Results 61 to 70 of about 254,467 (221)

Patching Holes in the Chlamydomonas Genome

G3: Genes, Genomes, Genetics, 2016
The Chlamydomonas genome has been sequenced, assembled, and annotated to produce a rich resource for genetics and molecular biology in this well-studied model organism.
Frej Tulin, Frederick R. Cross
doaj   +1 more source

A methionine‐lined active site governs carbocation stabilization and product specificity in a bacterial terpene synthase

FEBS Letters, EarlyView.
This study reveals a unique active site enriched in methionine residues and demonstrates that these residues play a critical role by stabilizing carbocation intermediates through novel sulfur–cation interactions. Structure‐guided mutagenesis further revealed variants with significantly altered product profiles, enhancing pseudopterosin formation. These
Marion Ringel, Carl P. O. Helmer, Shani Zev, Ronja Driller, Emily Buhr, Markus Reinbold, Renana Schwartz, Gabriel Foley, Mikael Boden, Daniel Garbe, Gerhard Schenk, Dan Thomas Major, Bernhard Loll, Thomas Brück   +13 more
wiley   +1 more source

Linking neurogenesis, oligodendrogenesis, and myelination defects to neurodevelopmental disruption in primary mitochondrial disorders

FEBS Letters, EarlyView.
Mitochondrial remodeling shapes neural and glial lineage progression by matching metabolic supply with demand. Elevated OXPHOS supports differentiation and myelin formation, while myelin compaction lowers mitochondrial dependence, revealing mitochondria as key drivers of developmental energy adaptation.
Sahitya Ranjan Biswas, Porter L. Tomsick, Alicia M. Pickrell, Paul D. Morton   +3 more
wiley   +1 more source

Structure and Ligands Interactions of Citrus Tryptophan Decarboxylase by Molecular Modeling and Docking Simulations

Biomolecules, 2019
In a previous work, we in silico annotated protein sequences of Citrus genus plants as putative tryptophan decarboxylase (pTDC). Here, we investigated the structural properties of Citrus pTDCs by using the TDC sequence of Catharanthus roseus as an ...
Angelo Facchiano, Domenico Pignone, Luigi Servillo, Domenico Castaldo, Luigi De Masi   +4 more
doaj   +1 more source

GDNF‐RET signaling drives pulmonary neuroendocrine cell hyperplasia and allergic airway inflammation

FEBS Letters, EarlyView.
A neuroimmune interaction centered on the PNEC‐ILC2 axis in an asthma model. PNEC‐derived CGRP activates nearby ILC2s, which then release cytokines, including IL‐5 and IL‐13. These cytokines promote eosinophil and macrophage infiltration. Subsequently, GDNF produced by these cells induces PNEC hyperplasia via the GDNF receptors, RET and GFRα1 ...
Tasuku Kawano, Erina Ike, Suguru Okada, Tomoko Takahashi   +3 more
wiley   +1 more source

The neural crest‐associated gene ERRFI1 is involved in melanoma progression and resistance toward targeted therapy

Molecular Oncology, EarlyView.
ERRFI1, a neural crest (NC)‐associated gene, was upregulated in melanoma and negatively correlated with the expression of melanocytic differentiation markers and the susceptibility of melanoma cells toward BRAF inhibitors (BRAFi). Knocking down ERRFI1 significantly increased the sensitivity of melanoma cells to BRAFi.
Nina Wang, Qian Sun, Daniel Novak, Lei Zhu, Juliane Poelchen, Tamara Steinfass, Yiman Wang, Viktor Umansky, Jochen Utikal   +8 more
wiley   +1 more source

Regulatory element map of sheep reproductive tissues: functional annotation of tissue-specific strong active enhancers

Frontiers in Veterinary Science
IntroductionComprehensive functional annotation of the genome is crucial for elucidating the molecular mechanisms underlying complex traits and diseases.
Zhu Meng, Zhu Meng, Mingxing Chu, Hao Yang, Shiwen Zhang, Qiangjun Wang, Jiahong Chen, Chunhuan Ren, Zhangyuan Pan, Zhangyuan Pan, Zijun Zhang   +10 more
doaj   +1 more source

In vitro models of cancer‐associated fibroblast heterogeneity uncover subtype‐specific effects of CRISPR perturbations

Molecular Oncology, EarlyView.
Development of therapies targeting cancer‐associated fibroblasts (CAFs) necessitates preclinical model systems that faithfully represent CAF–tumor biology. We established an in vitro coculture system of patient‐derived pancreatic CAFs and tumor cell lines and demonstrated its recapitulation of primary CAF–tumor biology with single‐cell transcriptomics ...
Elysia Saputra, Shamsudheen Karuthedath Vellarikkal, Lixia Li, Hong Sun, Khoa Nguyen, Amber Montano, Suchitra Natarajan, Federica Piccioni, Alex Michael Tamburino, Xin Yu, Aleksandra Katarzyna Olow   +10 more
wiley   +1 more source

An in silico Approach for Structural and Functional Annotation of Salmonella enterica serovar typhimurium Hypothetical Protein R_27 [PDF]

International Journal Bioautomation, 2016
Typhoid fever is a major cause of illness in most developing countries, including Bangladesh. In quest of new potential drug against Typhoid fever, the current study was designed to elucidate structural and functional details of S.
Arif Khan, Hossain Ahmed, Nasreen Jahan, Syed Raju Ali, Al Amin, Mohammad Neaz Morshed   +5 more
doaj  

Improving auditing and annotation of DNA barcode reference libraries of animal COI sequences for Molecular Ecology applications

, 2020
The uncovering and description of Earth’s biodiversity constitute an ongoing and incomplete chapter of the scientific endeavour. Fortunately, biodiversity studies have been greatly benefiting from molecular tools, such as DNA (meta)barcoding, which provide efficient identification tools for biomonitoring and conservation programmes.
openaire   +1 more source