The exocyst at the interface between cytoskeleton and membranes in eukaryotic cells
Delivery and final fusion of the secretory vesicles with the relevant target membrane are hierarchically organized and reciprocally interconnected multi-step processes involving not only specific protein-protein interactions, but also specific protein ...
Lukas eSynek +4 more
doaj +1 more source
HIV-1 Gag Protein Associates with F-actin Present in Microfilaments
Several studies have provided evidence that the cellular cytoskeleton may be involved in the assembly and budding of retroviruses. In fractionation studies of HIV-1-infected CEM cells, the majority of the unprocessed Gag polyprotein cofractionated with the cellular cytoskeleton.
REY, OSVALDO +2 more
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Regulation of Apicomplexan Microfilament Dynamics by a Minimal Set of Actin‐Binding Proteins [PDF]
Efficient and rapid host cell invasion is a prerequisite for an intracellular parasitic life style. Pathogens typically induce receptor‐mediated endocytosis and hijack the force‐transducing system of a host cell to gain access to a replication‐competent niche.
Herwig, Schüler, Kai, Matuschewski
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Extrinsic cues, intrinsic cues and microfilaments regulate asymmetric protein localization in Drosophila neuroblasts [PDF]
The Drosophila central nervous system develops from stem cell like precursors called neuroblasts, which divide unequally to bud off a series of smaller daughter cells called ganglion mother cells. Neuroblasts show cell-cycle-specific asymmetric localization of both RNA and proteins: at late interphase, prospero RNA and Inscuteable, Prospero and Staufen
Broadus, Julie, Doe, Chris Q.
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The Arabidopsis thaliana Class II Formin FH13 Modulates Pollen Tube Growth
Formins are a large, evolutionarily conserved family of actin-nucleating proteins with additional roles in regulating microfilament, microtubule, and membrane dynamics.
Eva Kollárová +2 more
doaj +1 more source
Baculovirus Expression and Functional Analysis of Vpa2 Proteins from Bacillus thuringiensis
The mode of action underlying the insecticidal activity of the Bacillus thuringiensis (Bt) binary pesticidal protein Vpb/Vpa (formerly Vip1/Vip2) is uncertain. In this study, three recombinant baculoviruses were constructed using Bac-to-Bac technology to
Oihane Simón +4 more
doaj +1 more source
Raver1, a dual compartment protein, is a ligand for PTB/hnRNPI and microfilament attachment proteins [PDF]
By screening a yeast two-hybrid library with COOH-terminal fragments of vinculin/metavinculin as the bait, we identified a new protein termed raver1. Raver1 is an 80-kD multidomain protein and widely expressed but to varying amounts in different cell lines. In situ and in vitro, raver1 forms complexes with the microfilament-associated proteins vinculin,
Hüttelmaier, Stefan +5 more
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This review explores the integration of microfluidic technology with organoid systems as an innovative platform for studying menopausea complex multi‐organ condition. By enabling precise simulation of inter‐organ communication and hormone responses, microfluidic organoids offer a physiologically relevant model for investigating menopausal syndrome and ...
Qianyi Zhang +4 more
wiley +1 more source
Anticytoskeletal autoantibody to microfilament anchorage sites recognizes novel focal contact proteins. [PDF]
Actin microfilaments are anchored to the plasma membrane at focal contacts. Using an indirect immunofluorescence method, we detected an autoantibody reactive with focal contacts in PtK2, HEp-2, and BHK-21 cells in serum from two patients with early systemic sclerosis. With double immunofluorescence, using the actin-binding drug phalloidin, we localized
J L, Senécal +3 more
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Ovarian Matrisome Dynamics and αvβ3‐Mediated Regulation in Early Follicular Development
The matrisome undergoes dynamic remodeling during early follicular development. Integrin αvβ3 mediates matrisome signals, regulating primordial follicle activation/atresia and secondary follicle growth via Hippo/mTOR pathways, with conserved roles in human ovaries, offering therapeutic targets for ovarian disorders.
Tong Wu +12 more
wiley +1 more source

