Results 41 to 50 of about 8,720 (209)
Formation and Regeneration of Methanococcus voltae Protoplasts [PDF]
Applied and Environmental Microbiology, 1993 Methanococcus voltae cells were converted into protoplasts by suspension in anaerobic 0.1 M Tris-HCl buffer containing 0.4 M sucrose and 0.05 M NaCl as osmoprotectants. Protoplast formation was monitored microscopically by observing the conversion of the typical irregularly shaped (uneven peripheries) coccoid whole cells to ...
Patel, G. +3 more
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CRISPR-Cas9 Toolkit for Genome Editing in an Autotrophic CO2-Fixing Methanogenic Archaeon
Microbiology Spectrum, 2022 The CRISPR-Cas9 system is a robust genome editing tool that is widely applied in eukaryotes and bacteria. However, use of this technique has only been developed for one species of Archaea, a domain of life ranking in parallel with Eukarya and Bacteria ...
Jie Li +6 more
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International Journal of Microbiology, 2010 The genetics and biochemistry of the N-linked glycosylation system of Archaea have been investigated over the past 5 years using flagellins and S layers as reporter proteins in the model organisms, Methanococcus voltae, Methanococcus maripaludis, and ...
Ken F. Jarrell +2 more
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Towards Engineering an Orthogonal Protein Translation Initiation System
Frontiers in Chemistry, 2021 In the last two decades, methods to incorporate non-canonical amino acids (ncAAs) into specific positions of a protein have advanced significantly; these methods have become general tools for engineering proteins. However, almost all these methods depend
Byeong Sung Lee +5 more
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, 2020 These data are the raw mass spectrometry data files for independent genotypes. The corresponding genotypes to sample name are DF1=S2Δupt, DF2=JJΔupt top band, DF3= JJΔupt bottom band, DF4=JJΔuptΔaglB.
Holten, Matthew P +2 more
core +1 more source
Energies, 2021 In this study, the microbiomes linked with the operational parameters in seven mesophilic full-scale AD plants mainly treating food waste (four plants) and sewage sludge (three plants) were analyzed.
Michal Sposob +3 more
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Pathway of glycogen metabolism in Methanococcus maripaludis
Journal of Bacteriology, 1994 Methanococcus maripaludis, a facultatively autotrophic archaebacterium that grows with H2 or formate as the electron donor, does not assimilate sugars and other complex organic substrates. However, glycogen is biosynthesized intracellularly and commonly reaches values of 0.34% of the cellular dry weight in the early stationary phase.
J P, Yu, J, Ladapo, W B, Whitman
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Methanogenic archaea and sulfate reducing bacteria co-cultured on acetate: teamwork or coexistence?
Frontiers in Microbiology, 2015 Acetate is a major product of fermentation processes and an important substrate for sulfate reducing bacteria and methanogenic archaea. Most studies on acetate catabolism by sulfate reducers and methanogens have used pure cultures.
Derya eOzuolmez +5 more
doaj +1 more source
Flagellar Motility and Structure in the Hyperthermoacidophilic Archaeon Sulfolobus solfataricus [PDF]
, 2007 Flagellation in archaea is widespread and is involved in swimming motility. Here, we demonstrate that the structural flagellin gene from the crenarchaeaon Sulfolobus solfataricus is highly expressed in stationary-phase-grown cells and under unfavorable ...
Szabó, Zalán, +19 more
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RNA Biology, 2023 Ribosomal RNA (rRNA) processing and maturation are fundamentally important for ribosome biogenesis, but the mechanisms in archaea, the third form of life, remains largely elusive.
Lei Qi +6 more
doaj +1 more source