Results 21 to 30 of about 4,218 (198)
Redundant synthesis of cysteinyl-tRNACys in Methanosarcina mazei. [PDF]
J Biol Chem, 2008 A subset of methanogenic archaea synthesize the cysteinyl-tRNA(Cys) (Cys-tRNA(Cys)) needed for protein synthesis using both a canonical cysteinyl-tRNA synthetase (CysRS) as well as a set of two enzymes that operate via a separate indirect pathway. In the indirect route, phosphoseryl-tRNA(Cys) (Sep-tRNA(Cys)) is first synthesized by phosphoseryl-tRNA ...
Hauenstein SI, Perona JJ.
europepmc +4 more sources
Immunochemical differences among Methanosarcina mazei S-6 morphologic forms [PDF]
Journal of Bacteriology, 1992 Methanosarcinae are the only archaeobacteria known to undergo major morphologic changes during growth involving unicellular and multicellular forms, and Methanosarcina mazei S-6 is the only strain for which three distinct forms, packets, single cells, and lamina, have so far been observed.
R, Yao +2 more
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Applied Microbiology, 2023 Methane (CH4) has attracted attention as not only one of the hydrogen carriers in terms of energy density, but also synthetic natural gas. In nature, the decomposition of organic compounds is performed with bacterial ecosystems that can produce CH4 ...
Haruki Sawada +3 more
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Compiling a versatile toolbox for inducible gene expression in Methanosarcina mazei. [PDF]
MicrolifeAbstract Methanosarcina mazei is a model organism, providing a platform to explore methanoarchaeal regulation mechanisms on the transcriptional and translational level. This study investigates and evaluates various molecular tools to allow inducible gene expression in M. mazei.
Hüttermann J, Schmitz R.
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Isolation and characterization of disaggregatase from Methanosarcina mazei LYC [PDF]
Applied and Environmental Microbiology, 1990 At certain stages in its growth cycle, Methanosarcina mazei produces an enzyme (disaggregatase) that causes aggregates to separate into single cells. M. mazei S-6 and LYC both produce this enzymatic activity, although the specificities of activities differ. The disaggregatase of M. mazei S-6 had little effect on strain LYC cells, but the disaggregatase
L Y, Xun, R A, Mah, D R, Boone
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Identification of the gene for disaggregatase from Methanosarcina mazei. [PDF]
Archaea, 2008 The gene sequences encoding disaggregatase (Dag), the enzyme responsible for dispersion of cell aggregates of Methanosarcina mazei to single cells, were determined for three strains of M. mazei (S‐6T, LYC and TMA). The dag genes of the three strains were 3234 bp in length and had almost the same sequences with 97% amino acid sequence identities.
Osumi N +7 more
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Proceedings, 2020 A mesophilic methanogenic culture, designated JL01, was isolated from Holocene permafrost in the Russian Arctic. After long-term extensive cultivation at 15 °C, it turned out to be a tied binary culture of archaeal (JL01) and bacterial (Sphaerochaeta ...
Viktoriia Oshurkova +5 more
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Topoisomerase VI is a chirally-selective, preferential DNA decatenase
eLife, 2022 DNA topoisomerase VI (topo VI) is a type IIB DNA topoisomerase found predominantly in archaea and some bacteria, but also in plants and algae. Since its discovery, topo VI has been proposed to be a DNA decatenase; however, robust evidence and a mechanism
Shannon J McKie +5 more
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Nature Communications, 2023 Glutamine synthetases (GS) play central roles in cellular nitrogen assimilation. Although GS active-site formation requires the oligomerization of just two GS subunits, all GS form large, multi-oligomeric machines.
Maria A. Schumacher +4 more
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Frontiers in Microbiology, 2020 Methanogens are the major contributors of greenhouse gas methane and play significant roles in the degradation and transformation of organic matter. These organisms are particularly abundant in Swan Lake, which is a shallow lagoon located in Rongcheng ...
Shiling Zheng +8 more
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