Results 31 to 40 of about 23,761 (232)

Computational strategies for the design of new enzymatic functions [PDF]

, 2015
In this contribution, recent developments in the design of biocatalysts are reviewed with particular emphasis in the de novo strategy. Studies based on three different reactions, Kemp elimination, Diels–Alder and Retro-Aldolase, are used to illustrate ...
Bertrán, Juan, Moliner, Vicent, Tuñón, Iñaki, Świderek, Katarzyna   +3 more
core   +2 more sources

Bottom‐Up Coacervate‐Based Artificial Cells: Integrating Cellular Hallmarks into Complex Life‐Like Systems

Angewandte Chemie, EarlyView.
Current interest in artificial cell research underscores its potential to deepen our understanding of life's fundamental processes. This review highlights advances in bottom‐up coacervate‐based artificial cell engineering via combined integration of cellular hallmarks.
Arjan Hazegh Nikroo, Angshuman Das, Madelief A. M. Verwiel, Jan C. M. van Hest   +3 more
wiley   +2 more sources

Two‐Dimensional Infrared Spectroscopy Reveals the Presence of a Bridging CO Ligand in Two Catalytic Intermediates of [FeFe] Hydrogenase

Angewandte Chemie, EarlyView.
Two‐dimensional (2D) infrared (IR) spectroscopy provides vastly more structural and functional insight into chemical and biological systems than linear IR spectroscopy. Here, we apply 2D‐IR spectroscopy to two controversially discussed states of the [FeFe] hydrogenase active site, revealing the presence of a bridging carbonyl ligand, which is difficult
Cornelius C. M. Bernitzky, Mathesh Vaithiyanathan, Manon T. Lachmann, Igor V. Sazanovich, Gregory M. Greetham, Patricia Rodríguez‐Maciá, James A. Birrell, Marius Horch   +7 more
wiley   +2 more sources

Vitamin Bs as Potent Anticancer Agents through MMP-2/9 Regulation

Frontiers in Bioscience-Landmark
In recent years, the role of coenzymes, particularly those from the vitamin B group in modulating the activity of metalloenzymes has garnered significant attention in cancer treatment strategies.
Ha Vy Thi Vo, Namdoo Kim, Hyuck Jin Lee
doaj   +1 more source

Structural insights into the effect of active-site mutation on the catalytic mechanism of carbonic anhydrase

IUCrJ, 2020
Enzymes are catalysts of biological processes. Significant insight into their catalytic mechanisms has been obtained by relating site-directed mutagenesis studies to kinetic activity assays. However, revealing the detailed relationship between structural
Jin Kyun Kim, Cheol Lee, Seon Woo Lim, Jacob T. Andring, Aniruddha Adhikari, Robert McKenna, Chae Un Kim   +6 more
doaj   +1 more source

Complementarity of neutron, XFEL and synchrotron crystallography for defining the structures of metalloenzymes at room temperature

IUCrJ, 2022
Room-temperature macromolecular crystallography allows protein structures to be determined under close-to-physiological conditions, permits dynamic freedom in protein motions and enables time-resolved studies.
Tadeo Moreno-Chicano, Leiah M. Carey, Danny Axford, John H. Beale, R. Bruce Doak, Helen M. E. Duyvesteyn, Ali Ebrahim, Robert W. Henning, Diana C. F. Monteiro, Dean A. Myles, Shigeki Owada, Darren A. Sherrell, Megan L. Straw, Vukica Šrajer, Hiroshi Sugimoto, Kensuke Tono, Takehiko Tosha, Ivo Tews, Martin Trebbin, Richard W. Strange, Kevin L. Weiss, Jonathan A. R. Worrall, Flora Meilleur, Robin L. Owen, Reza A. Ghiladi, Michael A. Hough   +25 more
doaj   +1 more source

Insights into enzymatic halogenation from computational studies [PDF]

, 2014
The halogenases are a group of enzymes that have only come to the fore over the last 10 years thanks to the discovery and characterization of several novel representatives.
Senn, Hans M.
core   +2 more sources

Two distinct ferredoxins are essential for nitrogen fixation by the iron nitrogenase in Rhodobacter capsulatus

mBio
Nitrogenases are the only enzymes able to fix gaseous nitrogen into bioavailable ammonia and hence are essential for sustaining life. Catalysis by nitrogenases requires both a large amount of ATP and electrons donated by strongly reducing ferredoxins or ...
Holly Addison, Timo Glatter, Georg K. A. Hochberg, Johannes G. Rebelein   +3 more
doaj   +1 more source

Comparison of the amine/amino acid activation profiles of the β- and γ-carbonic anhydrases from the pathogenic bacterium Burkholderia pseudomallei

Journal of Enzyme Inhibition and Medicinal Chemistry, 2018
The β-class carbonic anhydrase (CA, EC 4.2.1.1) from the pathogenic bacterium Burkholderia pseudomallei, BpsCAβ, that is responsible for the tropical disease melioidosis was investigated for its activation with natural and non-natural amino acids and ...
Daniela Vullo, Sonia Del Prete, Sameh M. Osman, Fatmah A. S. Alasmary, Zeid AlOthman, William A. Donald, Clemente Capasso, Claudiu T. Supuran   +7 more
doaj   +1 more source

Mineral composition of Aegle marmelos (L.) corrêa ash: Exploration of its ability to mimic the function of different metalloenzymes

Chemistry of Inorganic Materials
The motivation to find functional models of different metalloenzymes comes from their potential to develop better catalysts inspired by nature. Generally, metalloenzymes can activate aerial dioxygen due to the metal ions in it.
Kousik Ghosh, K. Vamsee Krishna, T. Vinodkumar, T. Dinesh, R. Balakumar, S. Chitra   +5 more
doaj   +1 more source