Results 11 to 20 of about 472,091 (296)
Isolation, Molecular Cloning, and Expression of yyxA Serine Protease Gene Extracted from Bacillus licheniformis in Escherichia coli [PDF]
مجله بیوتکنولوژی کشاورزی, 2021 Objective Proteases are among the most important industrial enzymes. Microbial proteases, especially from Bacillus sp., are most widely exploited industrially. The aim of this study was isolation, cloning, sequencing, expression, and bioinformatics study
Zahra Aghaei Jeshvaghani, Ramin Hoseini
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HomeRun Vector Assembly System: a flexible and standardized cloning system for assembly of multi-modular DNA constructs. [PDF]
PLoS ONE, 2014 Advances in molecular and synthetic biology call for efficient assembly of multi-modular DNA constructs. We hereby present a novel modular cloning method that obviates the need for restriction endonucleases and significantly improves the efficiency in ...
Ming V Li +6 more
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BioTechniques, 2023 With advancements in multicomponent molecular biological tools, the need for versatile, rapid and cost-effective cloning that enables successful combinatorial assembly of DNA plasmids of interest is becoming increasingly important. Unfortunately, current
Gregory Foran +4 more
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Journal of Dentistry Indonesia, 2015 DNA cloning is one of the most important techniques In the field of molecular biology, with a critical role in analyzing the structure and function of genes and their adjacent regulatory regions.
Elza Ibrahim Auerkari +2 more
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iScience, 2019 Summary: To facilitate the genetic engineering of diverse cyanobacterial strains, we have modified broad-host-range RSF1010-based plasmids to improve transmissibility, increase copy number, and facilitate cloning.
Bryan Bishé +2 more
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Golden GATEway cloning--a combinatorial approach to generate fusion and recombination constructs. [PDF]
PLoS ONE, 2013 The design and generation of DNA constructs is among the necessary but generally tedious tasks for molecular biologists and, typically, the cloning strategy is restricted by available restriction sites.
Stephan Kirchmaier +2 more
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HIF-α Prolyl Hydroxylase Inhibitors and Their Implications for Biomedicine: A Comprehensive Review
Biomedicines, 2021 Oxygen is essential for the maintenance of the body. Living organisms have evolved systems to secure an oxygen environment to be proper. Hypoxia-inducible factor (HIF) plays an essential role in this process; it is a transcription factor that mediates ...
Kiichi Hirota
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BioTechniques, 2019 Minimal plasmids play an essential role in many intermediate steps in molecular biology. For example, they can be used to assemble building blocks in synthetic biology or be used as intermediate cloning plasmids that are ideal for PCR-based mutagenesis ...
Jens Staal +4 more
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BioTechniques, 2020 During molecular cloning, screening bacterial transformants is a time-consuming and labor-intensive process; however, tractable tools that can be applied to various vectors for visual confirmation of desired colonies are limited.
Tomo Kondo, Shigehiko Yumura
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Optimal cloning of PCR fragments by homologous recombination in Escherichia coli. [PDF]
PLoS ONE, 2015 PCR fragments and linear vectors containing overlapping ends are easily assembled into a propagative plasmid by homologous recombination in Escherichia coli. Although this gap-repair cloning approach is straightforward, its existence is virtually unknown
Ana Paula Jacobus, Jeferson Gross
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