Competitive binding of MatP and topoisomerase IV to the MukB hinge domain [PDF]
eLife, 2021 Structural Maintenance of Chromosomes (SMC) complexes have ubiquitous roles in compacting DNA linearly, thereby promoting chromosome organization-segregation.
Gemma LM Fisher +10 more
doaj +5 more sources
MukBEF-dependent chromosomal organization in widened Escherichia coli [PDF]
Frontiers in Microbiology, 2023 The bacterial chromosome is spatially organized through protein-mediated compaction, supercoiling, and cell-boundary confinement. Structural Maintenance of Chromosomes (SMC) complexes are a major class of chromosome-organizing proteins present throughout
Aleksandre Japaridze +5 more
doaj +2 more sources
DNA sliding and loop formation by E. coli SMC complex: MukBEF [PDF]
Biochemistry and Biophysics Reports, 2022 SMC (structural maintenance of chromosomes) complexes share conserved architectures and function in chromosome maintenance via an unknown mechanism. Here we have used single-molecule techniques to study MukBEF, the SMC complex in Escherichia coli.
Man Zhou
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Acyl carrier protein promotes MukBEF action in Escherichia coli chromosome organization-segregation [PDF]
Nature Communications, 2021 E. coli MukBEF is an SMC complex that plays key roles in chromosome organization-segregation. Here the authors show that the interaction between MukBEF and the Acyl Carrier Protein (AcpP) is essential for MukBEF activity in vitro (ATPase) and in vivo.
Josh P. Prince +7 more
doaj +2 more sources
Activity of MukBEF for chromosome management in E. coli and its inhibition by MatP [PDF]
eLifeStructural maintenance of chromosomes (SMC) complexes share conserved structures and serve a common role in maintaining chromosome architecture. In the bacterium Escherichia coli, the SMC complex MukBEF is necessary for rapid growth and the accurate ...
Mohammed Seba +2 more
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SMC-based immunity against extrachromosomal DNA elements. [PDF]
Biochem Soc Trans, 2023 SMC and SMC-like complexes promote chromosome folding and genome maintenance in all domains of life. Recently, they were also recognized as factors in cellular immunity against foreign DNA.
Liu HW, Roisné-Hamelin F, Gruber S.
europepmc +3 more sources
Transient growth arrest in Escherichia coli induced by chromosome condensation. [PDF]
PLoS ONE, 2013 MukB is a bacterial SMC (structural maintenance of chromosome) protein that regulates the global folding of the Escherichia coli chromosome by bringing distant DNA segments together.
Andrea L Edwards +4 more
doaj +6 more sources
Bacillus subtilis Smc condenses chromosomes in a heterologous cell system, which is down-regulated by ScpAB [PDF]
BMC Research Notes, 2020 Objective Structural maintenance of chromosomes (SMC) proteins are key players in chromosome dynamics in all types of organisms. The so-called condensin subfamily is essential for chromosome condensation in eukaryotic cells, as is the bacterial SMC ...
Tobias Knust, Peter L. Graumann
doaj +2 more sources
Self-organised segregation of bacterial chromosomal origins [PDF]
eLife, 2019 The chromosomal replication origin region (ori) of characterised bacteria is dynamically positioned throughout the cell cycle. In slowly growing Escherichia coli, ori is maintained at mid-cell from birth until its replication, after which newly ...
Andreas Hofmann +4 more
doaj +2 more sources
Loop-extrusion-mediated plasmid DNA cleavage by the bacterial SMC Wadjet complex. [PDF]
Mol CellStructural maintenance of chromosomes (SMC) complexes play pivotal roles in genome organization and maintenance across all domains of life. In prokaryotes, SMC-family Wadjet complexes structurally resemble the widespread MukBEF but serve a defensive role
Pradhan B +5 more
europepmc +3 more sources