Membrane changes during syntrophic interactions of an archaeal-bacterial coculture [PDF]
BMC MicrobiologySyntrophic interactions between bacteria and archaea are vital for anaerobic processes, relying on close cell-to-cell contact for efficient metabolite and electron transfer.
Kerstin Fiege +4 more
doaj +2 more sources
Simultaneous Visualization of Distinct Posttranslational Modification States of β‐Catenin Using Genetic Code Expansion and Click Chemistry [PDF]
ChemBioChem, Volume 27, Issue 1, January 2026.A method to visualize, within the same cell, three distinct posttranslational forms of β‐catenin: wild‐type, phosphomimetic (T41E), and synthetic T41C‐GlcNAc, using genetic code expansion and fluorescence microscopy is developed. This strategy reveals how modifications at T41 alter β‐catenin localization and stability. A novel strategy based on genetic
Christer Abou Anny +8 more
wiley +2 more sources
Archaeal protein containing domain of unknown function 2193 undergoes oligomeric reconfiguration upon iron–sulfur cluster binding [PDF]
FEBS Letters, Volume 599, Issue 16, Page 2318-2333, August 2025.This work presents the characterization of MvoDUF2193, a Methanococcus voltae (Mvo) protein from the domain of unknown function (DUF) 2193 family. We demonstrate that MvoDUF2193 binds a single [4Fe–4S] cluster per subunit and that cluster occupancy regulates the transition from an apo tetramer to a [4Fe–4S] monomeric form. This structural transition is
Emily M. Dieter +8 more
wiley +2 more sources
Defining the methanogenic SECIS element in vivo by targeted mutagenesis [PDF]
RNA BiologyIn all domains of life, Archaea, Eukarya and Bacteria, the unusual amino acid selenocysteine (Sec) is co-translationally incorporated into proteins by recoding a UGA stop codon to a sense codon.
Nils Peiter +5 more
doaj +2 more sources
Shaken not stirred - effect of different mixing modes during the cultivation of methanogenic pure cultures [PDF]
Current Research in Microbial SciencesNumerous cultivation techniques for aerobic microorganisms have been extensively investigated in the field of microbiology. Optimisation of these techniques is important for scientific and economic reasons.
Andja Mullaymeri +4 more
doaj +2 more sources
Adaptation of a methanogen to Fe0 corrosion via direct contact [PDF]
npj Biofilms and MicrobiomesDue to unique genomic adaptations, Methanococcus maripaludis Mic1c10 is highly corrosive when in direct contact with Fe0. A critical adaptation involves increased glycosylation of an extracellular [NiFe]-hydrogenase, facilitating its anchoring to cell ...
Satoshi Kawaichi +3 more
doaj +2 more sources
MjCyc: Rediscovering the pathway-genome landscape of the first sequenced archaeon, Methanocaldococcus (Methanococcus) jannaschii [PDF]
iScienceSummary: The genome of Methanocaldococcus (Methanococcus) jannaschii DSM 2661 was the first Archaeal genome to be sequenced in 1996. Subsequent sequence-based annotation cycles led to its first metabolic reconstruction in 2005.
I. Baltsavia +11 more
doaj +2 more sources
Alternative sources of molybdenum for Methanococcus maripaludis and their implication for the evolution of molybdoenzymes [PDF]
Communications BiologyMolybdoenzymes are essential in global nitrogen, carbon, and sulfur cycling. To date, the only known bioavailable source of molybdenum (Mo) is molybdate.
Devon Payne +5 more
doaj +2 more sources
Massive Cultivation of Methanococcus maripaludis Enabled by Optimized Ni, Se, and Nitrogen Supply [PDF]
ACS OmegaYing Wang +6 more
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Current Research in Biotechnology, 2023 Biosynthetic gene clusters (BGCs) are genes in proximity that are involved in the production of specialized metabolites (SMs) and provide additional in situ advantages to the producing microbes such as antagonism and quorum sensing.
Aya Galal +7 more
doaj +1 more source