Results 11 to 20 of about 1,226,593 (337)

Establishing a novel and sensitive assay for bioactivity determination of anti-CD25 antibodies

Heliyon, 2023
Anti-CD25 antibodies have been approved for renal transplantation and has been used prior to and during transplantation by the Food and Drug Administration (FDA).
Maoqin Duan, Chuanfei Yu, Yalan Yang, Zhihao Fu, Chunyu Liu, Jialiang Du, Meng Li, Sha Guo, XiaoJuan Yu, Gangling Xu, Yuting Mei, Lan Wang   +11 more
doaj   +1 more source

A high-affinity antibody against the CSP N-terminal domain lacks Plasmodium falciparum inhibitory activity [PDF]

, 2020
Malaria is a global health concern and research efforts are ongoing to develop a superior vaccine to RTS,S/AS01. To guide immunogen design, we seek a comprehensive understanding of the protective humoral response against Plasmodium falciparum ...
Bosch, A., Costa, G., Flores-Garcia, Y., Julien, J., Levashina, E., Murugan, R., Oyen, D., Pholcharee, T., Prieto, K., Scally, S., Thai, E., Valleriani, A., Wardemann, H., Weyrich, A., Wilson, I., Wu, N.   +15 more
core   +2 more sources

Method validation of a bridging immunoassay in combination with acid-dissociation and bead treatment for detection of anti-drug antibody

Heliyon, 2023
Anti-drug antibody (ADA) positivity is correlated with disease relapse risk when treated with monoclonal antibody (mAb) therapeutics. ADA evaluation can assist with interpreting pharmacokinetic, pharmacological, and toxicology results.
Jialiang Du, Yalan Yang, Lingling Zhu, Shaoyi Wang, Chuanfei Yu, Chunyu Liu, Caifeng Long, Baowen Chen, Gangling Xu, Linglong Zou, Lan Wang   +10 more
doaj   +1 more source

Human monoclonal antibodies and monoclonal antibody multispecificity [PDF]

British Journal of Cancer, 1987
The majority of human anti-tumour monoclonal antibodies (Mabs) isolated to date have been disappointing. Firstly, they react or cross react with intracellular cytoskeletal proteins or nuclear antigens and therefore are of limited value as blood borne agents.
A M, Campbell, P, Whitford, R E, Leake
openaire   +2 more sources

Systematic comparison of monoclonal versus polyclonal antibodies for mapping histone modifications by ChIP-seq. [PDF]

, 2016
BackgroundThe robustness of ChIP-seq datasets is highly dependent upon the antibodies used. Currently, polyclonal antibodies are the standard despite several limitations: They are non-renewable, vary in performance between lots and need to be validated ...
Busby, Michele, Cornett, Evan M, Epstein, Charles B, Farjoun, Yossi, Gienger, Elizabeth, Gladden, Adrianne, Goren, Alon, Li, Catherine, Nusbaum, Chad, Rothbart, Scott B, Xue, Catherine, Yofe, Ido   +11 more
core   +1 more source

Species- and organ-specificity of secretory proteins derived from human prostate and seminal vesicles [PDF]

, 1990
Polyclonal antibodies against semenogelin (SG) isolated from human seminal vesicle secretion and acid phosphatase (PAP), β‐microseminoprotein (β‐MSP), and Prostate‐Specific Antigen (PSA) derived from human prostatic fluid, as well as a monoclonal ...
Abrahamsson, P., Aumuller, G., Lilja, H., Scheit, K., Seitz, J., von der Kammer, H.   +5 more
core   +2 more sources

Use of OKT3 with ciclosporin and steroids for reversal of acute kidney and liver allograft rejection [PDF]

, 1987
OKT3 monoclonal antibody therapy was added to preexisting baseline immunosuppressive treatment with ciclosporin and steroids to treat rejection in 52 recipients of cadaveric livers and 10 recipients of cadaveric kidneys.
Demetris, AJ, Fung, JJ, Porter, KA
core   +1 more source

Identification, Efficacy, and Stability Evaluation of Succinimide Modification With a High Abundance in the Framework Region of Golimumab

Frontiers in Chemistry, 2022
Succinimide (Asu) is the intermediate for asparagine deamidation in therapeutic proteins, and it can be readily hydrolyzed to form aspartate and iso-aspartate residues. Moreover, Asu plays an important role in the protein degradation pathways, asparagine
Tao Liu, Tao Liu, Tao Liu, Jin Xu, Jin Xu, Jin Xu, Jin Xu, Qingcheng Guo, Dapeng Zhang, Dapeng Zhang, Dapeng Zhang, Jun Li, Weizhu Qian, Weizhu Qian, Weizhu Qian, Huaizu Guo, Huaizu Guo, Huaizu Guo, Huaizu Guo, Xinli Zhou, Sheng Hou, Sheng Hou, Sheng Hou   +22 more
doaj   +1 more source

Inhibition of neurite outgrowth in differentiating mouse N2a neuroblastoma cells by phenyl saligenin phosphate: Effects on MAP kinase (ERK 1/2) activation, neurofilament heavy chain phosphorylation and neuropathy target esterase activity [PDF]

, 2006
Sub-lethal concentrations of the organophosphate phenyl saligenin phosphate (PSP) inhibited the outgrowth of axon-like processes in differentiating mouse N2a neuroblastoma cells (IC50 2.5 μM).
Abou-Donia, Abou-Donia, Alan J. Hargreaves, Barber, Barber, Brunet, Casida, Chambers, Denizot, Ehrich, El-Fawal, Eto, Flaskos, Flaskos, Forshaw, Fowler, Glynn, Glynn, Gupta, Hatch, Henschler, Ian C. Coutts, James, Jensen, John Flaskos, Johnson, Johnson, Johnson, Jortner, Jortner, Keilbaugh, Laemmli, Lee, Li, Lowry, Magdalini Sachana, Mary Bountouri, Maxine J. Fowler, Nixon, Nomeir, Nomeir, Nostrandt, Nostrandt, Paul Glynn, Perron, Rosenstock, Schmuck, Sharma, Song, Suwita, Towbin, Van Teinhoven, Veeranna, Veronesi, W. Graham McLean, Wayne Harris, Zhao   +56 more
core   +1 more source

2D NMR Analysis as a Sensitive Tool for Evaluating the Higher-Order Structural Integrity of Monoclonal Antibody against COVID-19

Pharmaceutics, 2022
The higher-order structure (HOS) of protein therapeutics has been confirmed as a critical quality parameter. In this study, we compared 2D 1H-13C ALSOFAST-HMQC NMR spectra with immunochemical ELISA-based analysis to evaluate their sensitivity in ...
Francesca Cantini, Emanuele Andreano, Ida Paciello, Veronica Ghini, Francesco Berti, Rino Rappuoli, Lucia Banci   +6 more
doaj   +1 more source