Results 41 to 50 of about 39,725 (303)

Cluster analysis of Plasmodium RNA-seq time-course data identifies stage-specific co-regulated biological processes and regulatory elements [version 1; referees: 1 approved, 2 approved with reservations]

F1000Research, 2016
In this study, we interpreted RNA-seq time-course data of three developmental stages of Plasmodium species by clustering genes based on similarities in their expression profile without prior knowledge of the gene function.
Efejiro Ashano, Itunuoluwa Isewon, Jelili Oyelade, Ezekiel Adebiyi   +3 more
doaj   +1 more source

The hidden genetic reservoir: structural variants as drivers of marine microbial and viral microdiversity

Environmental Microbiome
Background Intraspecific genetic diversity is fundamental to understanding microbial adaptation, evolution, and contributions to ecosystem stability.
Jose M. Haro-Moreno, Juan J. Roda-Garcia, Carmen Molina-Pardines, Mario López-Pérez   +3 more
doaj   +1 more source

Intra-Host Evolution of SARS-CoV-2 During Persistent Infection of Pediatric COVID-19 Patients

Viruses
The Coronavirus disease 2019 (COVID-19) pandemic had a profound global impact, yet children exhibited distinct clinical and epidemiological patterns compared to adults.
Charlie R. Boyle, Tien Doan, Estefany Rios-Guzman, Jessica Maciuch, Lacy M. Simons, Dulce S. Garcia, David B. Williams, Arghavan Alisoltani, Egon A. Ozer, Ramon Lorenzo-Redondo, Judd F. Hultquist   +10 more
doaj   +1 more source

Architecture of the superintegron in Vibrio cholerae: identification of core and unique genes [v1; ref status: indexed, http://f1000r.es/w6]

F1000Research, 2013
Background: Vibrio cholerae, the etiologic agent of cholera, is indigenous to aquatic environments. The V. cholerae genome consists of two chromosomes; the smallest of these harbors a large gene capture and excision system called the superintegron (SI ...
Michel A Marin, Ana Carolina P Vicente
doaj   +1 more source

The (Glg)ABCs of cyanobacteria: modelling of glycogen synthesis and functional divergence of glycogen synthases in Synechocystis sp. PCC 6803

FEBS Letters, EarlyView.
We reconstituted Synechocystis glycogen synthesis in vitro from purified enzymes and showed that two GlgA isoenzymes produce glycogen with different architectures: GlgA1 yields denser, highly branched glycogen, whereas GlgA2 synthesizes longer, less‐branched chains.
Kenric Lee, Dimitrios Bekiari, Sofia Doello, Karl Forchhammer   +3 more
wiley   +1 more source

Insights on biology and evolution from microbial genome sequencing [PDF]

Genome Research, 2005
No field of research has embraced and applied genomic technology more than the field of microbiology. Comparative analysis of nearly 300 microbial species has demonstrated that the microbial genome is a dynamic entity shaped by multiple forces. Microbial genomics has provided a foundation for a broad range of applications, from understanding basic ...
openaire   +2 more sources

The Ecology and Evolution of the Baker’s Yeast Saccharomyces cerevisiae

, 2022
The baker’s yeast Saccharomyces cerevisiae has become a powerful model in ecology and evolutionary biology. A global effort on field survey and population genetics and genomics of S.
Qi-Ming Wang, Feng-Yan Bai, Da-Yong Han, Shou-Fu Duan   +3 more
core   +1 more source

I-PREFR: Inverse PCR-Based Restriction Enzyme FRee Unidirectional Strategy for Rapid Markerless Chromosomal Gene Deletion and Reconstitution in Bacteria Using Suicide Vectors

Bio-Protocol
The standard protocols for allelic exchange using homologous recombination deploy suicide vectors with negative selection markers. However, the use of multiple restriction enzymes to generate sticky ends in the vector and the insert for cloning is time ...
Rekha Rana, Anushika Sharma, Ashish Dutta, Prabhu Patil   +3 more
doaj   +1 more source

Structural insights into an engineered feruloyl esterase with improved MHET degrading properties

FEBS Letters, EarlyView.
A feruloyl esterase was engineered to mimic key features of MHETase, enhancing the degradation of PET oligomers. Structural and computational analysis reveal how a point mutation stabilizes the active site and reshapes the binding cleft, expading substrate scope.
Panagiota Karampa, Konstantinos Makryniotis, Theofani‐Iosifina Sousani, Evangelos Topakas, Vangelis Daskalakis, Maria Dimarogona   +5 more
wiley   +1 more source

Gut microbiome and aging—A dynamic interplay of microbes, metabolites, and the immune system

FEBS Letters, EarlyView.
Age‐dependent shifts in microbial communities engender shifts in microbial metabolite profiles. These in turn drive shifts in barrier surface permeability of the gut and brain and induce immune activation. When paired with preexisting age‐related chronic inflammation this increases the risk of neuroinflammation and neurodegenerative diseases.
Aaron Mehl, Eran Blacher
wiley   +1 more source