Results 21 to 30 of about 33,548 (283)

Chronic Meningitis Investigated via Metagenomic Next-Generation Sequencing [PDF]

JAMA Neurology, 2018
Identifying infectious causes of subacute or chronic meningitis can be challenging. Enhanced, unbiased diagnostic approaches are needed.To present a case series of patients with diagnostically challenging subacute or chronic meningitis using metagenomic next-generation sequencing (mNGS) of cerebrospinal fluid (CSF) supported by a statistical framework ...
Wilson, Michael R, O’Donovan, Brian D, Gelfand, Jeffrey M, Sample, Hannah A, Chow, Felicia C, Betjemann, John P, Shah, Maulik P, Richie, Megan B, Gorman, Mark P, Hajj-Ali, Rula A, Calabrese, Leonard H, Zorn, Kelsey C, Chow, Eric D, Greenlee, John E, Blum, Jonathan H, Green, Gary, Khan, Lillian M, Banerji, Debarko, Langelier, Charles, Bryson-Cahn, Chloe, Harrington, Whitney, Lingappa, Jairam R, Shanbhag, Niraj M, Green, Ari J, Brew, Bruce J, Soldatos, Ariane, Strnad, Luke, Doernberg, Sarah B, Jay, Cheryl A, Douglas, Vanja, Josephson, S Andrew, DeRisi, Joseph L   +31 more
openaire   +4 more sources

Metagenomic next-generation sequencing to diagnose atypical severe scrub typhus

Journal of Microbiology, Immunology and Infection, 2022
Zuan Zhan, Chun-Fan Li, Jian Liu, Chun-Shui Cao, Liang Huang   +4 more
doaj   +3 more sources

Clinical Metagenomic Next-Generation Sequencing for Pathogen Detection [PDF]

Annual Review of Pathology: Mechanisms of Disease, 2019
Nearly all infectious agents contain DNA or RNA genomes, making sequencing an attractive approach for pathogen detection. The cost of high-throughput or next-generation sequencing has been reduced by several orders of magnitude since its advent in 2004, and it has emerged as an enabling technological platform for the detection and taxonomic ...
Gu, Wei, Miller, Steve, Chiu, Charles Y
openaire   +4 more sources

Can metagenomic next-generation sequencing identify the pathogens responsible for culture-negative prosthetic joint infection?

BMC Infectious Diseases, 2020
Background The aims of this study were to (1) evaluate the efficacy and safety of targeted antibiotics for the treatment of culture-negative prosthetic joint infection based on metagenomic next-generation sequencing results and (2) verify the accuracy ...
Chaoxin Wang, Zida Huang, Wenbo Li, Xinyu Fang, Wenming Zhang   +4 more
doaj   +1 more source

MetAMOS: A modular and open source metagenomic assembly and analysis pipeline [PDF]

, 2013
© 2013 Treangen et al. We describe MetAMOS, an open source and modular metagenomic assembly and analysis pipeline. MetAMOS represents an important step towards fully automated metagenomic analysis, starting with next-generation sequencing reads and ...
Astrovskaya, I, Darling, AE, Koren, S, Liu, B, Ondov, B, Phillippy, AM, Pop, M, Sommer, DD, Treangen, TJ   +8 more
core   +5 more sources

Neurobrucellosis: Unexpected Answer From Metagenomic Next-Generation Sequencing [PDF]

Journal of the Pediatric Infectious Diseases Society, 2017
A diagnosis of brucellosis can be difficult because routine culture and serological methods exhibit variable sensitivity and specificity. We present the use of a metagenomic next- generation sequencing assay to diagnose a case of neurobrucellosis from cerebrospinal fluid, resulting in the institution of appropriate antibiotic treatment and a favorable ...
Mongkolrattanothai, Kanokporn, Naccache, Samia N, Bender, Jeffrey M, Samayoa, Erik, Pham, Elizabeth, Yu, Guixia, Dien Bard, Jennifer, Miller, Steve, Aldrovandi, Grace, Chiu, Charles Y   +9 more
openaire   +4 more sources

Metagenomic Next-generation Sequencing: Current Status, Challenges and Prospects of Clinical Application

Xiehe Yixue Zazhi, 2023
Traditional microbial detection methods have difficulty meeting the needs of pathogen detection in the clinic. In recent years, metagenomic next-generation sequencing (mNGS) technology has emerged and been widely used in the diagnosis of various ...
DIAO Zhenli, LI Jinming
doaj   +1 more source

Metagenomic Next-Generation Sequencing Improves Diagnosis of Osteoarticular Infections From Abscess Specimens: A Multicenter Retrospective Study

Frontiers in Microbiology, 2020
Background: We conducted this retrospective study to reveal the accuracy of metagenomic next-generation sequencing (mNGS) for diagnosing osteoarticular infections from fresh abscess specimens obtained from patients in an HIV-naive population.Methods: We ...
Mingwei Zhao, Kai Tang, Fengsheng Liu, Weidong Zhou, Jun Fan, Guangxuan Yan, Shibing Qin, Yu Pang   +7 more
doaj   +1 more source

Metagenomic next-generation sequencing of samples from pediatric febrile illness in Tororo, Uganda. [PDF]

, 2019
Febrile illness is a major burden in African children, and non-malarial causes of fever are uncertain. In this retrospective exploratory study, we used metagenomic next-generation sequencing (mNGS) to evaluate serum, nasopharyngeal, and stool specimens ...
Byaruhanga, Oswald, de Bourcy, Charles, DeRisi, Joseph L, Dimitrov, Boris, Egger, Rebecca, Hsu, Jennifer, Juan, Yun-Fang, Kalantar, Katrina, Kyohere, Mary, Langelier, Charles, Mpimbaza, Arthur, Nakielny, Sara, Ramesh, Akshaya, Rosenthal, Philip J, Ruel, Theodore, Sheu, Jonathan, Wang, James, Wilson, Michael R   +17 more
core   +3 more sources

Metagenomic-based Surveillance of Pacific Coast tick Dermacentor occidentalis Identifies Two Novel Bunyaviruses and an Emerging Human Ricksettsial Pathogen. [PDF]

, 2017
An increasing number of emerging tick-borne diseases has been reported in the United States since the 1970s. Using metagenomic next generation sequencing, we detected nucleic acid sequences from 2 novel viruses in the family Bunyaviridae and an emerging ...
Bouquet, Jerome, Chiu, Charles Y, Delwart, Eric, Lane, Robert S, Melgar, Michael, Swei, Andrea   +5 more
core   +2 more sources