Results 41 to 50 of about 37,287 (222)

Engineering Molecular Beacons for Intracellular Imaging [PDF]

International Journal of Molecular Imaging, 2012
Molecular beacons (MBs) represent a class of nucleic acid probes with unique DNA hairpin structures that specifically target complementary DNA or RNA. The inherent “OFF” to “ON” signal transduction mechanism of MBs makes them promising molecular probes for real-time imaging of DNA/RNA in living cells. However, conventional MBs have been challenged with
Wu, Cuichen Sam, Peng, Lu, You, Mingxu, Han, Da, Chen, Tao, Williams, Kathryn R., Yang, Chaoyong James, Tan, Weihong   +7 more
openaire   +2 more sources

DNA Origami Model for Simple Image Decoding

Discrete Dynamics in Nature and Society, 2020
DNA origami is the application of self-assembly in nanotechnology. The combination of DNA origami and hybridization chain reaction is one of the important application methods of DNA origami. In this paper, DNA origami is used to design the cipher pattern
Risheng Wang, Zhixiang Yin, Jianzhong Cui, Jing Yang, Zhen Tang, Xinmu Yang, Xianya Geng   +6 more
doaj   +1 more source

Interference at the Single Photon Level Along Satellite-Ground Channels [PDF]

, 2016
Quantum interference arising from superposition of states is a striking evidence of the validity of Quantum Mechanics, confirmed in many experiments and also exploited in applications.
Bianco, Giuseppe, Dequal, Daniele, Luceri, Vincenza, Schiavon, Matteo, Tomasin, Marco, Vallone, Giuseppe, Vedovato, Francesco, Villoresi, Paolo   +7 more
core   +2 more sources

In-cell protease assay systems based on trans-localizing molecular beacon proteins using HCV protease as a model system. [PDF]

PLoS ONE, 2013
This study describes a sensitive in-cell protease detection system that enables direct fluorescence detection of a target protease and its inhibition inside living cells.
Jeong Hee Kim, Min Jun Lee, Inhwan Hwang, Hyun Jin Hwang   +3 more
doaj   +1 more source

A Rapid and Easy-to-Perform Method of Nucleic-Acid Based Dengue Virus Diagnosis Using Fluorescence-Based Molecular Beacons

Biosensors, 2021
Detecting dengue virus (DENV) infection in patients as early as possible makes the disease management convenient. Conventionally, DENV infection is diagnosed by ELISA-based methods, but sensitivity and specificity are major concerns.
Soumi Sukla, Prasenjit Mondal, Subhajit Biswas, Surajit Ghosh   +3 more
doaj   +1 more source

Pathways of cellular internalisation of liposomes delivered siRNA and effects on siRNA engagement with target mRNA and silencing in cancer cells [PDF]

, 2018
Design of an efficient delivery system is a generally recognised bottleneck in translation of siRNA technology into clinic. Despite research efforts, cellular processes that determine efficiency of siRNA silencing achieved by different delivery ...
A Homhuan, A Ruyra, A Schroeder, A Wittrup, AD Miller, AL Kiss, C Zhou, C-L Chen, D Dutta, D Vercauteren, Da Kuhn, E Fröhlich, G Caracciolo, G Sahay, Gaurav Sahay, H Tang, J Gilleron, J Hughes, J Rejman, J Ziello, JE Schnitzer, JJ Lu, KM Kitchens, L Kou, L Li, LD Kumar, LJ Hewlett, M Dominska, MA West, O Harush-Frenkel, OP Perumal, P Lönn, PS Pollard, R Kanasty, R Zidovetzki, RL Kanasty, S Grimmer, S Tyagi, S Vranic, SK Rodal, SW Hui, TG Iversen, US Huth, V Capel, VP Torchilin, X-X Zhang, Y Chen, Y-K Oh, ZM Qian   +48 more
core   +4 more sources

Blinking statistics of a molecular beacon triggered by end-denaturation of DNA

, 2005
We use a master equation approach based on the Poland-Scheraga free energy for DNA denaturation to investigate the (un)zipping dynamics of a denaturation wedge in a stretch of DNA, that is clamped at one end.
Ambjörnsson T, Ambjörnsson T, Ambjörnsson T, Ambjörnsson T, Ambjörnsson T Metzler R, Hanke A, Krichevsky O, Ledermann W, Lehn J-M, Meller A, Ralf Metzler, Risken H, Tobias Ambjörnsson, van Kampen N G   +13 more
core   +1 more source

High DNA melting activity of extremophyte Eutrema salsugineum cold shock domain proteins EsCSDP1 and EsCSDP3

Biochemistry and Biophysics Reports, 2016
Plant cold shock domain proteins (CSDP) participate in maintenance of plant stress tolerance and in regulating their development. In the present paper we show that two out of three extremophyte plant Eutrema salsugineum proteins EsCSDP1-3, namely EsCSDP1
Nikolai Zlobin, Konstantin Evlakov, Yakov Alekseev, Konstantin Blagodatskikh, Aleksei Babakov, Vasiliy Taranov   +5 more
doaj   +1 more source

Amplification-free nucleic acid detection with a fluorescence-based waveguide biosensor

Frontiers in Sensors, 2022
Early detection of pathogens using nucleic acids in clinical samples often requires sensitivity at the single-copy level, which currently necessitates time-consuming and expensive nucleic acid amplification. Here, we describe 1) a redesigned flow cell in
Philip A. Kocheril, Kiersten D. Lenz, Daniel E. Jacobsen, Jessica Z. Kubicek-Sutherland   +3 more
doaj   +1 more source

New and Improved Diagnostics for Detection of Drug-Resistant Pulmonary Tuberculosis. [PDF]

, 2011
PURPOSE OF REVIEW: Tuberculosis (TB) remains a global emergency and continues to kill 1.7 million people globally each year. Drug-resistant TB is now well established throughout the world and most TB patients are not being screened for drug resistance ...
Affolabi, Albert, Banerjee, Blakemore, Boehme, Brossier, Bwanga, Caws, Coronel, Drews, Ellner, Ellner, Farnia, Fears, Garza-González, Gupta, Helb, Hillemann, Kiet, Kim, Lemaire, Lin, Ling, Martin, Martin, Martin, Migliori, Migliori, Minion, Morcillo, Neonakis, Nicol, Pai, Palomino, Piersimoni, Sandgren, Sohn, Van Rie, Visalakshi, Visalakshi, Wallis, Wright   +41 more
core   +1 more source