Results 21 to 30 of about 37,287 (222)
Current Opinion in Chemical Biology, 2004 This opinion covers the field of molecular beacons (MBs), in which nucleic acids are molecularly engineered to have unique functions for the investigation of biomolecules. Molecular beacons have been used in a variety of formats, and this review discusses four: first, in vitro RNA and DNA monitoring; second, biosensors and biochips based on MBs; third,
Center for Research at the Bio/nano Interface, Department of Chemistry and McKnight Brain Institute, University of Florida, Gainesville, Florida 32611-7200 USA ( host institution ) +3 more
openaire +3 more sources
MicroRNA Detection Using a Double Molecular Beacon Approach: Distinguishing Between miRNA and Pre-miRNA [PDF]
, 2017 MicroRNAs (miRNAs) are small, noncoding RNAs that post-transcriptionally regulate gene expression and are recognized for their roles both as modulators of disease progression and as biomarkers of disease activity, including neurological diseases, cancer,
Baker, Meredith B. +3 more
core +1 more source
In vitro selection of molecular beacons [PDF]
Nucleic Acids Research, 2003 While molecular beacons are primarily known as biosensors for the detection of nucleic acids, it has proven possible to adapt other nucleic acid binding species (aptamers) to function in a manner similar to molecular beacons, yielding fluorescent signals only in the presence of a cognate ligand.
Manjula, Rajendran, Andrew D, Ellington
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Design of a molecular beacon PNA [PDF]
Nucleic Acids Symposium Series, 2002 We have designed a novel dual-labeled PNA oligomer, having both a fluorescent dye and a quencher, by utilizing key compounds 1 and 2. We showed that the designed dual-labeled PNA oligomer works as a molecular beacon PNA. We also investigated the optimization of a stem-loop structure which can supersensitize the function as a molecular beacon PNA.
Fumihiko, Kitagawa +5 more
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Fluorescent Aptasensor for Highly Specific Detection of ATP Using a Newly Screened Aptamer
Sensors, 2022 Owing to the significant roles of adenosine triphosphate (ATP) in diverse biological processes, ATP level is used to research and evaluate the physiological processes of organisms.
Xin Chen +5 more
doaj +1 more source
Seeing is Believing: Dynamic Evolution of Gene Families [PDF]
, 2015 Department of Integrative Biology, Center for Computational Biology, Institute for Cellular and Molecular Biology, The University of Texas at Austin, Austin, TX 78712Supported by National Science Foundation Grants IOS- 0843712 and IOS-1354942 (to H.A.H.)
Harris, Rayna M., Hofmann, Hans A.
core +1 more source
PNA-induced assembly of fluorescent proteins using DNA as a framework [PDF]
, 2013 Controlled alignment of proteins on molecular frameworks requires the development of facile and orthogonal chemical approaches and molecular scaffolds. In this work, protein−PNA conjugates are brought forward as new chemical components allowing efficient
Brunsweld, L, Gholami, Z, Hanley, Q
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tmRNA - a novel high-copy-number RNA diagnostic target - its application for Staphylococcus aureus detection using real-time NASBA [PDF]
, 2009 A real-time nucleic acid sequence-based amplification assay, targeting tmRNA, was designed for the rapid identification of Staphylococcus aureus. The selectivity of the assay was confirmed against a panel of 76 Staphylococcus strains and species and 22 ...
Anonymous +9 more
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BioTechniques, 2000 Homogeneous assays based on real-time fluorescence monitoring during PCR are relevant alternatives for large-scale genotyping of single-nucleotide polymorphisms (SNPs).
I. Täpp +4 more
doaj +1 more source
Genomic and phenotypic evolution of Escherichia coli in a novel citrate-only resource environment
eLife, 2020 Evolutionary innovations allow populations to colonize new ecological niches. We previously reported that aerobic growth on citrate (Cit+) evolved in an Escherichia coli population during adaptation to a minimal glucose medium containing citrate (DM25 ...
Zachary D Blount +11 more
doaj +1 more source