Results 91 to 100 of about 1,205,418 (341)

Lysate-based pipeline to characterize microtubule-associated proteins uncovers unique microtubule behaviours

Nature Cell Biology, 2022
A. S. Jijumon, Satish Bodakuntla, Mariya Genova, M. Bangera, Violet I Sackett, Laetitia Besse, Fatlinda Maksut, V. Henriot, M. Magiera, M. Sirajuddin, C. Janke   +10 more
semanticscholar   +1 more source

Eco‐Fabricated Nanowave‐Textured Implants Drive Microtubule‐Assisted Nuclear Mechanotransduction and Chromatin Modification: Biophysical Priming for Osteogenesis and Bone Regeneration

Advanced Functional Materials, EarlyView.
Eco‐friendly nanowave‐textured implants produced via femtosecond laser fabrication enhance bone regeneration by orchestrating a precise mechanotransduction cascade. This nanotopography directs mesenchymal stem cell alignment and cytoskeletal organization, triggering changes in nuclear shape and chromatin acetylation that prime cells for osteogenesis ...
Bosu Jeong, Ji‐Young Yoon, Junyong Ahn, Byunghak Lee, Sung‐Min Park, Jae‐Heon Kim, Buuvee Bayarkhangai, Yu Jin Kim, Sak Lee, Jonathan C. Knowles, Moon‐Young Kim, DongJun Lee, Sang‐Hoon Rhee, Hae‐Hyoung Lee, Hae‐Won Kim, Jung‐Hwan Lee   +15 more
wiley   +1 more source

Modification of microtubule steady-state dynamics by phosphorylation of the microtubule-associated proteins.

Proceedings of the National Academy of Sciences of the United States of America, 1981
Phosphorylation of purified microtubule-associated proteins (MAPs) inhibits the rate and extent of MAP-stimulated microtubule assembly. The extent of microtubule assembly is reduced as a result of a decrease in the fraction of tubulin polymerized ...
L. Jameson, M. Caplow
semanticscholar   +1 more source

Machine Learning Guided Design of Nerve‐On‐A‐Chip Platforms with Promoted Neurite Outgrowth

Advanced Functional Materials, EarlyView.
Compared to labor‐intensive trial‐and‐error experimentation, a machine learning (ML)‐guided workflow, incorporating cell viability assays, data augmentation, ensemble modeling, and model interpretation, is developed to accelerate nerve‐on‐a‐chip optimization and uncover data‐driven design principles.
Tsai‐Chun Chung, Chu‐Chun Liang, Min‐Wei Huang, Tianle Chen, Joshua M. Little, Snehi Shrestha, Yi‐Chen Ethan Li, I‐Chi Lee, Po‐Yen Chen   +8 more
wiley   +1 more source

Probing Early Particle‐Cell Membrane Interactions via Single‐Cell and Single‐Particle Interaction Analysis

Advanced Functional Materials, EarlyView.
The pre‐internalization phase of endocytosis remains poorly characterized at single‐cell levels. Single‐cell pre‐internalization mechanics are investigated using advanced robotic techniques. Cancer cells exhibit biphasic adhesion – rapid initial binding followed by reinforcement – while fibroblasts show gradual engagement.
Houari Bettahar, Christos Tapeinos, Oğulcan Işıtman, Carmine D'Amico, Alexandra Correia, Hélder A. Santos, Quan Zhou   +6 more
wiley   +1 more source

Self-assembly of microtubules in extracts of cultured HeLa cells and the identification of HeLa microtubule-associated proteins.

Proceedings of the National Academy of Sciences of the United States of America, 1979
Microtubule protein from HeLa cell extracts was purified by multiple cycles of polymerization and depolymerization in the absence of glycerol or other exogenous polymerization-stimulatory agents.
J. Bulinski, G. Borisy
semanticscholar   +1 more source

Visualizing the microtubule-associated protein tau in the nucleus

Science China Life Sciences, 2014
Although tau is mainly known as an axonal microtubule-associated protein, many studies indicate that it is not restricted to this subcellular compartment. Assessing tau's subcellular distribution, however, is not trivial as is evident from transgenic mouse studies. When human tau is over-expressed, it can be immunohistochemically localized to axons and
Lu, Jing, Li, Ting, He, RongQiao, Bartlett, Perry F., Gotz, Jürgen   +4 more
openaire   +4 more sources

Engineering Topographical Cues to Enhance Neural Regeneration in Spinal Cord Injury: Overcoming Challenges and Advancing Therapies

Advanced Functional Materials, EarlyView.
Spinal cord injury (SCI) poses significant challenges for regeneration due to a series of secondary injury mechanisms. How to use biomaterial approach to target the failed regeneration after SCI remains a critical challenge. This review systematically evaluates current strategies to optimize biomaterial topographies for neurite outgrowth, axonal ...
Wei Xu, Fenghui Wang, Joshua Stein, Siqiao Wang, Pengfei Jiang, Letao Yang, Liming Cheng, Ki‐Bum Lee   +7 more
wiley   +1 more source

Molecular Mechanisms of Extracellular Vesicle Biogenesis and Their Impact on the Design of Custom EVs

Advanced Healthcare Materials, EarlyView.
Extracellular vesicles (EVs) are pivotal mediators of intercellular communication and disease, yet the fundamental mechanisms controlling their biogenesis and cargo selection remain unclear. This limitation hinders their diagnostic utility and therapeutic development.
Luís Carvalho Ferraz, Paulo Pereira, João Vasco Ferreira   +2 more
wiley   +1 more source

Initial phase of dendrite growth: evidence for the involvement of high molecular weight microtubule-associated proteins (HMWP) before the appearance of tubulin

Journal of Cell Biology, 1982
It has recently been shown that high molecular weight microtubule- associated proteins (HMWP) in the brain are present in dendrites and are absent from axons (Matus et al., 1981, Proc. Natl. Acad. Sci. U. S. A. 78:3010-3014).
R. Bernhardt, A. Matus
semanticscholar   +1 more source