Results 21 to 30 of about 23,761 (232)

Quantitative Preparative Native Continuous Polyacrylamide Gel Electrophoresis (QPNC-PAGE) [PDF]

, 2008
QPNC-PAGE, or quantitative preparative native continuous polyacrylamide gel electrophoresis, is a high-resolution technique applied in biochemistry and bioinorganic chemistry to separate proteins by isoelectric point.
Bernd Kastenholz
core   +2 more sources

Metallochaperones Are Needed for Mycobacterium tuberculosis and Escherichia coli Nicotinamidase-Pyrazinamidase Activity. [PDF]

, 2020
Mycobacterium tuberculosis nicotinamidase-pyrazinamidase (PZAse) is a metalloenzyme that catalyzes conversion of nicotinamide-pyrazinamide to nicotinic acid-pyrazinoic acid. This study investigated whether a metallochaperone is required for optimal PZAse
Antiparra, R, Arteaga, H, Bueno, C, Campos, J, Duran, P, Gilman, RH, Huerta, R, Kirwan, DE, Monsalve, A, Sheen, P, Zimic, M   +10 more
core   +1 more source

Anion Inhibition Profile of the β-Carbonic Anhydrase from the Opportunist Pathogenic Fungus Malassezia restricta Involved in Dandruff and Seborrheic Dermatitis

Metabolites, 2019
Carbonic anhydrases (CAs, EC 4.2.1.1) are ubiquitous metalloenzymes, which catalyze the crucial physiological CO2 hydration/dehydration reaction (CO2 + H2O ⇌ HCO3− + H+) balancing the equilibrium between CO2, H2CO3, HCO3− and CO32−.
Sonia Del Prete, Andrea Angeli, Cynthia Ghobril, Julien Hitce, Cécile Clavaud, Xavier Marat, Claudiu T. Supuran, Clemente Capasso   +7 more
doaj   +1 more source

The essential roles of metal ions in insect homeostasis and physiology [PDF]

, 2017
Metal ions play distinct roles in living organisms, including insects. Some, like sodium and potassium, are central players in osmoregulation and ‘blood and guts’ transport physiology, and have been implicated in cold adaptation.
Dow, Julian A.T.
core   +1 more source

Spectroscopical Investigations on the Redox Chemistry of [FeFe]-Hydrogenases in the Presence of Carbon Monoxide

Molecules, 2018
[FeFe]-hydrogenases efficiently catalyzes hydrogen conversion at a unique [4Fe–4S]-[FeFe] cofactor, the so-called H-cluster. The catalytic reaction occurs at the diiron site, while the [4Fe–4S] cluster functions as a redox shuttle.
Konstantin Laun, Stefan Mebs, Jifu Duan, Florian Wittkamp, Ulf-Peter Apfel, Thomas Happe, Martin Winkler, Michael Haumann, Sven T. Stripp   +8 more
doaj   +1 more source

Effects of a Four-Week High-Dosage Zinc Oxide Supplemented Diet on Commensal Escherichia coli of Weaned Pigs [PDF]

, 2019
Strategies to reduce economic losses associated with post-weaning diarrhea in pig farming include high-level dietary zinc oxide supplementation.
Bethe, Astrid, Eichhorn, Inga, Epping, Lennard, Ghazisaeedi, Fereshteh, Johanns, Vanessa C., Lübke-Becker, Antina, Merle, Roswitha, Pfeifer, Yvonne, Semmler, Torsten, Walther, Birgit, Wieler, Lothar H.   +10 more
core   +2 more sources

Comparison of the Sulfonamide Inhibition Profiles of the β- and γ-Carbonic Anhydrases from the Pathogenic Bacterium Burkholderia pseudomallei

Molecules, 2017
We have cloned, purified, and characterized a β-carbonic anhydrase (CA, EC 4.2.1.1), BpsCAβ, from the pathogenic bacterium Burkholderia pseudomallei, responsible for the tropical disease melioidosis.
Daniela Vullo, Sonia Del Prete, Pietro Di Fonzo, Vincenzo Carginale, W. Alexander Donald, Claudiu T. Supuran, Clemente Capasso   +6 more
doaj   +1 more source

Isolation of Acidic, Basic and Neutral Metalloproteins by QPNC-PAGE [PDF]

, 2010
A standard protocol for isolating metalloproteins in complex biological samples is presented using quantitative preparative native continuous polyacrylamide gel electrophoresis (QPNC ...
Bernd Kastenholz, David E. Garfin
core   +2 more sources

Evaluation of Dilution Susceptibility Testing Methods for Aztreonam in Combination with Avibactam against Enterobacterales

Microbiology Spectrum, 2022
As multidrug and pan-resistance among Enterobacterales continue to increase, there is an urgent need for more therapeutic options to treat these infections.
C. J. Kelley, A. Kennedy-Mendez, O. N. Walser, M. T. Thwaites, F. F. Arhin, C. M. Pillar, D. A. Hufnagel   +6 more
doaj   +1 more source

Inhibitory antibodies designed for matrix metalloproteinase modulation [PDF]

, 2019
The family of matrix metalloproteinases (MMPs) consists of a set of biological targets that are involved in a multitude of severe pathogenic events such as different forms of cancers or arthritis.
Fischer, Thomas, Riedl, Rainer
core   +1 more source