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Assembling the mitochondrial outer membrane

Nature Structural & Molecular Biology, 2004
The general preprotein translocase of the outer mitochondrial membrane (TOM complex) transports virtually all mitochondrial precursor proteins, but cannot assemble outer-membrane precursors into functional complexes. A recently discovered sorting and assembly machinery (SAM complex) is essential for integration and assembly of outer-membrane proteins ...
Pfanner, Nikolaus   +3 more
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Mitochondrial Membrane Potential Assay

2022
Mitochondrial function, a key indicator of cell health, can be assessed through monitoring changes in mitochondrial membrane potential (MMP). Cationic fluorescent dyes are commonly used tools to assess MMP. We used a water-soluble mitochondrial membrane potential indicator (m-MPI) to detect changes in MMP in various types of cells, such as HepG2 ...
Srilatha, Sakamuru   +3 more
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The arrangement of mitochondrial membranes and a new structural feature of the inner mitochondrial membranes

Journal of Ultrastructure Research, 1977
Rat heart muscle mitochondria were analyzed by means of high-resolution electron microscopy of ultrathin sections of tissue prepared according to the techniques of Sjostrand and Barajas [J. Ultrastruct. Res. 25, 121 (1968)] . These techniques aim at reducing the extent of changes in protein conformation as compared to conventional preparatory ...
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Lipid metabolism in mitochondrial membranes

Journal of Inherited Metabolic Disease, 2014
AbstractMitochondrial membranes have a unique lipid composition necessary for proper shape and function of the organelle. Mitochondrial lipid metabolism involves biosynthesis of the phospholipids phosphatidylethanolamine, cardiolipin and phosphatidylglycerol, the latter is a precursor of the late endosomal lipid bis(monoacylglycero)phosphate.
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Electrophysiology of the inner mitochondrial membrane

Journal of Bioenergetics and Biomembranes, 1994
The application of electrophysiological techniques to mitochondrial membranes has allowed the observation and partial characterization of several ion channels, including an ATP-sensitive K(+)-selective one, a high-conductance "megachannel", a 107 pS anionic channel and three others studied at alkaline pH's.
Zoratti M, SZABO', ILDIKO'
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Reaction of malonaldehyde with mitochondrial membranes

Mechanisms of Ageing and Development, 1982
Malonaldehyde formed by lipid oxidation is regarded as a main crosslinker in the formation of natural age pigment. To elucidate the mechanism of pigment formation the reaction of malonaldehyde with biomembranes using fluorescence spectroscopy has been studied. Rat liver mitochondrial ghosts or bovine serum albumin were reacted with malonaldehyde.
W X, Balcavage, T K, Alvager
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Lipid Peroxidation in Mitochondrial Membrane

1980
Publisher Summary The principal elements of cytoplasm structure and biological membranes are the phospholipids containing unsaturated fatty acid chains. The protein constituents, amino acids, nucleotides, carbohydrates, or coenzymes do not show such lability as is typical of unsaturated fatty acids in an oxygen-rich atmosphere.
Y A, Vladimirov   +3 more
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Membrane structure: Binary membranes of mitochondrial cristae

Experimental Cell Research, 1969
Abstract Sections of mitochondrial cristae membranes show a five-layered structure consisting of two outer and one central stained region with unstained layers between. Membranous fragments obtained by detergent fractionation of the cristae show three layers with stained layers on each side of a central unstained layer.
K S, Prezbindowski   +3 more
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Incorporation of β-sitosterol into mitochondrial membrane enhances mitochondrial function by promoting inner mitochondrial membrane fluidity

Journal of Bioenergetics and Biomembranes, 2012
Recent findings suggest that mitochondrial membrane fluidity could influence mitochondrial energy metabolism. β-sitosterol (BS) is a common plant sterol that is prevalent in plant oils, nuts, cereals and plant food products. Its chemical structure is very similar to that of cholesterol.
Chun, Shi, Fengming, Wu, Jie, Xu
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A mitochondrial membrane hypothesis of aging

Journal of Theoretical Biology, 1987
Recently it has been demonstrated that the loss of water during aging from rat liver and heart muscle tissue is solely due to water loss from the mitochondria. A hypothesis is presented here which shows the relation between free radical attack to, and water loss from, the mitochondria and the decreased energy availability in aged cells. According to it,
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