Results 141 to 150 of about 1,534 (157)

MukB acts as a macromolecular clamp in DNA condensation

Nature Structural & Molecular Biology, 2008
Correct folding of the chromosome into its highly ordered structure requires the action of condensins. The multisubunit condensins are highly conserved from bacteria to humans, and at their core they contain the characteristic V-shaped dimer of structural maintenance of chromosome proteins.
Yuanbo, Cui, Zoya M, Petrushenko, Valentin V, Rybenkov   +2 more
openaire   +2 more sources

A Repeated Coiled-Coil Interruption in the Escherichia coli Condensin MukB

Journal of Molecular Biology, 2011
MukB, a divergent structural maintenance of chromosomes (SMC) protein, is important for chromosome segregation and condensation in Escherichia coli and other γ-proteobacteria. MukB and canonical SMC proteins share a common five-domain structure in which globular N- and C-terminal regions combine to form an ATP-binding-cassette-like ATPase domain.
Christopher S, Weitzel, Vincent M, Waldman, Travis A, Graham, Martha G, Oakley   +3 more
openaire   +2 more sources

FtsZ rings in mukB mutants with or without the Min system

Biochimie, 2001
The site of cell division in Escherichia coli is defined by formation of the Z ring between the two segregated daughter nucleoids. Positioning of the Z ring, composed of the highly conserved and tubulin-like FtsZ protein, appears to be negatively regulated by both the nucleoid and the oscillating MinCD inhibitor proteins.
X C, Yu, Q, Sun, W, Margolin
openaire   +2 more sources

Localization of replication forks in wild-type and mukB mutant cells of Escherichia coli

Molecular Genetics and Genomics, 2005
To examine the subcellular localization of the replication machinery in Escherichia coli, we have developed an immunofluorescence method that allows us to determine the subcellular location of newly synthesized DNA pulse-labeled with 5-bromo-2'-deoxyuridine (BrdU). Using this technique, we have analyzed growing cells.
Adachi, Shun, Kohiyama, Masamichi, Onogi, Toshinari, Hiraga, Sota   +3 more
openaire   +3 more sources

Comparison of MukB homodimer versus MukBEF complex molecular architectures by electron microscopy reveals a higher-order multimerization

Biochemical and Biophysical Research Communications, 2005
The complex of MukF, MukE, and MukB proteins participates in organization of sister chromosomes and partitioning into both daughter cells in Escherichia coli. We purified the MukB homodimer and the MukBEF complex and analyzed them by electron microscopy to compare both structures.
Kyoko, Matoba, Mitsuyoshi, Yamazoe, Kouta, Mayanagi, Kosuke, Morikawa, Sota, Hiraga   +4 more
exaly   +3 more sources

Crystal structure of the MukB hinge domain with coiled‐coil stretches and its functional implications

Proteins: Structure, Function, and Bioinformatics, 2009
AbstractThe structural maintenance of chromosomes (SMC) family proteins are commonly found in the multiprotein complexes involved in chromosome organization, including chromosome condensation and sister chromatid cohesion. These proteins are characterized by forming a V‐shaped homo‐ or heterodimeric structure with two long coiled‐coil arms having two ...
Ku, B, Lim, JH, Shin, HC, Shin, SY, Oh, BH   +4 more
openaire   +3 more sources

Cloning, sequencing, and characterization of multicopy suppressors of a mukB mutation in Escherichia coli

Molecular Microbiology, 1994
SummaryThe mukB gene codes for a 177kDa protein, which might be a candidate for a force‐generating enzyme in chromosome positioning in Escherichia coli. The mukB106 mutant produces normal‐sized, anucleate cells and shows a temperature‐sensitive colony formation.
K, Yamanaka, T, Mitani, T, Ogura, H, Niki, S, Hiraga   +4 more
openaire   +2 more sources

Amino acid residues for specific binding to ssDNA facilitate topological loading of bacterial condensin MukB

2023
ABSTRACTThe bacterial condensin MukB facilitates proper chromosome segregation inEscherichia coli. A portion of the MukB proteins localize at a specific chromosome region, binding to DNA in a non-sequence-specific manner. However, it is unclear how MukB localizes at a particular site without sequence specificity.
Koichiro Akiyama, Koichi Yano, Hironori Niki   +2 more
openaire   +1 more source

New killing system controlled by two genes located immediately upstream of the mukB gene in Escherichia coli

Molecular and General Genetics MGG, 1994
The nucleotide sequence was determined of the region upstream of the mukB gene of Escherichia coli. Two new genes were found, designated kicA and kicB (killing of cell); the gene order is kicB-kicA-mukB. Promoter activities were detected in the regions immediately upstream of kicB and kicA, but not in front of mukB. Gene disruption experiments revealed
J, Feng, K, Yamanaka, H, Niki, T, Ogura, S, Hiraga   +4 more
openaire   +2 more sources

Identification of Interacting Regions within the Coiled Coil of the Escherichia coli Structural Maintenance of Chromosomes Protein MukB

Journal of Molecular Biology, 2009
MukB, a divergent structural maintenance of chromosomes (SMC) protein, is important for chromosome segregation and condensation in Escherichia coli and other gamma-proteobacteria. MukB and canonical SMC proteins share a common five-domain structure in which globular N- and C-terminal regions combine to form an ABC-like ATPase domain. This ATPase domain
Yinyin, Li, Christopher S, Weitzel, Randy J, Arnold, Martha G, Oakley   +3 more
openaire   +2 more sources