Results 71 to 80 of about 254,310 (345)

Engineering a Human‐Sized Common Bile Duct Prototype with Regenerative Potential: In Vitro Evaluation of Mechanics, Function, Degradation, and Immune Modulation

Advanced Healthcare Materials, EarlyView.
This study presents a multiphasic bile duct construct composed of biocompatible materials and cells, featuring an inner layer that supports biliary epithelium growth, a middle layer providing mechanical strength, and an outer cell‐permissive layer designed to support future in vivo integration.
Mattia Pasqua, Marianna Barbuto, Matteo Calligaris, Roberto Di Gesù, Duilio Pagano, Rosalia Busà, Salvatore Gruttadauria, Simone Dario Scilabra, Antonio D'Amore, Maria Giovanna Francipane   +9 more
wiley   +1 more source

Antitumoral Efficacy of AuNRs‐Laden ECFCs In Vitro and In Vivo: Decoding the Heat and Rays Combo Treatment in Breast Cancer and Melanoma Cells

Advanced Healthcare Materials, EarlyView.
Endothelial Colony Forming Cells (ECFCs) acted as cellular cyborgs, stealthily transporting gold nanorods (AuNRs) into tumors to enable targeted near‐infrared (NIR) hyperthermia combined with radiotherapy. This approach triggers ferroptosis in melanoma and inhibits autophagy in breast cancer, revealing a tumor‐specific response to nanomaterial‐assisted
Cecilia Anceschi, Francesca Scavone, Paolo Armanetti, Luca Menichetti, Claudia Catarinicchia, Claudia Borri, Fulvio Ratto, Filippo Micheletti, Noemi Formica, Jessica Ruzzolini, Elena Frediani, Anastasia Chillà, Francesca Margheri, Mirko Severi, Rita Traversi, Patrizia Nardini, Daniele Guasti, Mario Del Rosso, Tommaso Del Rosso, Lisa Giovanelli, Cinzia Talamonti, Monica Mangoni, Isacco Desideri, Silvia Burchielli, Fabiola Pajar, Gabriella Fibbi, Anna Laurenzana   +26 more
wiley   +1 more source

Angiogenesis is uncoupled from osteogenesis during calvarial bone regeneration

Nature Communications
Bone regeneration requires a well-orchestrated cellular and molecular response including robust vascularization and recruitment of mesenchymal and osteogenic cells.
M. Gabriele Bixel, Kishor K. Sivaraj, Melanie Timmen, Vishal Mohanakrishnan, Anusha Aravamudhan, Susanne Adams, Bong-Ihn Koh, Hyun-Woo Jeong, Kai Kruse, Richard Stange, Ralf H. Adams   +10 more
doaj   +1 more source

Tuning dCas9's ability to block transcription enables robust, noiseless knockdown of bacterial genes

Molecular Systems Biology, 2018
Over the past few years, tools that make use of the Cas9 nuclease have led to many breakthroughs, including in the control of gene expression. The catalytically dead variant of Cas9 known as dCas9 can be guided by small RNAs to block transcription of ...
Antoine Vigouroux, Enno Oldewurtel, Lun Cui, David Bikard, Sven van Teeffelen   +4 more
doaj   +1 more source

Class-A penicillin binding proteins do not contribute to cell shape but repair cell-wall defects

eLife, 2020
Cell shape and cell-envelope integrity of bacteria are determined by the peptidoglycan cell wall. In rod-shaped Escherichia coli, two conserved sets of machinery are essential for cell-wall insertion in the cylindrical part of the cell: the Rod complex ...
Antoine Vigouroux, Baptiste Cordier, Andrey Aristov, Laura Alvarez, Gizem Özbaykal, Thibault Chaze, Enno Rainer Oldewurtel, Mariette Matondo, Felipe Cava, David Bikard, Sven van Teeffelen   +10 more
doaj   +1 more source

MorphoGraphX: A platform for quantifying morphogenesis in 4D

eLife, 2015
Morphogenesis emerges from complex multiscale interactions between genetic and mechanical processes. To understand these processes, the evolution of cell shape, proliferation and gene expression must be quantified.
Pierre Barbier de Reuille, Anne-Lise Routier-Kierzkowska, D. Kierzkowski, G. Bassel, Thierry Schüpbach, G. Tauriello, N. Bajpai, Sören Strauss, Alain Weber, A. Kiss, A. Burian, Hugo Hofhuis, Aleksandra Sapala, Marcin Lipowczan, Maria B. Heimlicher, Sarah Robinson, E. Bayer, K. Basler, P. Koumoutsakos, A. Roeder, T. Aegerter-Wilmsen, N. Nakayama, M. Tsiantis, Angela Hay, D. Kwiatkowska, I. Xenarios, C. Kuhlemeier, Richard S. Smith   +27 more
semanticscholar   +1 more source

Geometrically Controlled WNT Activation Drives Intestinal Morphogenesis

Advanced Healthcare Materials, EarlyView.
Crypt‐villi‐like morphogenesis can be driven and controlled via architectural patterns in colorectal cells with the use of digital light processing printing. The resulting mini tissues exhibit budding structures and functionality, as evidenced by increased mucin expression.
Nathaniel C. Burmas, Arlyn M. Fabian, Amoli Vanavadiya, Quinton Smith   +3 more
wiley   +1 more source

High‐Resolution Patterned Delivery of Chemical Signals From 3D‐Printed Picoliter Droplet Networks

Advanced Materials, EarlyView.
3D‐printed picoliter droplet networks have been fabricated that control gene expression in bacterial populations by releasing chemical signals with precise spatial definition and high temporal resolution. This system of effector release is widely applicable, offering diverse applications in biology and medicine.
Jorin Riexinger, Thomas Caganek, Xingzao Wang, Yutong Yin, Khoa Chung, Linna Zhou, Hagan Bayley, Ravinash Krishna Kumar   +7 more
wiley   +1 more source

Analysis of Gram-negative Bacteria Peptidoglycan by Ultra-performance Liquid Chromatography

Bio-Protocol, 2020
Bacteria are surrounded by a protective peptidoglycan cell wall. Provided that this structure and the enzymes involved are the preferred target for our most successful antibiotics, determining its structural and chemical complexity is of the highest ...
Laura Alvarez, Baptiste Cordier, Sven van Teeffelen, Felipe Cava   +3 more
doaj   +1 more source

From Mechanoelectric Conversion to Tissue Regeneration: Translational Progress in Piezoelectric Materials

Advanced Materials, EarlyView.
This review highlights recent progress in piezoelectric materials for regenerative medicine, emphasizing their ability to convert mechanical stimuli into bioelectric signals that promote tissue repair. Key discussions cover the intrinsic piezoelectric properties of biological tissues, co‐stimulation cellular mechanisms for tissue regeneration, and ...
Xinyu Wang, Sílvio Terra Stefanello, Victor Shahin, Yun Qian   +3 more
wiley   +1 more source