Results 141 to 148 of about 1,562 (148)
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Nucleotide-Dependent Interaction of the N-Terminal Domain of MukB with Microtubules

Journal of Structural Biology, 1998
The MukB protein from Escherichia coli has a domain structure that is reminiscent of the eukaryotic motor proteins kinesin and myosin: N-terminal globular domains, a region of coiled-coil, and a specialised C-terminal domain. Sequence alignment of the N-terminal domain of MukB with the kinesin motor domain indicated an approximately 22% sequence ...
A, Lockhart, J, Kendrick-Jones
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Localization of replication forks in wild-type and mukB mutant cells of Escherichia coli

Molecular Genetics and Genomics, 2005
To examine the subcellular localization of the replication machinery in Escherichia coli, we have developed an immunofluorescence method that allows us to determine the subcellular location of newly synthesized DNA pulse-labeled with 5-bromo-2'-deoxyuridine (BrdU). Using this technique, we have analyzed growing cells.
Adachi, Shun   +3 more
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Crystal structure of the MukB hinge domain with coiled‐coil stretches and its functional implications

Proteins: Structure, Function, and Bioinformatics, 2009
AbstractThe structural maintenance of chromosomes (SMC) family proteins are commonly found in the multiprotein complexes involved in chromosome organization, including chromosome condensation and sister chromatid cohesion. These proteins are characterized by forming a V‐shaped homo‐ or heterodimeric structure with two long coiled‐coil arms having two ...
Ku, B   +4 more
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Cloning, sequencing, and characterization of multicopy suppressors of a mukB mutation in Escherichia coli

Molecular Microbiology, 1994
SummaryThe mukB gene codes for a 177kDa protein, which might be a candidate for a force‐generating enzyme in chromosome positioning in Escherichia coli. The mukB106 mutant produces normal‐sized, anucleate cells and shows a temperature‐sensitive colony formation.
K, Yamanaka   +4 more
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Amino acid residues for specific binding to ssDNA facilitate topological loading of bacterial condensin MukB

2023
ABSTRACTThe bacterial condensin MukB facilitates proper chromosome segregation inEscherichia coli. A portion of the MukB proteins localize at a specific chromosome region, binding to DNA in a non-sequence-specific manner. However, it is unclear how MukB localizes at a particular site without sequence specificity.
Koichiro Akiyama   +2 more
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Comparison of MukB homodimer versus MukBEF complex molecular architectures by electron microscopy reveals a higher-order multimerization

Biochemical and Biophysical Research Communications, 2005
The complex of MukF, MukE, and MukB proteins participates in organization of sister chromosomes and partitioning into both daughter cells in Escherichia coli. We purified the MukB homodimer and the MukBEF complex and analyzed them by electron microscopy to compare both structures.
Kyoko, Matoba   +4 more
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New killing system controlled by two genes located immediately upstream of the mukB gene in Escherichia coli

Molecular and General Genetics MGG, 1994
The nucleotide sequence was determined of the region upstream of the mukB gene of Escherichia coli. Two new genes were found, designated kicA and kicB (killing of cell); the gene order is kicB-kicA-mukB. Promoter activities were detected in the regions immediately upstream of kicB and kicA, but not in front of mukB. Gene disruption experiments revealed
J, Feng   +4 more
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Identification of Interacting Regions within the Coiled Coil of the Escherichia coli Structural Maintenance of Chromosomes Protein MukB

Journal of Molecular Biology, 2009
MukB, a divergent structural maintenance of chromosomes (SMC) protein, is important for chromosome segregation and condensation in Escherichia coli and other gamma-proteobacteria. MukB and canonical SMC proteins share a common five-domain structure in which globular N- and C-terminal regions combine to form an ABC-like ATPase domain. This ATPase domain
Yinyin, Li   +3 more
openaire   +2 more sources

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