Results 1 to 10 of about 254,310 (345)

Orchestration an extracellular lipase production from Aspergillus niger MYA 135: biomass morphology and fungal physiology

AMB Express, 2021
The impact of biomass morphology and culture conditions on fungal fermentation was widely reviewed in the literature. In this work, we presented three independent experiments in order to evaluate the influence of some of those input factors on a lipase ...
Hebe Natalia Salvatierra, Erika Lucía Regner, Mario Domingo Baigorí, Licia María Pera   +3 more
doaj   +1 more source

3D in vitro morphogenesis of human intestinal epithelium in a gut-on-a-chip or a hybrid chip with a cell culture insert

Nature Protocols, 2022
Human intestinal morphogenesis establishes 3D epithelial microarchitecture and spatially organized crypt–villus characteristics. This unique structure is necessary to maintain intestinal homeostasis by protecting the stem cell niche in the basal crypt ...
Woojung Shin, Hyun Jung Kim
semanticscholar   +1 more source

Comparative analysis of liver involvement caused by two DENV-2 lineages using an immunocompetent murine model

Scientific Reports, 2021
Dengue (DEN) is the most prevalent arbovirus among humans, and four billion people live at risk of infection. The clinical manifestations of DEN are variable, and the disease may present subclinically or asymptomatically.
Fernanda Cunha Jácome, Gabriela Cardoso Caldas, Arthur da Costa Rasinhas, Ana Luisa Teixeira de Almeida, Daniel Dias Coutinho de Souza, Amanda Carlos Paulino, Raphael Leonardo, Ortrud Monika Barth, Flavia Barreto dos Santos, Débora Ferreira Barreto-Vieira   +9 more
doaj   +1 more source

Targeted mechanical stimulation via magnetic nanoparticles guides in vitro tissue development

Nature Communications, 2023
Tissues take shape through a series of morphogenetic movements guided by local cell-scale mechanical forces. Current in vitro approaches to recapitulate tissue mechanics rely on uncontrolled self-organization or on the imposition of extrinsic and ...
Abdel Rahman Abdel Fattah, Niko Kolaitis, Katrien Van Daele, Brian Daza, Andika Gregorius Rustandi, Adrian Ranga   +5 more
doaj   +1 more source

High-Throughput Imaging of Blood Flow Reveals Developmental Changes in Distribution Patterns of Hemodynamic Quantities in Developing Zebrafish

Frontiers in Physiology, 2022
Mechanical forces from blood flow and pressure (hemodynamic forces) contribute to the formation and shaping of the blood vascular network during embryonic development. Previous studies have demonstrated that hemodynamic forces regulate signaling and gene
Swe Soe Maung Ye, Jung Kyung Kim, Jung Kyung Kim, Nuria Taberner Carretero, Li-Kun Phng   +4 more
doaj   +1 more source

Defective metabolic programming impairs early neuronal morphogenesis in neural cultures and an organoid model of Leigh syndrome

Nature Communications, 2021
Leigh syndrome (LS) is a severe manifestation of mitochondrial disease in children and is currently incurable. The lack of effective models hampers our understanding of the mechanisms underlying the neuronal pathology of LS. Using patient-derived induced
Gizem Inak, A. Rybak-Wolf, P. Lisowski, T. M. Pentimalli, R. Jüttner, Petar Glažar, K. Uppal, E. Bottani, D. Brunetti, Christopher Secker, Annika Zink, D. Meierhofer, Marie-Thérèse Henke, Monishita Dey, Ummi Ciptasari, Barbara Mlody, T. Hahn, Maria Berruezo-Llacuna, Nikos Karaiskos, M. Di Virgilio, J. Mayr, S. Wortmann, J. Priller, M. Gotthardt, Dean P. Jones, E. Mayatepek, W. Stenzel, S. Diecke, R. Kühn, E. Wanker, N. Rajewsky, M. Schuelke, A. Prigione   +32 more
semanticscholar   +1 more source

Morphogenesis and cytopathic effect of SARS-CoV-2 infection in human airway epithelial cells

Nature Communications, 2020
SARS-CoV-2, a β-coronavirus, has rapidly spread across the world, highlighting its high transmissibility, but the underlying morphogenesis and pathogenesis remain poorly understood.
N. Zhu, Wenling Wang, Zhidong Liu, C. Liang, Wen Wang, F. Ye, Baoying Huang, Li Zhao, Huijuan Wang, Wei-min Zhou, Yao Deng, Longfei Mao, C. Su, Guangliang Qiang, T. Jiang, Jincun Zhao, Guizhen Wu, Jingdong Song, W. Tan   +18 more
semanticscholar   +1 more source

Competitive PCR with dual fluorescent primers enhances the specificity and reproducibility of genotyping animals generated from genome editing

Cell & Bioscience, 2023
Targeted genome editing is a powerful tool for studying gene function in almost every aspect of biological and pathological processes. The most widely used genome editing approach is to introduce engineered endonucleases or CRISPR/Cas system into cells ...
Liezhen Fu, Emily Ma, Morihiro Okada, Yuki Shibata, Yun-Bo Shi   +4 more
doaj   +1 more source

Neurotrophin signaling is a central mechanism of salivary dysfunction after irradiation that disrupts myoepithelial cells

npj Regenerative Medicine, 2023
The mechanisms that prevent regeneration of irradiated (IR) salivary glands remain elusive. Bulk RNAseq of IR versus non-IR human salivary glands showed that neurotrophin signaling is highly disrupted post-radiation.
Alejandro M. Chibly, Vaishali N. Patel, Marit H. Aure, Mary C. Pasquale, NIDCD/NIDCR Genomics and Computational Biology Core, Gemma E. Martin, Mousa Ghannam, Julianne Andrade, Noah G. Denegre, Colleen Simpson, David P. Goldstein, Fei-Fei Liu, Isabelle M. A. Lombaert, Matthew P. Hoffman   +13 more
doaj   +1 more source

The transpeptidase PBP2 governs initial localization and activity of the major cell-wall synthesis machinery in E. coli

eLife, 2020
Bacterial shape is physically determined by the peptidoglycan cell wall. The cell-wall-synthesis machinery responsible for rod shape in Escherichia coli is the processive 'Rod complex'.
Gizem Özbaykal, Eva Wollrab, Francois Simon, Antoine Vigouroux, Baptiste Cordier, Andrey Aristov, Thibault Chaze, Mariette Matondo, Sven van Teeffelen   +8 more
doaj   +1 more source