Results 91 to 100 of about 1,109 (162)
, 2012 为了有效提高高山被孢霉(MOrTIErEllA AlPInA)产花生四烯酸的能力,采用聚乙二醇介导的原生质体转化法将重组Pd4质粒(含高山被孢霉合成花生四烯酸的延长酶基因glElO)转入高山被孢霉中,对其转化条件和同源表达进行了研究.结果表明,原生质体最佳转化条件为:以山梨醇作为稳定剂,50MMOl/l的CA2+和聚乙二醇4000作为转化介质,于25℃转化15MIn后温育,原生质体转化率最高为1.8μg-1.该转化子的摇瓶实验结果显示重组菌中花生四烯酸的产量比原始菌株的产量最高提高了近30 ...
张长杰 +3 more
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, 2017 以本课题组自主分离并鉴定的一株花生四烯酸(arachidonic acid, ARA)生产菌株,即高山被孢霉(Mortierella; alpina)LU166为研究对象,首先利用凹槽摇瓶培养,较为稳定地将菌体形态控制为刺突状小球,这一形态比絮状和大球状更有利于油脂和ARA的积累; .在此基础上优化了4种B族维生素($V{B_1}$、$V{B_{12}}$、生物素和泛酸钙)的添加量,并复合添加优化后的维生素浓度,最终获得的菌; 体生物量、油脂和ARA产量分别达到24.74,12.66和6.46 g/L ...
杨瑞琪 +3 more
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Effects of MTHFD1 overexpression on lipid synthesis in the oleaginous fungus : Mortierella alpina
, 2018 Mortierella alpina (M. alpina) is a filamentous fungus with a high polyunsaturated fatty acid content, but its sources of NADPH needed for lipid synthesis are not been studied completely.
Wang, Qizai
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Composition and biosynthesis of sterols by Mortierella alpina
, 1998 Sterols from mycelia of Mortierella alpina CBS 210.32. a zygomycetous fungus, were identified by thin layer chromatography, gas-liquid chromatography, reversed phase-high performance liquid chromatography, gas chromatography/mass spectrometry.
Nichols, Shawnn Derrek
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Nature CommunicationsLipid degradation is generally considered an antagonistic pathway to lipid synthesis, so this pathway is often removed to improve lipid production. In this study, triacylglycerol (TAG) cycling formed by lipid degradation is found to be crucial for long ...
Fei Du +8 more
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FermentationCellular residues after lipid extraction are the primary waste by-products of microbial polyunsaturated fatty acid production. To mitigate the discharge of cellular residues and reduce the cost of DHA and ARA production, this study examined the ...
Feng-Wei Yin +8 more
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, 2016 Arachidonic acid (ARA) is an important polyunsaturated fatty acid having various beneficial physiological effects on the human body. The aging of Mortierella alpina has long been known to significantly improve ARA yield, but the exact mechanism is still ...
He Huang (50693) +6 more
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Biotechnological Studies for the Production of Arachidonic Acid from Mortierella Alpina
, 2009 Mortierella alpina CBS 528.72, an oleaginous fungus and the most promising source of arachidonic acid (ARA) was exploited for production of this nutraceutically and pharmaceutically important polyunsaturated fatty acid (PUFA).
Nisha, A.
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Biotransformation of Animal Fat-By Products into ARA-Enriched Fermented Bioproducts by Solid-State Fermentation of Mortierella alpina. [PDF]
J Fungi (Basel), 2020 Slaný O +5 more
europepmc +1 more source
, 2009 Safety evaluation of arachidonic acid rich Mortierella alpina biomass was carried out in Wistar rats by acute and subchronic oral toxicity studies. A preliminary acute toxicity study revealed that the biomass was safe at acute doses and that the LD50 ...
Venkateswaran, G. +2 more
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