Condensin chromatin association is regulated by SMC head and hinge engagement and phosphorylation
Miles CA +6 more
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DNA Reshaping by MukB RIGHT-HANDED KNOTTING, LEFT-HANDED SUPERCOILING [PDF]
Journal of Biological Chemistry, 2006 MukB is a bacterial SMC (structural maintenance of chromosome) protein required for faithful chromosome segregation in Escherichia coli. We report here that purified MukB introduces right-handed knots into DNA in the presence of type-2 topoisomerase, indicating that the protein promotes intramolecular DNA condensation.
Valentin V Rybenkov
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Antagonistic Interactions of Kleisins and DNA with Bacterial Condensin MukB [PDF]
Journal of Biological Chemistry, 2006 MukBEF is a bacterial SMC (structural maintenance of chromosome) complex required for faithful chromosome segregation in Escherichia coli. The SMC subunit of the complex, MukB, promotes DNA condensation in vitro and in vivo; however, all three subunits are required for the function of MukBEF.
Valentin V Rybenkov
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Interaction of the N-terminal domain of MukB with the bacterial tubulin homologue FtsZ [PDF]
FEBS Letters, 1998 The MukB protein is involved in the process of chromosome partition in Escherichia coli and has a domain structure reminiscent of the eukaryotic motor proteins kinesin and myosin. This has led to the suggestion that MukB may function as a motor protein in vivo.
John Kendrick-Jones
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Complex formation of MukB, MukE and MukF proteins involved in chromosome partitioning in Escherichia coli [PDF]
EMBO Journal, 1999 mukF, mukE and mukB genes are essential for the process of chromosome partitioning in Escherichia coli. We have studied protein-protein interactions among MukB, MukE and MukF proteins by co-immunoprecipitation and sucrose gradient sedimentation experiments, using mukFEB null cells harboring plasmids carrying the wild-type or mutant-type mukFEB operon ...
Hironori Niki
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MukEF Is Required for Stable Association of MukB with the Chromosome [PDF]
Journal of Bacteriology, 2007 ABSTRACTMukB is a bacterial SMC(structural maintenance of chromosome) protein required for correct folding of theEscherichia colichromosome. MukB acts in complex with the two non-SMC proteins, MukE and MukF. The role of MukEF is unclear. MukEF disrupts MukB-DNA interactions in vitro.
Weifeng She, Valentin V Rybenkov
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Carboxyl terminal region of the MukB protein in Escherichia coli is essential for DNA binding activity [PDF]
FEMS Microbiology Letters, 1996 The purified MukB protein of Escherichia coli has DNA binding activity and nucleotide binding activity. We have isolated a mutation, mukB1013, causing a substitution of valine at position 1379 to leucine. This mutant MukB protein was defective for DNA binding, while the ATP binding activity remained unaffected. A truncated MukB protein that is short of
Hironori Niki, Teru Ogura
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Crystal structure of the N-terminal domain of MukB: a protein involved in chromosome partitioning [PDF]
Structure, 1999 The 170 kDa protein MukB has been implicated in ATP-dependent chromosome partitioning during cell division in Escherichia coli. MukB shares its dimeric structure and domain architecture with the ubiquitous family of SMC (structural maintenance of chromosomes) proteins that facilitate similar functions.
John Kendrick-Jones +2 more
exaly +3 more sources
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Nucleotide-Dependent Interaction of the N-Terminal Domain of MukB with Microtubules
Journal of Structural Biology, 1998The MukB protein from Escherichia coli has a domain structure that is reminiscent of the eukaryotic motor proteins kinesin and myosin: N-terminal globular domains, a region of coiled-coil, and a specialised C-terminal domain. Sequence alignment of the N-terminal domain of MukB with the kinesin motor domain indicated an approximately 22% sequence ...
John Kendrick-Jones
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The bacterial condensin MukB compacts DNA by sequestering supercoils and stabilizing topologically isolated loops [PDF]
Journal of Biological Chemistry, 2017 MukB is a structural maintenance of chromosome-like protein required for DNA condensation. The complete condensin is a large tripartite complex of MukB, the kleisin, MukF, and an accessory protein, MukE. As found previously, MukB DNA condensation is a stepwise process. We have defined these steps topologically.
Rupesh Kumar, Kenneth J Marians
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