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Mitochondrial membrane potential [PDF]
The mitochondrial membrane potential (ΔΨm) generated by proton pumps (Complexes I, III and IV) is an essential component in the process of energy storage during oxidative phosphorylation. Together with the proton gradient (ΔpH), ΔΨm forms the transmembrane potential of hydrogen ions which is harnessed to make ATP.
Ljubava D, Zorova +11 more
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Background Thrombocytopenia is a feared complication of preeclampsia (PE) that can additionally complicate the disease course and that carries a poor prognosis.
Bjoern F. Kraemer +7 more
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Effect of the Mitochondrial Membrane Permeability Transition Pore Promoter Atractyloside on Mitochondrial Damage and Tenderness of Postmortem Beef [PDF]
The objective of this study was to investigate the effect of atractyloside, a promoter of the mitochondrial membrane permeability transition pore (MPTP), on mitochondrial membrane permeability, cristae structure, cytochrome c release and tenderness of ...
LIU Chunmei, WEI Qichao, HAN Dong, LI Xia, ZHANG Chunhui, HUANG Feng
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S-15176 difumarate salt, a derivative of the anti-ischemic metabolic drug trimetazidine, has been intensively studied for its impact on cellular metabolism in animal models of ischemia-reperfusion injury of the liver, heart, spinal cord, and other organs.
Natalia V. Belosludtseva +5 more
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Using flow cytometry for mitochondrial assays
The understanding of how different cell types adapt their metabolism in the face of challenges has been attracting the attention of researchers for many years. Recently, immunologists also started to focus on how the metabolism of immune cells can impact
Lauar de Brito Monteiro +3 more
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Mitochondrial Membrane Potential Assay [PDF]
Mitochondrial function, a key indicator of cell health, can be assessed by monitoring changes in mitochondrial membrane potential (MMP). Cationic fluorescent dyes are commonly used tools to assess MMP. We used a water-soluble mitochondrial membrane potential indicator (m-MPI) to detect changes in MMP in HepG2 cells.
Srilatha, Sakamuru +2 more
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Transport of proteins into mitochondria [PDF]
Translocational intermediates of precursor proteins of ATPase F1β subunit and cytochrome c1 across mitochondrial membranes were analyzed using two different approaches, transport at low temperature and transport after binding of precursor proteins to ...
Neupert, Walter, Schleyer, Manfred
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Biosynthesis of Mitochondrial Porin and Insertion into the Outer Mitochondrial Membrane of Neuruspora crassa [PDF]
Mitochondrial porin, the major protein of the outer mitochondrial membrane is synthesized by free cytoplasmic polysomes. The apparent molecular weight of the porin synthesized in homologous or heterologous cell-free systems is the same as that of the ...
Cassady W. E. +35 more
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Myocardial Imaging for Mitochondrial Membrane Potential
Rest and stress myocardial perfusion imaging, described nearly 4 decades ago ([1][1]), is now performed in >9 million people annually in the United States ([2][2]). The procedure provides clinically useful data regardless of whether the tracer is a monovalent cation such as potassium, thallium ...
H William, Strauss, Heiko, Schöder
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Wafer-scale mitochondrial membrane potential assays [PDF]
It has been reported that mitochondrial metabolic and biophysical parameters are associated with degenerative diseases and the aging process. To evaluate these biochemical parameters, current technology requires several hundred milligrams of isolated mitochondria for functional assays.
Tae-Sun, Lim +4 more
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