Results 241 to 250 of about 3,528,143 (293)
Single‐molecule DNA flow‐stretch assays for high‐throughput DNA–protein interaction studies
FEBS Open Bio, EarlyView.We describe an optimised single‐molecule DNA flow‐stretch assay that visualises DNA–protein interactions in real time. Linear DNA fragments are tethered to a surface and stretched by buffer flow for fluorescence imaging. Using λ and φX174 DNA, this protocol enhances reproducibility and accessibility, providing a versatile approach for studying diverse ...
Ayush Kumar Ganguli +8 more
wiley +1 more source
Optimizing photoactivation of PA‐mCherry for optical pooled CRISPR screens
FEBS Open Bio, EarlyView.Photoactivatable PA‐mCherry finds widespread use to optically tag individual cells. However, confocal 405 nm UV laser‐scanning (normal scan) is much less efficient than widefield UV illumination, limiting the use of PA‐mCherry on confocal instruments. We remedy this limitation by reporting that rapid and repeated confocal scanning with a low‐intensity,
Sravasti Mukherjee +3 more
wiley +1 more source
RoundMi: A quantitative method to analyze mitochondrial morphology in mitotic cells
FEBS Open Bio, EarlyView.RoundMi is a workflow for rapid analysis of mitochondrial morphology in mitotic cells. By combining adaptive preprocessing with automated segmentation and quantification, it enables accurate measurements from single focal plane images, reducing acquisition time and computational demands while remaining compatible with high‐throughput fixed and live ...
Elmira Parvindokht Bararpour +2 more
wiley +1 more source
Journal of the American Chemical Society, 2021 Pumps and motors are essential components of the world as we know it. From the complex proteins that sustain our cells, to the mechanical marvels that power industries, much we take for granted is only possible because of pumps and motors. Although molecular pumps and motors have supported life for eons, it is only recently that chemists have made ...
Yuanning Feng +6 more
openaire +3 more sources
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Nature, 2003
Life implies movement. Most forms of movement in the living world are powered by tiny protein machines known as molecular motors. Among the best known are motors that use sophisticated intramolecular amplification mechanisms to take nanometre steps along protein tracks in the cytoplasm.
Manfred, Schliwa, Günther, Woehlke
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Life implies movement. Most forms of movement in the living world are powered by tiny protein machines known as molecular motors. Among the best known are motors that use sophisticated intramolecular amplification mechanisms to take nanometre steps along protein tracks in the cytoplasm.
Manfred, Schliwa, Günther, Woehlke
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The Physics of Molecular Motors
Accounts of Chemical Research, 2001Molecular motors convert chemical energy into mechanical force and movement. Operating at energies just above those of the thermal bath, these motors experience large fluctuations, and their physical description must be necessarily stochastic. Here, motor operation is described as a biased diffusion on a potential energy surface defined by the ...
C, Bustamante, D, Keller, G, Oster
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Essays in Biochemistry, 2000
The contractile apparatus of muscle is a highly efficient and adaptable mechanism for producing movement and is exploited throughout the animal kingdom. Molecular biology is yielding important insights into the intricate functioning of muscular contraction, especially in the heart, and in explaining the genesis of inherited myopathies.
F D, Davison, L G, D'Cruz, W J, McKenna
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The contractile apparatus of muscle is a highly efficient and adaptable mechanism for producing movement and is exploited throughout the animal kingdom. Molecular biology is yielding important insights into the intricate functioning of muscular contraction, especially in the heart, and in explaining the genesis of inherited myopathies.
F D, Davison, L G, D'Cruz, W J, McKenna
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Journal of Luminescence, 2001
An approach which has been recently introduced to construct microscopic engines is investigated. The main characteristic of the approach is the possibility to determine dynamically the direction of motion of the engines. The engines, which are moving objects on a substrate, are able to move translationally or rotationally and simultaneously perform ...
Porto, M., Urbakh, M., Klafter, J.
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An approach which has been recently introduced to construct microscopic engines is investigated. The main characteristic of the approach is the possibility to determine dynamically the direction of motion of the engines. The engines, which are moving objects on a substrate, are able to move translationally or rotationally and simultaneously perform ...
Porto, M., Urbakh, M., Klafter, J.
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Physical Review Letters, 1995
We present a simple stochastical model for motor molecules that cooperate in large groups. This model could apply for actin-myosin motors in muscles and for motility assays with a high concentration of motor molecules. We calculate the dependence of the velocity on the applied force as a function of ATP concentration and show the existence of a ...
, Jülicher, , Prost
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We present a simple stochastical model for motor molecules that cooperate in large groups. This model could apply for actin-myosin motors in muscles and for motility assays with a high concentration of motor molecules. We calculate the dependence of the velocity on the applied force as a function of ATP concentration and show the existence of a ...
, Jülicher, , Prost
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Science, 1999
ATP is the energy currency of the cell: hydrolysis of ATP into ADP and inorganic phosphate (P) releases energy. The enzyme responsible for generating this energy currency is the ATP synthase. In a Perspective, [Fillingame][1] discusses two new reports ([ Sambongi et al. ][2] and [ Stock et al.
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ATP is the energy currency of the cell: hydrolysis of ATP into ADP and inorganic phosphate (P) releases energy. The enzyme responsible for generating this energy currency is the ATP synthase. In a Perspective, [Fillingame][1] discusses two new reports ([ Sambongi et al. ][2] and [ Stock et al.
openaire +2 more sources