT) were collected in insect urine after blood meals. The infectivity of IMT in mice was assessed by placing varying numbers of organisms, ranging from 5 to 5000,
L V, Kirchhoff, D F, Hoft
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A comparison of challenge with Trypanosoma cruzi blood-stream trypomastigotes and metacyclic trypomastigotes from Rhodnius prolixus in mice immunized with killed antigens

Transactions of the Royal Society of Tropical Medicine and Hygiene, 1979
Groups of CD-1 mice were immunized with vaccines prepared from freeze-thawed or ultra-sonicated epimastigotes, blood trypomastigotes, or "plasma antigen", of Trypanosoma cruzi strains Y, M1 and Tulahuen. The mice were challenged by the injection of blood stream trypomastigotes obtained from mice, or of metacyclic trypomastigotes harvested from the ...
N, McHardy, R A, Neal
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Infection of Rodent Myeloma Y3-Ag 1.2.3 Cells by Trypanosoma cruzi Metacyclic Trypomastigotes

The Journal of Parasitology, 1984
trapped in a hilly area close to the town of Sanare (9?45'N, 69?36'W, and 1,250 m altitude). The rodent carcass was brought to the parasitology lab for identification of some "whitish bags" attached to the rabbit pericardium. Two cysts were removed, with diameters of 2.8 cm and 2.2 cm, respectively. Hydatid fluid was extracted using a syringe.
D, Chao, J M, Lotz, D G, Dusanic
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Antigenic Differences between Insect- and Culture-Derived Trypanosoma cruzi Metacyclic Trypomastigote Extracts

The American Journal of Tropical Medicine and Hygiene, 1987
Antigenic differences between extracts prepared from insect- and axenic cultured-derived T. cruzi were demonstrated by a protein A-binding immunoradiometric assay.
S, Giovanni de Simone   +2 more
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Structure of the glycosylphosphatidylinositol-anchor of the trans-sialidase from Trypanosoma cruzi metacyclic trypomastigote forms

Molecular and Biochemical Parasitology, 1998
Both, culture-derived and metacyclic trypomastigotes of Trypanosoma cruzi shed a glycoprotein, the shed acute phase antigen, that is responsible for the trans-sialidase activity. In the present work the structure of the glycosylphosphatidylinositol membrane anchor of the trans-sialidase isolated from metacyclic forms was determined.
R, Agusti   +4 more
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Trypanosoma cruzi: the fate of bloodstream trypomastigote, amastigote, metacyclic trypomastigote and epimastigote forms in the peritoneal macrophages of immune and non-immune mice in vivo.

Acta tropica, 1982
Experiments were done in vivo in order to compare the ability of Trypanosoma cruzi bloodstream trypomastigotes (BTr), amastigotes (Am), metacyclic trypomastigotes (MTr) and epimastigotes (Ep) to proliferate in the peritoneal macrophages of the following groups of A/Sn mice: actively immunized with increasing doses of living BTr (AIM); mice passively ...
E, Burger   +3 more
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