Results 11 to 20 of about 12,894 (157)

Functional dynamics of water in New Delhi metallo-β-lactamase catalysis. [PDF]

Protein Sci
Abstract Water plays a critical role in the hydrolysis of antibiotics by New Delhi metallo‐β‐lactamases (NDMs). The reaction proceeds through a nucleophilic attack on the β‐lactam ring, followed by cleavage of the C–N bond and formation of an anionic nitrogen intermediate. This intermediate is then protonated by a water molecule diffusing from the bulk
Kandhan P, Xiong C, Palzkill T, Tao P.
europepmc   +2 more sources

Isolation and partial purification of a carbapenem-hydrolysing metallo-β-lactamase fromPseudomonas cepacia [PDF]

FEMS Microbiology Letters, 1994
A metallo-beta-lactamase has been isolated from a clinical strain of Pseudomonas cepacia and partially purified using Cibacron blue F3GA coupled agarose. The resulting preparation showed a single band of beta-lactamase activity (pI 8.45) after analytical isoelectric focusing.
I A, Baxter, P A, Lambert
openaire   +2 more sources

Characterization of the active-site residues asparagine 167 and lysine 161 of the IMP-1 metallo β-lactamase [PDF]

FEMS Microbiology Letters, 2001
The roles of lysine at position 161 and asparagine at position 167 in IMP-1 metallo beta-lactamase were studied by site-directed mutagenesis. These residues are highly conserved in metallo beta-lactamases and are thought to be present in the active-site cavity.
S, Haruta, E T, Yamamoto, Y, Eriguchi, T, Sawai   +3 more
openaire   +2 more sources

A rapid method for detecting and distinguishing metallo-β-lactamase-and serine carbapenemase-producing Enterobacteriales using MALDI-TOF MS

Frontiers in Microbiology, 2023
IntroductionCarbapenemase-producing Enterobacteriales (CPE) are a major health threat worldwide, and therefore the development of rapid detection methods is needed.
Xiaopeng Jing, Yanyan Hu, Tingting Wu, Xing Zhang, Shaofeng Luo, Wei Wang, Xiaochun Min, Ruiling Sun, Ji Zeng   +8 more
doaj   +1 more source

Inhibition of IMP-1 metallo-β-lactamase and sensitization of IMP-1-producing bacteria by thioester derivatives [PDF]

FEMS Microbiology Letters, 1999
IMP-1 metallo-beta-lactamase is a transferable carbapenem-hydrolyzing enzyme found in some clinical isolates of Pseudomonas aeruginosa, Serratia marcescens and Klebsiella pneumoniae. Bacteria that express IMP-1 show significantly reduced sensitivity to carbapenems and other beta-lactam antibiotics.
G G, Hammond, J L, Huber, M L, Greenlee, J B, Laub, K, Young, L L, Silver, J M, Balkovec, K D, Pryor, J K, Wu, B, Leiting, D L, Pompliano, J H, Toney   +11 more
openaire   +2 more sources

Occurrences of Metalo-β-lactamase Producing Pseudomonas aeruginosa among Clinical Samples in Kwara state, Nigeria

Journal of Applied Sciences and Environmental Management, 2022
Pseudomonas aeruginosa is a frequent nosocomial pathogen that causes severe disease in many clinical and community settings. The objective of this paper was to isolate Pseudomonas aeruginosa from clinical samples and to investigate the occurrence of ...
O.C. Adekunle, A. Mustapha, G. Odewale , R.O. Ojedele   +3 more
doaj   +1 more source

Detection of metallo-β-lactamase gene blaIMP of Escherichia coli clinical isolates in Sanglah General Hospital Bali

Indonesia Journal of Biomedical Science, 2019
Background: Escherichia coli belong to the family of Enterobacteriaceae that are responsible as one of the leading cause of nosocomial infections. The emergence of Carbapenem-resistant Enterobacteriaceae presents therapeutic challenges for clinicians on choosing the correct type of antibiotics to prescribe.
Muhammad Ardi Afriansyah, Ni Nyoman Sri Budayanti, Komang Januartha Putra Pinatih   +2 more
openaire   +1 more source

Novel IMP-1 metallo-β-lactamase inhibitors can reverse meropenem resistance inEscherichia coliexpressing IMP-1 [PDF]

FEMS Microbiology Letters, 2005
IMP-1 metallo-beta-lactamase is a zinc metalloenzyme that confers antibiotic resistance to bacteria through the hydrolysis of beta-lactam antibiotics. Pathogens that express the enzyme show reduced susceptibility to carbapenems, such as meropenem and imipenem.
Joseph G, Moloughney, Janice, D Thomas, Jeffrey H, Toney   +2 more
openaire   +2 more sources

Production of metallo-β-lactamase among Pseudomonas aeruginosa strains isolated in the State of Sergipe, Brazil

Revista da Sociedade Brasileira de Medicina Tropical, 2015
INTRODUCTION: Acquired production of metallo-β-lactamases is an important mechanism of resistance in Pseudomonas aeruginosa. The objective of this study was to investigate the production of metallo-β-lactamase and the genetic diversity among ceftazidime ...
Lívia Maria do Amorim Costa, Maria Emília de Castro Kling Fleming, Geraldo Renato de Paula, Lenise Arneiro Teixeira, Pedro Juan José Mondino, Sílvia Susana Bona de Mondino, Cláudia Rezende de Vieira Mendonça-Souza   +6 more
doaj   +1 more source

Detection of bla NDM-1 Encoding Imepenemase among the Imipenem-Resistant Gram-Negative Bacilli Isolated from Various Clinical Samples at a Tertiary Care Hospital of Eastern Nepal: A Descriptive Cross-Sectional Study

International Journal of Microbiology, 2020
Background. Carbapenem resistance among Gram-negative isolates caused by the production of the metallo-β-lactamase (MBL) enzyme is being increasingly reported worldwide. One of the newly emerged metallo-β-lactamases is New Delhi metallo-β-lactamase. Data
S. Gautam, N. R. Bhattarai, K. Rai, A. Poudyal, B. Khanal   +4 more
doaj   +1 more source