Indirect immunofluorescence microscopy was used to localize microfilament-associated proteins in the brush border of mouse intestinal epithelial cells. As expected, antibodies to actin decorated the microfilaments of the microvilli, giving rise to a very
A. Bretscher, K. Weber
semanticscholar +1 more source
Vitronectin at sites of cell-substrate contact in cultures of rat myotubes [PDF]
Affinity-purified antibodies to the serum glycoprotein, vitronectin, were used to study sites of cell-substrate contact in cultures of rat myotubes and fibroblasts.
Baetscher, Manfred+2 more
core +1 more source
Multisynchrony in active microfilaments [PDF]
Biological microfilaments exhibit a variety of synchronization modes. Recent experiments observed that a pair of isolated eukaryotic flagella, coupled solely via the fluid medium, display synchrony at nontrivial phase-lags in addition to in-phase and anti-phase synchrony.
arxiv +1 more source
The exocyst at the interface between cytoskeleton and membranes in eukaryotic cells
Delivery and final fusion of the secretory vesicles with the relevant target membrane are hierarchically organized and reciprocally interconnected multi-step processes involving not only specific protein-protein interactions, but also specific protein ...
Lukas eSynek+4 more
doaj +1 more source
Synthesis of proto-oncogene proteins and cyclins depends on intact microfilaments
It is well established that microfilament disintegration by cytochalasin D (CD) as well as latrunculin (LAT)-A and LAT-B causes an inhibition of S phase entry of various nontransformed cell lines. Our experiments extended these observations to human embryonal diploid fibroblasts (Wi-38). To investigate the question whether this stop of DNA synthesis is
Martin Iwig+2 more
openaire +3 more sources
HIV-1 Gag Protein Associates with F-actin Present in Microfilaments
Several studies have provided evidence that the cellular cytoskeleton may be involved in the assembly and budding of retroviruses. In fractionation studies of HIV-1-infected CEM cells, the majority of the unprocessed Gag polyprotein cofractionated with the cellular cytoskeleton.
Jude Canon, Osvaldo Rey, Paul Krogstad
openaire +3 more sources
The Arabidopsis thaliana Class II Formin FH13 Modulates Pollen Tube Growth
Formins are a large, evolutionarily conserved family of actin-nucleating proteins with additional roles in regulating microfilament, microtubule, and membrane dynamics.
Eva Kollárová+2 more
doaj +1 more source
Actin Cytoskeleton and Golgi Involvement in Barley stripe mosaic virus Movement and Cell Wall Localization of Triple Gene Block Proteins. [PDF]
Barley stripe mosaic virus (BSMV) induces massive actin filament thickening at the infection front of infected Nicotiana benthamiana leaves. To determine the mechanisms leading to actin remodeling, fluorescent protein fusions of the BSMV triple gene ...
Bae, Hanhong+10 more
core +2 more sources
Dependence of protein-induced lipid bilayer deformations on protein shape [PDF]
Membrane proteins typically deform the surrounding lipid bilayer membrane, which can play an important role in the function, regulation, and organization of membrane proteins. Membrane elasticity theory provides a beautiful description of protein-induced lipid bilayer deformations, in which all physical parameters can be directly determined from ...
arxiv +1 more source
A permeabilized cell model for studying cell division: a comparison of anaphase chromosome movement and cleavage furrow constriction in lysed PtK1 cells. [PDF]
After lysis in a Brij 58-polyethylene glycol medium, PtK1 cells are permeable to small molecules, such as erythrosin B, and to proteins, such as rhodamine-labeled FAB, myosin subfragment-1, and tubulin.
CANDE, W. Zacheus+2 more
core +2 more sources