Results 181 to 190 of about 5,253 (224)

Elucidation of the symbiotic incompatibility mechanisms between <i>Vigna radiat</i>a and <i>Bradyrhizobium vignae</i> ORS3257 mediated by nodulation outer protein P2. [PDF]

open access: yesiScience
Songwattana P   +16 more
europepmc   +1 more source

Reduction of Phenoxyl Radicals Mediated by Monodehydroascorbate Reductase

Biochemical and Biophysical Research Communications, 2000
Monodehydroascorbate (MDA) reductase catalyzes the reduction of MDA, the only organic radical substrate for the enzyme reported so far. Here, we show that cucumber MDA reductase is also capable of reducing phenoxyl radicals which are generated by horseradish peroxidase (HRP) with H2O2.
Y, Sakihama   +4 more
openaire   +2 more sources

Purification and characterization of monodehydroascorbate reductase from soybean root nodules

Archives of Biochemistry and Biophysics, 1992
Soybean (Glycine max (L.) Merr.) root nodules contain the enzymes of the ascorbate-glutathione cycle as an important defense against activated forms of oxygen. A key enzyme in this cycle--monodehydroascorbate reductase (MR)--was purified 646-fold and appeared as a single band on SDS-PAGE with silver or Coomassie blue staining. Purified MR contained 0.7
D A, Dalton, L, Langeberg, M, Robbins
openaire   +2 more sources

Purification and cDNA Cloning of Chloroplastic Monodehydroascorbate Reductase from Spinach

Bioscience, Biotechnology, and Biochemistry, 2005
The chloroplastic isoform of monodehydroascorbate (MDA) radical reductase was purified from spinach chloroplasts and leaves. The cDNA of chloroplastic MDA reductase was cloned, and its deduced amino acid sequence, consisting of 497 residues, showed high homology with those of putative organellar MDA reductases deduced from cDNAs of several plants.
Satoshi, Sano   +9 more
openaire   +2 more sources

Ascorbic acid and photosynthesis I. “Monodehydroascorbic acid” reductase of chloroplasts

Biochimica et Biophysica Acta, 1958
An enzyme system catalyzing the oxidation of DPNH and TPNH in the presence of ascorbic acid and of an ascorbic acid-oxidizing agent such as Cu++ ions or ascorbic acid oxidase has been extracted from the acetone powder of whole, washed spinach chloroplasts This system is unable to reduce dehydroascorbic acid, and closely corresponds to the reduced ...
E, MARRE, O, ARRIGONI
openaire   +2 more sources

Characterization and analysis of the promoter region of monodehydroascorbate reductase 4 (CpMDAR4) in papaya

Plant Reproduction, 2022
Differential spatial and temporal expression patterns due to regulatory cis-elements and two different isoforms are detected among CpMDAR4 alleles in papaya. The aim of this research was to study the effects of cis-element differences between the X, Y and Yh alleles on the expression of CpMDAR4, a potential candidate gene for sex differentiation in ...
Dessireé Zerpa-Catanho   +2 more
openaire   +2 more sources

Crystallization and preliminary crystallographic analysis of monodehydroascorbate radical reductase from cucumber

Acta Crystallographica Section D Biological Crystallography, 2004
Monodehydroascorbate (MDA) radical reductase (EC 1.6.5.4) is an FAD enzyme that catalyzes the univalent reduction of MDA radical to ascorbate using NAD(P)H as an electron donor. The recombinant MDA reductase from cucumber was crystallized using polyethylene glycol 6000 as a precipitant.
Satoshi, Sano   +7 more
openaire   +2 more sources

Overexpression of dehydroascorbate reductase, but not monodehydroascorbate reductase, confers tolerance to aluminum stress in transgenic tobacco

Planta, 2009
Aluminum (Al) inhibits plant growth partly by causing oxidative damage that is promoted by reactive oxygen species and can be prevented by improving antioxidant capacity. Ascorbic acid (AsA), the most abundant antioxidant in plants, is regenerated by the action of monodehydroascorbate reductase (MDAR) and dehydroascorbate reductase (DHAR).
Lina, Yin   +7 more
openaire   +2 more sources

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