Results 1 to 10 of about 5,040,834 (210)
Nimble Cloning: A Simple, Versatile, and Efficient System for Standardized Molecular Cloning
Frontiers in Bioengineering and Biotechnology, 2020 Molecular cloning is one of the most fundamental technologies in molecular biology, and has been critical for driving biotechnological advances. In this study, we have developed a novel method for standardized molecular cloning.
P. Yan +5 more
semanticscholar +1 more source
QuickStep-Cloning: a sequence-independent, ligation-free method for rapid construction of recombinant plasmids [PDF]
, 2015 Background Molecular cloning is an essential step in biological engineering. Methods involving megaprimer-based PCR of a whole plasmid are promising alternatives to the traditional restriction-ligation-based molecular cloning.
Jajesniak, P., Wong, T.S.
core +2 more sources
Engineering a minimal cloning vector from a pUC18 plasmid backbone with an extended multiple cloning site [PDF]
, 2018 Minimal plasmids play an essential role in many intermediate steps in molecular biology. They can for example be used to assemble building blocks in synthetic biology or be used as intermediate cloning plasmids that are ideal for PCR-based mutagenesis ...
Beyaert, Rudi +2 more
core +1 more source
Journal of Biological Chemistry, 2019 Molecular cloning is a cornerstone of biomedical, biotechnological, and synthetic biology research. As such, improved cloning methodologies can significantly advance the speed and cost of research projects.
J. Watson, J. García-Nafría
semanticscholar +1 more source
A comparative study of Tam3 and Ac transposition in transgenic tobacco and petunia plants [PDF]
, 1989 Transposition of the Anthirrinum majus Tam3 element and the Zea mays Ac element has been monitored in petunia and tobacco plants. Plant vectors were constructed with the transposable elements cloned into the leader sequence of a marker gene ...
A Day +41 more
core +10 more sources
Local cloning of two product states [PDF]
, 2005 Local quantum operations and classical communication (LOCC) put considerable constraints on many quantum information processing tasks such as cloning and discrimination.
Mingsheng Ying +3 more
core +2 more sources
Molecular characterization of cDNA encoding resistance gene-like sequences in Buchloe dactyloides [PDF]
, 2006 Current knowledge of resistance (R) genes and their use for genetic improvement in buffalograss (Buchloe dactyloides [Nutt.] Engelm.) lag behind most crop plants.
Budak, Hikmet +7 more
core +1 more source
Cloning of a human parvovirus by molecular screening of respiratory tract samples.
Proceedings of the National Academy of Sciences of the United States of America, 2005 The identification of new virus species is a key issue for the study of infectious disease but is technically very difficult. We developed a system for large-scale molecular virus screening of clinical samples based on host DNA depletion, random PCR ...
Tobias Allander +5 more
semanticscholar +1 more source
Journal of Neuroscience, 1993 A full-length clone coding for the rat alpha 7 nicotinic receptor subunit was isolated from an adult brain cDNA library and expressed in Xenopus oocytes. A significant proportion of the current through alpha 7-channels is carried by Ca2+.
Philippe Sbgu&a +4 more
semanticscholar +1 more source
Molecular cloning and characterization of B-cadherin, a novel chick cadherin. [PDF]
, 1991 Calcium-dependent cell-cell adhesion is mediated in large part by a set of homologous integral membrane glycoproteins termed cadherins. In this report, antibodies to conserved domains in previously described cadherins have been used to isolate cDNAs ...
Napolitano, EW +3 more
core +2 more sources