Results 11 to 20 of about 801,490 (249)

Establishing a novel and sensitive assay for bioactivity determination of anti-CD25 antibodies

Heliyon, 2023
Anti-CD25 antibodies have been approved for renal transplantation and has been used prior to and during transplantation by the Food and Drug Administration (FDA).
Maoqin Duan, Chuanfei Yu, Yalan Yang, Zhihao Fu, Chunyu Liu, Jialiang Du, Meng Li, Sha Guo, XiaoJuan Yu, Gangling Xu, Yuting Mei, Lan Wang   +11 more
doaj   +1 more source

Method validation of a bridging immunoassay in combination with acid-dissociation and bead treatment for detection of anti-drug antibody

Heliyon, 2023
Anti-drug antibody (ADA) positivity is correlated with disease relapse risk when treated with monoclonal antibody (mAb) therapeutics. ADA evaluation can assist with interpreting pharmacokinetic, pharmacological, and toxicology results.
Jialiang Du, Yalan Yang, Lingling Zhu, Shaoyi Wang, Chuanfei Yu, Chunyu Liu, Caifeng Long, Baowen Chen, Gangling Xu, Linglong Zou, Lan Wang   +10 more
doaj   +1 more source

Identification, Efficacy, and Stability Evaluation of Succinimide Modification With a High Abundance in the Framework Region of Golimumab

Frontiers in Chemistry, 2022
Succinimide (Asu) is the intermediate for asparagine deamidation in therapeutic proteins, and it can be readily hydrolyzed to form aspartate and iso-aspartate residues. Moreover, Asu plays an important role in the protein degradation pathways, asparagine
Tao Liu, Tao Liu, Tao Liu, Jin Xu, Jin Xu, Jin Xu, Jin Xu, Qingcheng Guo, Dapeng Zhang, Dapeng Zhang, Dapeng Zhang, Jun Li, Weizhu Qian, Weizhu Qian, Weizhu Qian, Huaizu Guo, Huaizu Guo, Huaizu Guo, Huaizu Guo, Xinli Zhou, Sheng Hou, Sheng Hou, Sheng Hou   +22 more
doaj   +1 more source

2D NMR Analysis as a Sensitive Tool for Evaluating the Higher-Order Structural Integrity of Monoclonal Antibody against COVID-19

Pharmaceutics, 2022
The higher-order structure (HOS) of protein therapeutics has been confirmed as a critical quality parameter. In this study, we compared 2D 1H-13C ALSOFAST-HMQC NMR spectra with immunochemical ELISA-based analysis to evaluate their sensitivity in ...
Francesca Cantini, Emanuele Andreano, Ida Paciello, Veronica Ghini, Francesco Berti, Rino Rappuoli, Lucia Banci   +6 more
doaj   +1 more source

Establishment and Functional Characterization of Murine Monoclonal Antibodies Recognizing Neuritin

Antibodies, 2023
Neuritin represents a neurotrophic factor that is not only important in neuronal development and plasticity but also impacts endothelial angiogenesis, cell migration, tumor growth and the production of antibodies by B cells.
Georgia Papadogianni, Inga Ravens, Ahmed Hassan, Andrew Flatley, Regina Feederle, Günter Bernhardt, Hristo Georgiev   +6 more
doaj   +1 more source

Production and characterization of polyclonal antibody against a synthetic peptide from β-actin protein [PDF]

Iranian Journal of Basic Medical Sciences, 2014
Objective(s):Antibodies against actin, as one of the most widely studied structural and multifunctional housekeeping proteins in eukaryotic cells, are used as internal loading controls in western blot analyses.
Nazila Amini, Mohadeseh Naghi Vishteh, Omid Zarei, Reza Hadavi, Negah Ahmadvand, Hodjattallah Rabbani, Mahmood Jeddi-Tehrani   +6 more
doaj  

Production of two SARS-CoV-2 neutralizing antibodies with different potencies in Nicotiana benthamiana

Frontiers in Plant Science, 2022
Monoclonal antibodies are considered to be highly effective therapeutic tools for the treatment of mild to moderate COVID-19 patients. In the present work, we describe the production of two SARS-CoV-2 human IgG1 monoclonal antibodies recognizing the ...
Rachele Frigerio, Carla Marusic, Maria Elena Villani, Chiara Lico, Cristina Capodicasa, Emanuele Andreano, Ida Paciello, Rino Rappuoli, Rino Rappuoli, Anna Maria Salzano, Andrea Scaloni, Selene Baschieri, Marcello Donini   +12 more
doaj   +1 more source

Save the microbes to save the planet. A call to action of the International Union of the Microbiological Societies (IUMS)

One Health Outlook, 2023
Our planet is populated by at least a trillion species of microorganisms. Every life form is sustained by them and they make the planet habitable. Only a minority of them, about 1400 species, cause infectious diseases that are responsible for human ...
Rino Rappuoli, Paul Young, Eliora Ron, Simone Pecetta, Mariagrazia Pizza   +4 more
doaj   +1 more source

Lentil lectin derived from Lens culinaris exhibit broad antiviral activities against SARS-CoV-2 variants

Emerging Microbes and Infections, 2021
The spike (S) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) mutated continuously and newly emerging variants escape from antibody-mediated neutralization raised great concern.
Wenbo Wang, Qianqian Li, Jiajing Wu, Yu Hu, Gang Wu, Chuanfei Yu, Kangwei Xu, Xumei Liu, Qihui Wang, Weijin Huang, Lan Wang, Youchun Wang   +11 more
doaj   +1 more source

Immune response to SARS-CoV-2 Omicron variant in patients and vaccinees following homologous and heterologous vaccinations

Communications Biology, 2022
Antibodies elicited by a triple homologous or heterologous vaccination regimen or following natural SARS-CoV-2 infection combined with a two-dose vaccine course, result in highest neutralization capacity against the Omicron variant BA.1.
Claudia Maria Trombetta, Giulia Piccini, Giulio Pierleoni, Margherita Leonardi, Francesca Dapporto, Serena Marchi, Emanuele Andreano, Ida Paciello, Linda Benincasa, Piero Lovreglio, Nicola Buonvino, Nicola Decaro, Angela Stufano, Eleonora Lorusso, Emilio Bombardieri, Antonella Ruello, Simonetta Viviani, Rino Rappuoli, Eleonora Molesti, Alessandro Manenti, Emanuele Montomoli   +20 more
doaj   +1 more source