Results 21 to 30 of about 28,799 (216)

Rapid development of gene-tagged microsatellite markers from bacterial artificial chromosome clones using anchored TAA repeat primers

open access: yesBioTechniques, 2003
We developed a technique to improve the efficiency of producing TAA repeat microsatellite markers linked to interspecific conserved genes. Template DNA was prepared from cultures derived from single bacterial artificial chromosome (BAC) colonies using a ...
Geoffrey C. Waldbieser   +2 more
doaj   +1 more source

Microsatellite development from genome skimming and transcriptome sequencing: comparison of strategies and lessons from frog species

open access: yesBMC Genomics, 2018
Background Even though microsatellite loci frequently have been isolated using recently developed next-generation sequencing (NGS) techniques, this task is still difficult because of the subsequent polymorphism screening requires a substantial amount of ...
Yun Xia   +4 more
doaj   +1 more source

Functional and immunological relevance of Anaplasma marginale major surface protein 1a sequence and structural analysis.

open access: yesPLoS ONE, 2013
Bovine anaplasmosis is caused by cattle infection with the tick-borne bacterium, Anaplasma marginale. The major surface protein 1a (MSP1a) has been used as a genetic marker for identifying A.
Alejandro Cabezas-Cruz   +14 more
doaj   +1 more source

Distribution and Comparative Analysis of Genomic Microsatellites in Nine Species of Family Sillaginidae

open access: yesFishes
We conducted a comparative analysis of the identified microsatellite sequences across the genomes of nine sillaginids. We examined the microsatellites with motifs ranging from 1 to 6 bp in length and analyzed their distribution and frequency across ...
Yinquan Qu   +4 more
doaj   +1 more source

APMicroDB: A microsatellite database of Acyrthosiphon pisum

open access: yesGenomics Data, 2017
Pea aphids represent a complex genetic system that could be used for QTL analysis, genetic diversity and population genetics studies. Here, we described the development of first microsatellite repeat database of the pea aphid (APMicroDB), accessible at ...
Ritika Bishnoi, Deepak Singla
doaj   +1 more source

USP29‐regulated noncanonical stabilization of the hypoxia‐inducible factor‐α in aggressive prostate cancer

open access: yesMolecular Oncology, EarlyView.
We identify USP29 as the only DUB mirroring CA9 expression, a marker of hypoxia and HIF pathway activation associated with PCA aggressiveness. USP29 stabilizes HIF‐1α and HIF‐2α via a noncanonical mechanism that is independent of PHD/pVHL activity yet relies on proteasomal regulation, establishing USP29 as a previously unrecognized regulator of hypoxic
Amelie S Schober   +16 more
wiley   +1 more source

Analysis of microsatellite polymorphism in inbred knockout mice. [PDF]

open access: yesPLoS ONE, 2012
Previously, we found that the genotype of 42 out of 198 mouse microsatellite loci, which are distributed among all chromosomes except the Y chromosome, changed from monomorphism to polymorphism (CMP) in a genetically modified inbred mouse strain. In this
Baofen Zuo   +8 more
doaj   +1 more source

Profiling neoadjuvant therapy response in rectal cancer using meta‐analysis of publicly available transcriptomic RNA‐seq datasets

open access: yesMolecular Oncology, EarlyView.
This study integrates publicly available transcriptomic datasets to identify molecular signatures associated with response to neoadjuvant chemoradiotherapy in locally advanced rectal cancer. By analyzing a combination of multiple cohorts with bioinformatics approaches, we reveal biological pathways and immune‐related features that may improve ...
Aleksandra Stanojevic   +10 more
wiley   +1 more source

EST-SSR Marker Development from Cannabis Sequence Data: A Resource for Genetic Diversity and Breeding Research

open access: yesISPEC Journal of Agricultural Sciences
Cannabis (Cannabis sativa L.) is a valuable crop utilized for the production of food, fiber, oils, and psychotropic compounds. DNA molecular markers are essential in cannabis breeding research, enabling the identification of genes associated with ...
Ayşe Gül İNCE, Mehmet KARACA
doaj   +1 more source

Microsatellite Markers for the New Zealand Endemic Tree Fuchsia excorticata (Onagraceae)

open access: yesApplications in Plant Sciences, 2013
Premise of the study: Microsatellite markers were developed from a New Zealand endemic understory tree, Fuchsia excorticata, to investigate factors affecting the mating system.
Megan L. Van Etten   +2 more
doaj   +1 more source

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