The accuracy, feasibility and challenges of sequencing short tandem repeats using next-generation sequencing platforms. [PDF]
PLoS ONE, 2014 To date we have little knowledge of how accurate next-generation sequencing (NGS) technologies are in sequencing repetitive sequences beyond known limitations to accurately sequence homopolymers.
Monika Zavodna +3 more
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PLoS ONE, 2020 Siberian wild rye (Elymus sibiricus L.), an allotetraploid species, is a potentially high-quality perennial forage crop native to temperate regions. We used fluorescently conjugated oligonucleotides, representing ten repetitive sequences, including 6 ...
Jihong Xie +4 more
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Variable number of tandem repeat markers in the genome sequence of Mycosphaerella fijiensis, the causal agent of black leaf streak disease of banana (Musa spp) [PDF]
, 2010 . We searched the genome of Mycosphaerella fijiensis for molecular markers that would allow population genetics analysis of this plant pathogen. M. fijiensis, the causal agent of banana leaf streak disease, also known as black Sigatoka, is the most ...
Ferreira, C.F. +8 more
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Development of novel multiplex microsatellite polymerase chain reactions to enable high-throughput population genetic studies of Schistosoma haematobium [PDF]
, 2015 © 2015 Webster et al. Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in ...
A. A. Hamidou +23 more
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Vectorette PCR isolation of microsatellite repeat sequences using anchored dinucleotide repeat primers [PDF]
Nucleic Acids Research, 1996 We have developed a vectorette PCR approach to provide an improved method for isolation of microsatellite repeats. The modified procedure relies on PCR amplification using a vectorette-specific primer in combination with one of a panel of anchored dinucleotide repeat primers.
Nicholas Lench
openalex +4 more sources
Microsatellite Expansion Diseases: Repeat Toxicity Found in Translation [PDF]
Neuron, 2017 Sellier et al. (2017) show that translation of expanded CGG repeats in fragile X-associated tremor/ataxia syndrome is initiated at an upstream ACG near-cognate start codon. The resulting polyglycine-containing protein, but not repeat RNA, is pathogenic by disrupting the nuclear lamina.
Fen-Biao, Gao, Joel D, Richter
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Clinical and mechanism research progress on short tandem repeat ⁃ related neuromuscular diseases
Chinese Journal of Contemporary Neurology and Neurosurgery, 2023 Short tandem repeat (STR) is a component of the human genome, the number of repeats of STR is prone to change during DNA replication, and when it exceeds a certain threshold, it will lead to the occurrence of diseases, most of which involve the nervous ...
WAN Ya‑lan +4 more
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BioTechniques, 2003 We developed a technique to improve the efficiency of producing TAA repeat microsatellite markers linked to interspecific conserved genes. Template DNA was prepared from cultures derived from single bacterial artificial chromosome (BAC) colonies using a ...
Geoffrey C. Waldbieser +2 more
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Development and characterization of tri- and tetra-nucleotide polymorphic microsatellite markers for skipjack tuna (Katsuwonus pelamis) [PDF]
, 2012 Skipjack tuna (katsuwonus pelamis) (SJT) is the largest tuna fishery in all the major oceans around the world, and the largest marine fishery in Sri Lanka. Knowledge of genetic population structure and effective population size of SJT in the Indian Ocean
Chand, Vincent +2 more
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RNA biology of disease-associated microsatellite repeat expansions [PDF]
Acta Neuropathologica Communications, 2017 Microsatellites, or simple tandem repeat sequences, occur naturally in the human genome and have important roles in genome evolution and function. However, the expansion of microsatellites is associated with over two dozen neurological diseases. A common denominator among the majority of these disorders is the expression of expanded tandem repeat ...
Kushal J. Rohilla, Keith T. Gagnon
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